A high performance Trichoderma reesei strain that reveals the importance of xylanase III in cellulosic biomass conversion

Autor: Jun-ichi Sumitani, Hikaru Nakazawa, Yasushi Morikawa, Takashi Kawaguchi, Tetsushi Kawai, Noriko Ida, Wataru Ogasawara, Shuji Tani, Yosuke Shida, Hirofumi Okada, Yoshinori Kobayashi, Kouki Shioya

Publikováno v: Enzyme and Microbial Technology. 82:89-95

The ability of the Trichoderma reesei X3AB1strain enzyme preparations to convert cellulosic biomass into fermentable sugars is enhanced by the replacement of xyn3 by Aspergillus aculeatus β-glucosidase 1 gene (aabg1), as shown in our previous study.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::571a0b91cf392df712be3a84e7126c26
https://doi.org/10.1016/j.enzmictec.2015.08.019

Evaluation of essential and variable residues of nukacin ISK-1 by NNK scanning

Autor: Jun-ichi Nagao, Takeshi Zendo, Jiro Nakayama, Mohammad Riazul Islam, Mami Nishie, Kouki Shioya, Kenji Sonomoto, Hiroyuki Jikuya

Publikováno v: Molecular Microbiology. 72:1438-1447

Summary Nukacin ISK-1, a type-A(II) lantibiotic, comprises 27 amino acids with a distinct linear N-terminal and a globular C-terminal region. To identify the positional importance or redundancy of individual residues responsible for nukacin ISK-1 ant

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6aefe5312f2b4bd04a498d93d7baae83
https://doi.org/10.1111/j.1365-2958.2009.06733.x

Lanthionine introduction into nukacin ISK-1 prepeptide by co-expression with modification enzyme NukM in Escherichia coli

Autor: Yoshitaka Harada, Takeshi Zendo, Kenji Sonomoto, Kouki Shioya, Jiro Nakayama, Yuji Aso, Jun Ichi Nagao

Publikováno v: Biochemical and Biophysical Research Communications. 336:507-513

We demonstrated lanthionine introduction into hexa-histidine-tagged (His-tagged) nukacin ISK-1 prepeptide NukA by modification enzyme NukM in Escherichia coli. Co-expression of nukA and nukM, purification of the resulting His-tagged prepeptide by aff

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ba9a8c6de3f1b5fe3db272ad8a9b081
https://doi.org/10.1016/j.bbrc.2005.08.125

Genome-Wide Identification of Small RNAs in the Opportunistic Pathogen Enterococcus faecalis V583

Autor: Carsten Kuenne, Axel Hartke, Kouki Shioya, Aurélie Budin-Verneuil, Charlotte Michaux, Jean-Christophe Giard, Thomas Hartsch, Torsten Hain, Nicolas Verneuil

Publikováno v: PLoS ONE
PLoS ONE, Public Library of Science, 2011, 6 (9), pp.e23948. ⟨10.1371/journal.pone.0023948⟩
PLoS ONE, Vol 6, Iss 9, p e23948 (2011)
Plos One 9 (6), . (2011)

International audience; Small RNA molecules (sRNAs) are key mediators of virulence and stress inducible gene expressions in some pathogens. In this work we identify sRNAs in the Gram positive opportunistic pathogen Enterococcus faecalis. We character

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a86cde20a299d9632287f465b39ef2c2
https://hal-normandie-univ.archives-ouvertes.fr/hal-02180935

Engineering Unusual Amino Acids into Peptides Using Lantibiotic Synthetase

Autor: Ken Ichi Okuda, Jun-ichi Nagao, Takeshi Zendo, Yoshitaka Harada, Kenji Sonomoto, Jiro Nakayama, Kouki Shioya

Publikováno v: Methods in Molecular Biology ISBN: 9781617379666

Alteration of protein structure and function by introducing unusual amino acids has great potential to develop new biological tool and to produce novel therapeutic agents. Lantibiotics produced by Gram-positive bacteria are ribosomally synthesized an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::71355b27d0cf349967739336e1a40af9
https://doi.org/10.1007/978-1-61737-967-3_13