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Absolute quantitation of proteins by Acid hydrolysis combined with amino Acid detection by mass spectrometry

Autor: Mirgorodskaya, Olga A, Körner, Roman, Kozmin, Yuri P, Roepstorff, Peter

Publikováno v: Mirgorodskaya, O A, Körner, R, Kozmin, Y P & Roepstorff, P 2012, ' Absolute quantitation of proteins by Acid hydrolysis combined with amino Acid detection by mass spectrometry ', Methods in Molecular Biology, vol. 828, pp. 115-20 . https://doi.org/10.1007/978-1-61779-445-2_11

Amino acid analysis is among the most accurate methods for absolute quantification of proteins and peptides. Here, we combine acid hydrolysis with the addition of isotopically labeled standard amino acids and analysis by mass spectrometry for accurat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______3062::b55e629ee463c5f63b56cbb8aa0af4b2
https://portal.findresearcher.sdu.dk/da/publications/a78c0896-9326-493f-8b3c-928285591512

Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry.

Autor: Mirgorodskaya, Olga A., Körner, Roman, Kozmin, Yuri P., Roepstorff, Peter

Publikováno v: Amino Acid Analysis; 2012, p115-120, 6p

The N-domain of angiotensin-converting enzyme specifically hydrolyzes the Arg-5-His-6 bond of Alzheimer's Aβ-(1-16) peptide and its isoAsp-7 analogue with different efficiency as evidenced by quantitative matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Autor: Toropygin, Ilya Yu., Kugaevskaya, Elena V., Mirgorodskaya, Olga A., Elisseeva, Yulia E., Kozmin, Yuri P., Popov, Igor A., Nikolaev, Eugene N., Makarov, Alexander A., Kozin, Sergey A.

Publikováno v: Rapid Communications in Mass Spectrometry: RCM; Jan2008, Vol. 22 Issue 2, p231-239, 9p