Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing.

Autor: Ignatov KB; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow, Russia.; Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia., Blagodatskikh KA; Evrogen JSC, Moscow, Russia.; Pirogov Russian National Research Medical University, Moscow, Russia., Shcherbo DS; Pirogov Russian National Research Medical University, Moscow, Russia., Kramarova TV; The Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden., Monakhova YA; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow, Russia.; Syntol JSC, Moscow, Russia., Kramarov VM; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow, Russia.; Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.

Publikováno v: PloS one [PLoS One] 2019 Apr 01; Vol. 14 (4), pp. e0210374. Date of Electronic Publication: 2019 Apr 01 (Print Publication: 2019).

The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen.

Autor: Shchit IY; 1State Research Center of Applied Microbiology and Biotechnology, Federal Service for the Protection of Customer Rights, Obolensk, 142279 Russia., Ignatov KB; 2Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 117971 Russia.; 3All-Russian Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow, 127422 Russia., Kudryavtseva TY; 1State Research Center of Applied Microbiology and Biotechnology, Federal Service for the Protection of Customer Rights, Obolensk, 142279 Russia., Shishkova NA; 1State Research Center of Applied Microbiology and Biotechnology, Federal Service for the Protection of Customer Rights, Obolensk, 142279 Russia., Mironova RI; 1State Research Center of Applied Microbiology and Biotechnology, Federal Service for the Protection of Customer Rights, Obolensk, 142279 Russia., Marinin LI; 1State Research Center of Applied Microbiology and Biotechnology, Federal Service for the Protection of Customer Rights, Obolensk, 142279 Russia., Mokrievich AN; 1State Research Center of Applied Microbiology and Biotechnology, Federal Service for the Protection of Customer Rights, Obolensk, 142279 Russia., Kramarov VM; 2Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 117971 Russia.; 3All-Russian Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow, 127422 Russia., Biketov SF; 1State Research Center of Applied Microbiology and Biotechnology, Federal Service for the Protection of Customer Rights, Obolensk, 142279 Russia., Dyatlov IA; 1State Research Center of Applied Microbiology and Biotechnology, Federal Service for the Protection of Customer Rights, Obolensk, 142279 Russia.

Publikováno v: Molecular genetics, microbiology and virology : Molekulyarnaya genetika, mikrobiologiya i virusologiya [Mol Gen Microbiol Virol] 2017; Vol. 32 (2), pp. 100-108. Date of Electronic Publication: 2017 Sep 20.

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A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification.

Autor: Ignatov KB; Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia; All-Russia Institute of Agricultural Biotechnology, Moscow, Russia., Barsova EV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; Evrogen JSC, Moscow, Russia., Fradkov AF; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; Evrogen JSC, Moscow, Russia., Blagodatskikh KA; All-Russia Institute of Agricultural Biotechnology, Moscow, Russia; Syntol JSC, Moscow, Russia., Kramarova TV; The Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden., Kramarov VM; Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia; All-Russia Institute of Agricultural Biotechnology, Moscow, Russia.

Publikováno v: BioTechniques [Biotechniques] 2014 Aug 01; Vol. 57 (2), pp. 81-7. Date of Electronic Publication: 2014 Aug 01 (Print Publication: 2014).

DNA ligases from thermophilic bacteria enhance PCR amplification of long DNA sequences.

Autor: Ignatov KB; Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia. ignatovkb@bk.ru, Kramarov VM

Publikováno v: Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2009 May; Vol. 74 (5), pp. 557-61.