Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Krista L. Morley"'
Autor:
Jordan P. Volpato, Krista L. Morley, Maximilian C. C. J. C. Ebert, Joelle N. Pelletier, Andreea R. Schmitzer
Publikováno v:
Protein Science. 24:495-507
Type II R67 dihydrofolate reductase (DHFR) is a bacterial plasmid-encoded enzyme that is intrinsically resistant to the widely-administered antibiotic trimethoprim. R67 DHFR is genetically and structurally unrelated to E. coli chromosomal DHFR and ha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::67282dc31458caf4b535a3c8c06ea4c1
https://doi.org/10.1002/pro.2602
https://doi.org/10.1002/pro.2602
Autor:
Peter Bernhardt, Joseph D. Schrag, Vincent M. Purpero, Krista L. Morley, Jeremy D. Cheeseman, Yun Jiang, De Lu Tyler Yin, Romas J. Kazlauskas
Publikováno v:
Biochemistry. 49:1931-1942
Many serine hydrolases catalyze perhydrolysis, the reversible formation of peracids from carboxylic acids and hydrogen peroxide. Recently, we showed that a single amino acid substitution in the alcohol binding pocket, L29P, in Pseudomonas fluorescens
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d68af64d916cc70c2717ff96fcb78fdb
https://doi.org/10.1021/bi9021268
https://doi.org/10.1021/bi9021268
Autor:
François Shareck, Grégory Chauve, Romas J. Kazlauskas, Claude Dupont, Robert H. Marchessault, Krista L. Morley
Publikováno v:
Carbohydrate Polymers. 63:310-315
Acetyl xylan esterase catalyzes the hydrolysis of N-acetyl groups in chitinous materials of variable degrees of polymerization and acetylation. The influence of substrate accessibility is most notable with substrate of high degree of acetylation (DA)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1250d8b0df9d0077e112874cc31bd15e
https://doi.org/10.1016/j.carbpol.2005.07.034
https://doi.org/10.1016/j.carbpol.2005.07.034
Publikováno v:
Tetrahedron: Asymmetry. 15:3005-3009
Use of solid-supported reagents simplified routine acylation of primary and secondary alcohols because it eliminated traditional purification. Using a parallel synthesizer, eight primary or secondary alcohols reacted with acid chloride in the presenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c443f5c0a43d3eee3a339b559198cab5
https://doi.org/10.1016/j.tetasy.2004.07.048
https://doi.org/10.1016/j.tetasy.2004.07.048
Autor:
Maximilian C C J C, Ebert, Krista L, Morley, Jordan P, Volpato, Andreea R, Schmitzer, Joelle N, Pelletier
Publikováno v:
Protein science : a publication of the Protein Society. 24(4)
Type II R67 dihydrofolate reductase (DHFR) is a bacterial plasmid-encoded enzyme that is intrinsically resistant to the widely-administered antibiotic trimethoprim. R67 DHFR is genetically and structurally unrelated to E. coli chromosomal DHFR and ha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::51681e67dd816e312cfd628d10e133dd
https://pubmed.ncbi.nlm.nih.gov/25401264
https://pubmed.ncbi.nlm.nih.gov/25401264
Publikováno v:
Chembiochem : a European journal of chemical biology. 12(5)
Acyl transfer is a key reaction in biosynthesis, including synthesis of antibiotics and polyesters. Although researchers have long recognized the similar protein fold and catalytic machinery in acyltransferases and hydrolases, the molecular basis for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3775ee473cc7dce5c30f8b7caa1b7db9
https://pubmed.ncbi.nlm.nih.gov/21351219
https://pubmed.ncbi.nlm.nih.gov/21351219
Autor:
Audrey Nisole, François Shareck, Claude Dupont, Joelle N. Pelletier, Romas J. Kazlauskas, François Xavier Lussier, Krista L. Morley
Publikováno v:
Protein expression and purification. 46(2)
Acetyl xylan esterase A (AxeA) from Streptomyces lividans belongs to a large family of industrially relevant polysaccharide esterases. AxeA and its truncated form containing only the catalytically competent domain, AxeA(tr), catalyze both the deacety
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63cabd5dafc6c0a6cd56303a3c7414b7
https://pubmed.ncbi.nlm.nih.gov/16256365
https://pubmed.ncbi.nlm.nih.gov/16256365
Autor:
Krista L. Morley, Romas J. Kazlauskas
Publikováno v:
Trends in biotechnology. 23(5)
Study of mutations that improve enzyme properties reveals that in many, but not all, cases closer mutations are more effective than distant ones. For enantioselectivity, substrate selectivity and new catalytic activity (catalytic promiscuity) closer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d48e8254e7b9a69aa139d673934e673
https://pubmed.ncbi.nlm.nih.gov/15866000
https://pubmed.ncbi.nlm.nih.gov/15866000
Publikováno v:
ChemBioChem. 12:654-654
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4f6161f9105f77e3631617f04e184cc5
https://doi.org/10.1002/cbic.201190016
https://doi.org/10.1002/cbic.201190016