Zobrazeno 1 - 10
of 53
pro vyhledávání: '"Kota Mizumoto"'
Autor:
Mizuki Kurashina, Kota Mizumoto
Publikováno v:
STAR Protocols, Vol 4, Iss 1, Pp 102028- (2023)
Summary: The auxin-inducible degron (AID) provides reversible, spatiotemporal control for the knockdown of target proteins. Here, we present a protocol for AID-mediated protein knockdown in Caenorhabditis elegans. We describe steps for generating the
Externí odkaz:
https://doaj.org/article/70ae19c480524bf5b59f613614852e80
Autor:
Ardalan Hendi, Long-Gang Niu, Andrew William Snow, Richard Ikegami, Zhao-Wen Wang, Kota Mizumoto
Publikováno v:
eLife, Vol 11 (2022)
Precise synaptic connection of neurons with their targets is essential for the proper functioning of the nervous system. A plethora of signaling pathways act in concert to mediate the precise spatial arrangement of synaptic connections. Here we show
Externí odkaz:
https://doaj.org/article/08191d34504c467d92bd3aa4f39ff40b
Publikováno v:
eLife, Vol 10 (2021)
Neuronal cell fate determinants establish the identities of neurons by controlling gene expression to regulate neuronal morphology and synaptic connectivity. However, it is not understood if neuronal cell fate determinants have postmitotic functions
Externí odkaz:
https://doaj.org/article/cf081e50c4a3401fa501aaec6448d877
Autor:
Menghao Lu, Kota Mizumoto
Publikováno v:
eLife, Vol 8 (2019)
During development, the nervous system undergoes a refinement process by which neurons initially extend an excess number of neurites, the majority of which will be eliminated by the mechanism called neurite pruning. Some neurites undergo stereotyped
Externí odkaz:
https://doaj.org/article/d8ff503bf2ee4db3a3cc0d2b6bbb0817
Autor:
Troy A. McDiarmid, Vinci Au, Aaron D. Loewen, Joseph Liang, Kota Mizumoto, Donald G. Moerman, Catharine H. Rankin
Publikováno v:
Disease Models & Mechanisms, Vol 11, Iss 12 (2018)
Our ability to sequence genomes has vastly surpassed our ability to interpret the genetic variation we discover. This presents a major challenge in the clinical setting, where the recent application of whole-exome and whole-genome sequencing has unco
Externí odkaz:
https://doaj.org/article/4d19cfd57e27424a95ab8cf6388ff215
Autor:
Yusuke Miyazaki, Kota Mizumoto, Gautam Dey, Takamasa Kudo, John Perrino, Ling-chun Chen, Tobias Meyer, Thomas J. Wandless
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
Protein aggregates are associated with a wide variety of diseases. Here, in order to address how protein aggregation affects cellular homoeostasis, the authors describe a method to rapidly create protein aggregates in living cells and organisms with
Externí odkaz:
https://doaj.org/article/bd34eaa0d4884eaaa320017ac28b1ea0
Autor:
Xi Chen, Akihiro CE Shibata, Ardalan Hendi, Mizuki Kurashina, Ethan Fortes, Nicholas L Weilinger, Brian A MacVicar, Hideji Murakoshi, Kota Mizumoto
Publikováno v:
eLife, Vol 7 (2018)
During development, neurons form synapses with their fate-determined targets. While we begin to elucidate the mechanisms by which extracellular ligand-receptor interactions enhance synapse specificity by inhibiting synaptogenesis, our knowledge about
Externí odkaz:
https://doaj.org/article/0faa326f7fa0487893d4bcee6e9a28b0
Autor:
Paschalis Kratsios, Sze Yen Kerk, Catarina Catela, Joseph Liang, Berta Vidal, Emily A Bayer, Weidong Feng, Estanisla Daniel De La Cruz, Laura Croci, G Giacomo Consalez, Kota Mizumoto, Oliver Hobert
Publikováno v:
eLife, Vol 6 (2017)
A core principle of nervous system organization is the diversification of neuron classes into subclasses that share large sets of features but differ in select traits. We describe here a molecular mechanism necessary for motor neurons to acquire subc
Externí odkaz:
https://doaj.org/article/cb4afa459544448db13762abc4f8ac2a
Autor:
Kota Mizumoto, Kang Shen
Publikováno v:
Cell Reports, Vol 5, Iss 2, Pp 389-396 (2013)
Gradients of topographic cues play essential roles in the organization of sensory systems by guiding axonal growth cones. Little is known about whether there are additional mechanisms for precise topographic mapping of synaptic connections. Whereas t
Externí odkaz:
https://doaj.org/article/0e02b4b46a094fa6b5a848cd42b854f9
Publikováno v:
Genetics. 223
The nematode Caenorhabditis elegans is a research model organism particularly suited to the mechanistic understanding of synapse genesis in the nervous system. Armed with powerful genetics, knowledge of complete connectomics, and modern genomics, stu