Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Krishna S Ghanta"'
Autor:
Krishna S Ghanta, Zexiang Chen, Aamir Mir, Gregoriy A Dokshin, Pranathi M Krishnamurthy, Yeonsoo Yoon, Judith Gallant, Ping Xu, Xiao-Ou Zhang, Ahmet Rasit Ozturk, Masahiro Shin, Feston Idrizi, Pengpeng Liu, Hassan Gneid, Alireza Edraki, Nathan D Lawson, Jaime A Rivera-Pérez, Erik J Sontheimer, Jonathan K Watts, Craig C Mello
Publikováno v:
eLife, Vol 10 (2021)
Nuclease-directed genome editing is a powerful tool for investigating physiology and has great promise as a therapeutic approach to correct mutations that cause disease. In its most precise form, genome editing can use cellular homology-directed repa
Externí odkaz:
https://doaj.org/article/a4d2a8d974e94e189ee441394236bb61
Autor:
Samuel F. Kingsley, Yonghak Seo, Calista Allen, Krishna S. Ghanta, Steven Finkel, Heidi A. Tissenbaum
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
Abstract Intestinal microbiota play an essential role in the health of a host organism. Here, we define how commensal Escherichia coli (E. coli) alters its host after long term exposure to glucose using a Caenorhabditis elegans-E. coli system where o
Externí odkaz:
https://doaj.org/article/f63aae9db4f64f86b521e0dee1a092a0
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100748- (2021)
Summary: In Caenorhabditis elegans, targeted genome editing techniques are now routinely used to generate germline edits. The remarkable ease of C. elegans germline editing is attributed to the syncytial nature of the pachytene ovary which is easily
Externí odkaz:
https://doaj.org/article/63f301d8666f4e448b10e5414363036d
Autor:
Krishna S. Ghanta, Craig C. Mello
Publikováno v:
Genetics
Melting and fast cooling double stranded DNA donor molecules prior to injection dramatically increases the frequency of homology-directed repair for edits such as insertions of fluorescent protein markers in Caenorhabditis elegans. Strategies describ
Autor:
Craig C. Mello, Yeonsoo Yoon, Hassan Gneid, Jonathan K. Watts, Jaime A. Rivera-Pérez, Ping Xu, Pengpeng Liu, Xiao-Ou Zhang, Judith Gallant, Krishna S. Ghanta, Masahiro Shin, Nathan D. Lawson, Pranathi Meda Krishnamurthy, Ahmet R. Ozturk, Aamir Mir, Erik J. Sontheimer, Zexiang Chen, Alireza Edraki, Gregoriy A Dokshin, Feston Idrizi
Publikováno v:
eLife
eLife, Vol 10 (2021)
eLife, Vol 10 (2021)
Nuclease-directed genome editing is a powerful tool for investigating physiology and has great promise as a therapeutic approach to correct mutations that cause disease. In its most precise form, genome editing can use cellular homology-directed repa
Autor:
Hassan Gneid, Zexiang Chen, Xiao-Ou Zhang, Krishna S. Ghanta, Judith Gallant, Pranathi Meda Krishnamurthy, Gregoriy A Dokshin, Ping Xu, Masahiro Shin, Jonathan K. Watts, Aamir Mir, Erik J. Sontheimer, Pengpeng Liu, Ahmet R. Ozturk, Craig C. Mello, Feston Idrizi, Alireza Edraki, Yeonsoo Yoon, Nathan D. Lawson, Jaime A. Rivera-Pérez
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d121f3c8a2d65cde404482314c798819
https://doi.org/10.7554/elife.72216.sa2
https://doi.org/10.7554/elife.72216.sa2
Autor:
Heidi A. Tissenbaum, Yong-Hak Seo, Steven E. Finkel, Samuel F. Kingsley, Krishna S. Ghanta, Calista Allen
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
Scientific Reports
Scientific Reports
Intestinal microbiota play an essential role in the health of a host organism. Here, we define how commensal Escherichia coli (E. coli) alters its host after long term exposure to glucose using a Caenorhabditis elegans-E. coli system where only the b
Autor:
Yong-Hak Seo, Heidi A. Tissenbaum, Samuel F. Kingsley, Calista Allen, Steven E. Finkel, Krishna S. Ghanta
Intestinal microbiota play an essential role in the health of a host organism. Here, we define how commensal Escherichia coli (E. coli) alters its host after long term exposure to glucose using a C. elegans-E. coli system. Our data reveal that bacter
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::40694e7d93403e214b8057abf3b3c9ef
https://doi.org/10.1101/2020.09.13.295451
https://doi.org/10.1101/2020.09.13.295451
Autor:
Krishna S. Ghanta, Craig C. Mello
CRISPR genome editing has revolutionized genetics in many organisms. In the nematode Caenorhabditis elegans one injection into each of the two gonad arms of an adult hermaphrodite exposes hundreds of meiotic germ cells to editing mixtures, permitting
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::45e862e5fcf0ea7752531b445e5cd6bd
https://doi.org/10.1101/2020.08.03.235036
https://doi.org/10.1101/2020.08.03.235036
Publikováno v:
Genetics. 210:781-787
A robust genome editing pipeline is critical to the vitality of a modern genetic laboratory. Previous studies have shown that Cas9 ribonucleoprotein (RNP)-based editing can be highly effective in Caenorhabditis elegans, particularly... CRISPR-based g