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Autor:
Pérez-Pérez, W. D., Carrasco-Navarro, U., García‑Estrada, C., Kosalková, K., Gutiérrez-Ruíz, M. C., Barrios-González, J., Fierro, F.
Additional file 3. Confirmation of the presence of plasmids for RNAi-mediated silencing of Pc-yap1 and Pc-rsmA and analysis of silencing in P. chrysogenum transformants. (A) Close-up of the pGpdPki-RNAi vector region with the opposite-oriented gpd an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07f9ca2600b162f9c3b71dbbb788cadc
Autor:
Pérez-Pérez, W. D., Carrasco-Navarro, U., García‑Estrada, C., Kosalková, K., Gutiérrez-Ruíz, M. C., Barrios-González, J., Fierro, F.
Additional file 2. Recombinant PCR for overexpression of genes Pc-yap1 and Pc-rsmA. The pki gene promoter from A. niger was fused to fragments from the genes extending from the ATG start codon to around 300 bp downstream the TGA stop codon to ensure
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b458eb33805e555d6eada456dc1b5d93
Autor:
Pérez-Pérez, W. D., Carrasco-Navarro, U., García‑Estrada, C., Kosalková, K., Gutiérrez-Ruíz, M. C., Barrios-González, J., Fierro, F.
Additional file 8. Clustal W alignment of PcYap1 with S. cerevisiae Yap1 and S. pombe Pap1, and conservation of functional domains between them. Nuclear import and export sequences (NLS and NES) are highly conserved. Also conserved are the cysteine-r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24198b58905f4e96cf945cc6e8cd21f2
Autor:
Pérez-Pérez, W. D., Carrasco-Navarro, U., García‑Estrada, C., Kosalková, K., Gutiérrez-Ruíz, M. C., Barrios-González, J., Fierro, F.
Additional file 7. EMSA to analyze possible interactions between PcYap1 and PcRsmA upon binding to their respective binding sites. Probes containing either the PcYap1-binding site (PTA1-WT), the PcRsmA binding site (RsmA-2C) or both (upPta1) were inc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::096c84990b3c0a4960585ae767b35d35
Autor:
Pérez-Pérez, W. D., Carrasco-Navarro, U., García‑Estrada, C., Kosalková, K., Gutiérrez-Ruíz, M. C., Barrios-González, J., Fierro, F.
Additional file 1. Strategy for silencing of expression of Pc-yap1 and Pc-rsmA. (A) A DNA fragment of 458 bp from the exon 2 of Pc-yap1 was amplified by PCR with primers siYAP1-F and -R, digested with NcoI and inserted at the NcoI site of plasmid pGp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c4e5d2fd3dc4d5ef6b041a360c5cb00
Autor:
Pérez-Pérez, W. D., Carrasco-Navarro, U., García‑Estrada, C., Kosalková, K., Gutiérrez-Ruíz, M. C., Barrios-González, J., Fierro, F.
Additional file 5. Isolation of Pc-yap1, Pc-rsmA and Pc-atf21 CDS and insertion in expression vectors for P. pastoris. (A) An RT-PCR was performed using RNA from a submerged culture and primers Yap-1 and Yap-6, which contain restriction sites for Kpn
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f2621c91c4bfd067ba3ee6566e97ed3
Autor:
Pérez-Pérez, W. D., Carrasco-Navarro, U., García‑Estrada, C., Kosalková, K., Gutiérrez-Ruíz, M. C., Barrios-González, J., Fierro, F.
Additional file 6. Purification of recombinant c-myc-6xHis-tagged PcYap1, PcRsmA and PcAtf21 proteins after expression in P. pastoris. Protein extracts were obtained as described in Materials and Methods and loaded onto Ni-NTA Spin 50 (Qiagen) column
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17cadbd846bf19d47d3e2a9a876380c4
Autor:
Pérez-Pérez, W. D., Carrasco-Navarro, U., García‑Estrada, C., Kosalková, K., Gutiérrez-Ruíz, M. C., Barrios-González, J., Fierro, F.
Additional file 4. Confirmation of the presence of plasmids for overexpression of Pc-yap1 and Pc-rsmA in P. chrysogenum transformants. (A) Scheme of the fusion of the pki promoter with the Pc-yap1 (top) and Pc-rsmA (down) genes at the ATG start codon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::967ad2989a54478e0b3fe62f4ca2cc85
Autor:
Pérez-Pérez, W. D., Carrasco-Navarro, U., García‑Estrada, C., Kosalková, K., Gutiérrez-Ruíz, M. C., Barrios-González, J., Fierro, F.
Additional file 9. Clustal W alignment of PcRsmA with A. nidulans RsmA, C. albicans FCR3, and S. cerevisiae Yap3. An NLS is well conserved in all proteins at the same position as in the Yap1 proteins (the beginning of the bZIP domain). Three putative
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82a2f3547b1607013a2fad92e6f3e143