Comparison of gel-aided sample preparation (GASP) and two in-solution digestion workflows for proteomic analysis using Saccharomyces cerevisiae lysate

Autor: Jailani, NM, Sani, AA, Fischer, R, Huang, H, Aizat, WM, Nathan, S, Kramer, HB, Kessler, BM, Mackeen, MM

Using Saccharomyces cerevisiae lysate, two in-solution trypsin digestions (chloroform-methanol-water precipitation and RapiGest) were compared to the recently reported gel-aided sample preparation (GASP) workflow. Our proteomic results showed that GA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::c2b99d30fdfdc102fbd0bac9d9e406c3
https://doi.org/10.21161/mjm.1461817

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Disrupted propionate metabolism evokes transcriptional changes in the heart by increasing histone acetylation and propionylation.

Autor: Park KC; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK., Crump NT; MRC Molecular Haematology Unit, Radcliffe Department of Medicine, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK., Louwman N; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK., Krywawych S; Department of Chemical Pathology, Great Ormond Street Hospital NHS Foundation Trust, London, UK., Cheong YJ; Epigenetics & Signalling Programmes, Babraham Institute, Cambridge, UK., Vendrell I; Nuffield Department of Medicine, Target Discovery Institute, Oxford, UK.; Nuffield Department of Medicine, Chinese Academy for Medical Sciences Oxford Institute, University of Oxford, Oxford, UK., Gill EK; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK., Gunadasa-Rohling M; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK., Ford KL; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK., Hauton D; Department of Chemistry, University of Oxford, Oxford, UK., Fournier M; Department of Biochemistry, University of Oxford, Oxford, UK., Pires E; Department of Chemistry, University of Oxford, Oxford, UK., Watson L; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK., Roseman G; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK., Holder J; Department of Biochemistry, University of Oxford, Oxford, UK., Koschinski A; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK., Carnicer R; Division of Cardiovascular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, UK., Curtis MK; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK., Zaccolo M; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK., Hulikova A; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK., Fischer R; Nuffield Department of Medicine, Target Discovery Institute, Oxford, UK.; Nuffield Department of Medicine, Chinese Academy for Medical Sciences Oxford Institute, University of Oxford, Oxford, UK., Kramer HB; MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK., McCullagh JSO; Department of Chemistry, University of Oxford, Oxford, UK., Trefely S; Epigenetics & Signalling Programmes, Babraham Institute, Cambridge, UK., Milne TA; MRC Molecular Haematology Unit, Radcliffe Department of Medicine, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK., Swietach P; Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, UK.

Publikováno v: Nature cardiovascular research [Nat Cardiovasc Res] 2023 Dec; Vol. 2, pp. 1221-1245. Date of Electronic Publication: 2023 Nov 23.

Factor inhibiting HIF can catalyze two asparaginyl hydroxylations in VNVN motifs of ankyrin fold proteins.

Autor: Leissing TM; Chemistry Research Laboratory, Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research, University of Oxford, Oxford, United Kingdom; Ludwig Institute for Cancer Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom., Hardy AP; Chemistry Research Laboratory, Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research, University of Oxford, Oxford, United Kingdom., Chan H; Ludwig Institute for Cancer Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom., Wang Y; Ludwig Institute for Cancer Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom., Tumber A; Chemistry Research Laboratory, Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research, University of Oxford, Oxford, United Kingdom., Chowdhury R; Chemistry Research Laboratory, Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research, University of Oxford, Oxford, United Kingdom., Feng T; Ludwig Institute for Cancer Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom; NDM Research Building, University of Oxford, Oxford, United Kingdom., Coleman ML; Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, United Kingdom., Cockman ME; The Francis Crick Institute, Ratcliffe Laboratory, London, United Kingdom., Kramer HB; MRC London Institute of Medical Sciences, London, United Kingdom; Institute of Clinical Sciences, Imperial College London, Hammersmith Hospital Campus, London, United Kingdom., Berridge G; NDM Research Building, University of Oxford, Oxford, United Kingdom., Fischer R; NDM Research Building, University of Oxford, Oxford, United Kingdom., Kessler BM; NDM Research Building, University of Oxford, Oxford, United Kingdom., Ratcliffe PJ; Ludwig Institute for Cancer Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom; The Francis Crick Institute, Ratcliffe Laboratory, London, United Kingdom. Electronic address: peter.ratcliffe@ndm.ox.ac.uk., Lu X; Ludwig Institute for Cancer Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom. Electronic address: xin.lu@ludwig.ox.ac.uk., Schofield CJ; Chemistry Research Laboratory, Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research, University of Oxford, Oxford, United Kingdom. Electronic address: christopher.schofield@chem.ox.ac.uk.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2022 Jun; Vol. 298 (6), pp. 102020. Date of Electronic Publication: 2022 May 07.