Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Krista Witte"'
Autor:
Huddee Ho, Hong Tan, Tim Ellis, Pu Li, Juan Gamez, Michael Howes, Bettina Heidecker, Greg L. Carricato, Weilei Ma, Henrik Persson, Scott Lockard, Danfeng Yao, Jing Wei, Robert F. Zuk, Sae Choo, Janette Phi-Wilson, Michael W. Recknor, Lian-She Zhao, Ram Varma, Kevin Du, Joy Concepcion, Donald Perillat, Krista Witte, Charles Wartchow
Publikováno v:
Combinatorial Chemistry & High Throughput Screening. 12:791-800
The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f7fc122bfcc828b41ea7e231fe52cc1
https://doi.org/10.2174/138620709789104915
https://doi.org/10.2174/138620709789104915
Autor:
Steffen Nock, Krista Witte
Publikováno v:
Drug Discovery Today: Technologies. 1:35-40
The focus in the field of protein arrays has shifted from technology development to applying the technology in biological research. This review will highlight several recently published examples of biologically relevant experiments using both planar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::282256c1e61e17bbca636916b552283d
https://doi.org/10.1016/j.ddtec.2004.08.008
https://doi.org/10.1016/j.ddtec.2004.08.008
Autor:
P.-F. Indermuhle, F. G. Zaugg, T. L. Martin, Krista Witte, L. A. Ruiz-Taylor, Peter Wagner, Steffen Nock
Publikováno v:
Proceedings of the National Academy of Sciences. 98:852-857
We report on the design and characterization of a class of biomolecular interfaces based on derivatized poly( l -lysine)-grafted poly(ethylene glycol) copolymers adsorbed on negatively charged surfaces. As a model system, we synthesized biotin-deriva
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::511bc3f0ce20d6747c9929a95065cd94
https://doi.org/10.1073/pnas.98.3.852
https://doi.org/10.1073/pnas.98.3.852
Publikováno v:
Biopolymers. 60:220-228
The problem of identifying downstream targets of kinase phosphorylation remains a challenge despite technological advances in genomics and proteomics. A recent approach involves the generation of kinase mutants that can uniquely use "orthogonal" ATP
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3e724b4af83ae8c46e2ceb0b6333fa38
https://doi.org/10.1002/1097-0282(2001)60:3<220::aid-bip10035>3.0.co
https://doi.org/10.1002/1097-0282(2001)60:3<220::aid-bip10035>3.0.co
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 6:297-304
The stability of subtilisin BPN′ in organic solvents or cosolvent/water mixtures was studied as a function of the type and concentration of counterion at the time of freeze-drying, water concentration, and stirring speed/method. It was found that t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9c555a47dc56c556e2e59be983f35021
https://doi.org/10.1016/s1381-1177(98)00085-x
https://doi.org/10.1016/s1381-1177(98)00085-x
Publikováno v:
Journal of the American Chemical Society. 120:13042-13045
A recent computational analysis of retro-proteins (backward reading of native proteins) suggests that the retro-protein has a tendency to adopt a structure similar to that of the natural one, as de...
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::abad7c7ef8190a3b81f9e8350450c367
https://doi.org/10.1021/ja982203h
https://doi.org/10.1021/ja982203h
Publikováno v:
Bioorganic & Medicinal Chemistry. 6:1283-1292
Methods have been developed for the enzymatic synthesis of complex carbohydrates and glycoproteins containing in the sialic acid moiety the heavy metal mercury or the transition-state analog phosphonate of the influenza C 9-O-acetyl-neuraminic acid e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::065a58999c7072ea5921a5fa667a3c26
https://doi.org/10.1016/s0968-0896(98)00121-7
https://doi.org/10.1016/s0968-0896(98)00121-7
Publikováno v:
Journal of the American Chemical Society. 120:1979-1989
This paper describes the solution- and solid-phase synthesis of glycopeptides containing a GlcNAc moiety at different positions and investigates them as substrates for subtilisin-catalyzed glycopeptide condensation, with an aim to develop enzymatic s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6432ebb4acac1081c6e5f149518194bd
https://doi.org/10.1021/ja9736727
https://doi.org/10.1021/ja9736727
Publikováno v:
Journal of the American Chemical Society. 119:3942-3947
The ability of the serine protease subtilisin BPN‘ to catalyze peptide bond formation between fragments containing noncoded amino acids, peptide mimetics, and peptide conjugates in a kinetic approach was explored. It was found that the enzyme accep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::584d825424fef8a097005aae5389ce50
https://doi.org/10.1021/ja964399w
https://doi.org/10.1021/ja964399w
Publikováno v:
Journal of the American Chemical Society. 119:2114-2118
In order to study the effects carbohydrates have on glycoprotein structure and function, it is imperative to be able to synthesize the appropriate natural and non-natural glycoprotein variants in a single form. Because the available in vivo technique
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::049212499df320a261e9c0146cc1cb6d
https://doi.org/10.1021/ja961846z
https://doi.org/10.1021/ja961846z