Zobrazeno 1 - 3
of 3
pro vyhledávání: '"Kota Sugio"'
Autor:
Deshani C. Ranawakage, Keita Okada, Kota Sugio, Yuya Kawaguchi, Yuki Kuninobu-Bonkohara, Takuya Takada, Yusuke Kamachi
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 8 (2021)
Despite the unprecedented gene editing capability of CRISPR-Cas9-mediated targeted knock-in, the efficiency and precision of this technology still require further optimization, particularly for multicellular model organisms, such as the zebrafish (Da
Externí odkaz:
https://doaj.org/article/0c8dc2dc68eb4a5ebc6159214f78efde
Autor:
Keita Okada, Kanae Aoki, Teruyuki Tabei, Kota Sugio, Katsunori Imai, Yuki Bonkohara, Yusuke Kamachi
Publikováno v:
Nucleic Acids Research. 50:2854-2871
Specific sequence features of the protospacer and protospacer-adjacent motif (PAM) are critical for efficient cleavage by CRISPR-Cas9, but current knowledge is largely derived from single-guide RNA (sgRNA) systems assessed in cultured cells. In this
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2a5cf89db35c48aebf55408cfacaa8d
https://doi.org/10.1093/nar/gkac100
https://doi.org/10.1093/nar/gkac100
Autor:
Deshani C. Ranawakage, Takuya Takada, Yuki Kuninobu-Bonkohara, Yusuke Kamachi, Keita Okada, Yuya Kawaguchi, Kota Sugio
Publikováno v:
Frontiers in Cell and Developmental Biology
Frontiers in Cell and Developmental Biology, Vol 8 (2021)
Frontiers in Cell and Developmental Biology, Vol 8 (2021)
Despite the unprecedented gene editing capability of CRISPR-Cas9-mediated targeted knock-in, the efficiency and precision of this technology still require further optimization, particularly for multicellular model organisms, such as the zebrafish (Da
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c79710d470cd5a01fd666a2e43e4c8e9
https://pubmed.ncbi.nlm.nih.gov/33681181
https://pubmed.ncbi.nlm.nih.gov/33681181