Výsledky vyhledávání - "Kouichi Takeshi"

Prevalence of foodborne pathogens in open markets and supermarkets in Thailand

Autor: Wanpen Chaicumpa, Keiko Kawamoto, Manas Chongsa-nguan, Atsuka Minami, Sou-ichi Makino, Shuko Monden, Seksun Samosornsuk, Kouichi Takeshi

Publikováno v: Food Control. 21:221-226

This study was conducted in Thailand (Bangkok and Pathum Thani provinces), from June 2006 to July 2007, in order to assess the prevalence of Listeria monocytogenes, Escherichia coli O157, Salmonella, Shigella and Vibrio parahaemolyticus in foods. Ret

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::19dba10e12b3038d6ab9b7f34c13eeeb
https://doi.org/10.1016/j.foodcont.2009.05.011

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Characterization of Toxin Complex Produced by a Unique Strain of Clostridium botulinum Serotype D 4947

Autor: Keiji Oguma, Kimiko Hasegawa, Akihito Yamano, Tomonori Suzuki, Toshihiro Watanabe, Yukako Fujinaga, Tohru Ohyama, Kouichi Takeshi, Arihide Kamaguchi, Shingo Mutoh, Hirokazu Kouguchi, Yoshimasa Sagane, Hiroaki Sato

Publikováno v: The Protein Journal. 23:371-378

A unique strain of Clostridium botulinum, serotype D 4947 (D-4947), produces a considerable amount of a 650 kDa toxin complex (L-TC) and a small amount of a 280 kDa M-TC, a 540 kDa TC, and a 610 kDa TC. The complexes are composed of only un-nicked co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb9c7e84a13af3b0514cd97d07da7167
https://doi.org/10.1023/b:jopc.0000039551.42676.4c

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A Simple and Sensitive Polymerase Chain Reaction Method for the Detection of Food-Related Bacteria

Autor: Kouichi Takeshi, Yukihiro Okumura, Takeshi Shimizu, Daisuke Yasokawa, Koji Nagashima, Ryoji Nakagawa, Makoto Kawakami

Publikováno v: Food Science and Technology Research. 6:115-118

We devised a simple and efficient method of preparing chromosomal DNA from Gram-positive bacteria for use as a template in PCR, which consists of achromopeptidase treatment followed by Chelex 100 treatment. Using template DNA prepared by this method,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b7d92df42e886902ce9e57889cfbb871
https://doi.org/10.3136/fstr.6.115

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Characterization of haemagglutinin activity of Clostridium botulinum type C and D 16S toxins, and one subcomponent of haemagglutinin (HA1)

Autor: Kenji Yokota, Kouichi Takeshi, Yukako Fujinaga, Keiji Oguma, Koichi Honke, Toshihiro Watanabe, Kaoru Inoue, Tohru Ohyama, Tetsuya Ikeda, Katsuhiro Inoue

Publikováno v: Microbiology. 145:2533-2542

The 16S toxin and one subcomponent of haemagglutinin (HA), designated HA1, were purified from a type D culture of Clostridium botulinum by a newly established procedure, and their HA activities as well as that of purified type C 16S toxin were charac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73abd2584c5e5c05dd5dfdcccbe7cb66
https://doi.org/10.1099/00221287-145-9-2533

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Detection and Genetical Characterization of Shiga Toxin-ProducingEscherichia colifrom Wild Deer

Autor: Kouichi Takeshi, Teizo Tsukamoto, Hisao Kurazono, Toshikazu Shirahata, Sou-ichi Makino, Hiroshi Asakura, Tetsuya Ikeda

Publikováno v: Microbiology and Immunology. 42:815-822

Shiga toxin (Stx)-producing Escherichia coli (STEC) strains isolated from wild deer in Japan were examined. A total of 43 fecal samples were collected 4 times from 4 different sites around Obihiro City, Hokkaido, Japan, in June and July 1997. Seven S

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb20e585fce4717dabeaee467e167d29
https://doi.org/10.1111/j.1348-0421.1998.tb02356.x

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Simple Method for Detection ofClostridium botulinumType A to F Neurotoxin Genes by Ploymerase Chain Reaction

Autor: Kaoru Inoue, Keiji Oguma, Hiroyuki Sunagawa, Tetsuya Ueno, Hiroshi Nakajima, Yukako Fujinaga, Kouichi Takeshi, Tohru Ohyama

Publikováno v: Microbiology and Immunology. 40:5-11

A polymerase chain reaction (PCR)-based method was established to detect each type of neurotoxin genes of Clostridium botulinum types A to F by employing the oligonucleotide primer sets corresponding to special regions of the light chains of the neur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e01530621bf109324b028ec29febcbb2
https://doi.org/10.1111/j.1348-0421.1996.tb03310.x

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Type A and B Neurotoxin Genes in a Clostridium botulinum Type AB Strain

Autor: R. Fujita, Keiji Oguma, Kaoru Inoue, K Moriishi, Yukako Fujinaga, Kouichi Takeshi, Tohru Ohyama

Publikováno v: Biochemical and Biophysical Research Communications. 213:737-745

The genetic structure of neurotoxin genes in a Clostridium botulinum strain producing both type A and B neurotoxins (type AB) was investigated. Analyses by polymerase chain reaction (PCR) using type-specific primers corresponding to the coding region

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8c8ffe7bccb210de4abd922bf3eae3d
https://doi.org/10.1006/bbrc.1995.2192

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Autor: Emiko Isogai, Hiroshi Isogai, Nobuhiro Fujii, Kouichi Kimura, Kouichi Takeshi, Noriko Yokosawa, Keiji Oguma, Teruo Yashiki, Touru Ohyama

Publikováno v: Microbiology and Immunology. 37:395-398

The complete nucleotide and deduced amino acid sequence of the nontoxic component of botulinum type E progenitor toxin is determined in recombinant plasmid pU9BUH containing about 6.0 kb HindIII fragment obtained from chromosomal DNA of Clostridium b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f92c73c2262ef7dd9e602e4c3400d6e3
https://doi.org/10.1111/j.1348-0421.1993.tb03227.x

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Characterization of NeurotoxigenicClostridium butyricumStrain by DNA Hybridization Test and byin vivoandin vitroGermination Tests of Spores

Autor: Keiji Oguma, Shinichi Nakamura, Kouichi Takeshi, Nobuhiro Fujii, Kiyotaka Yamakawa, Kouichi Kimura, Mitsuru Kumagai

Publikováno v: Microbiology and Immunology. 35:1095-1103

The germination of spores of a neurotoxigenic Clostridium butyricum strain (BL 6340), which was isolated from infant botulism in Italy, and that of a nontoxigenic C. butyricum type strain (NCIB 7423) were studied. The spores of BL 6340 strain were ki

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72470c3b37d42fbd82e8183421512a24
https://doi.org/10.1111/j.1348-0421.1991.tb01631.x

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