Optimization of a T7-RNA polymerase system in Synechococcus sp. PCC 7002 mirrors the protein overproduction phenotype from E. coli BL21(DE3).

Autor: Jones CM; Chemical Engineering, School for Engineering Matter, Transport, and Energy, Arizona State University, Tempe, AZ, 85287, USA., Korosh TC; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA., Nielsen DR; Chemical Engineering, School for Engineering Matter, Transport, and Energy, Arizona State University, Tempe, AZ, 85287, USA., Pfleger BF; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. pfleger@engr.wisc.edu.

Publikováno v: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2021 Feb; Vol. 105 (3), pp. 1147-1158. Date of Electronic Publication: 2021 Jan 14.

Light-optimized growth of cyanobacterial cultures: Growth phases and productivity of biomass and secreted molecules in light-limited batch growth.

Autor: Clark RL; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, 1415 Engineering Dr., Madison, WI 53706, United States. Electronic address: rlclark2@wisc.edu., McGinley LL; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, 1415 Engineering Dr., Madison, WI 53706, United States. Electronic address: lmcginley@wisc.edu., Purdy HM; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, 1415 Engineering Dr., Madison, WI 53706, United States. Electronic address: hmpurdy@wisc.edu., Korosh TC; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, 1415 Engineering Dr., Madison, WI 53706, United States; Department of Environmental Chemistry and Technology, University of Wisconsin - Madison, 660 N Park St., Madison, WI 53706, United States. Electronic address: korosh@wisc.edu., Reed JL; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, 1415 Engineering Dr., Madison, WI 53706, United States. Electronic address: jlreed@wisc.edu., Root TW; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, 1415 Engineering Dr., Madison, WI 53706, United States. Electronic address: twroot@wisc.edu., Pfleger BF; Department of Chemical and Biological Engineering, University of Wisconsin - Madison, 1415 Engineering Dr., Madison, WI 53706, United States. Electronic address: brian.pfleger@wisc.edu.

Publikováno v: Metabolic engineering [Metab Eng] 2018 May; Vol. 47, pp. 230-242. Date of Electronic Publication: 2018 Mar 27.

Engineering photosynthetic production of L-lysine.

Autor: Korosh TC; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States; Environmental Chemistry and Technology Program, University of Wisconsin-Madison, Madison, WI 53706, United States., Markley AL; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States., Clark RL; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States., McGinley LL; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States., McMahon KD; Environmental Chemistry and Technology Program, University of Wisconsin-Madison, Madison, WI 53706, United States; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI 53706, United States; Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, United States., Pfleger BF; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, United States; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: pfleger@engr.wisc.edu.

Publikováno v: Metabolic engineering [Metab Eng] 2017 Nov; Vol. 44, pp. 273-283. Date of Electronic Publication: 2017 Oct 28.

CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002.

Autor: Gordon GC; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, United States; Microbiology Doctoral Training Program, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI 53706, United States., Korosh TC; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, United States; Department of Environmental Chemistry and Technology, University of Wisconsin-Madison, 660 N Park St, Madison, WI 53706, United States., Cameron JC; Departmentof Chemistry and Biochemistry, University of Colorado-Boulder, 596 UCB, Boulder, CO 80309, United States; Renewable and Sustainable Energy Institute, University of Colorado-Boulder, 027 UCB, Boulder, CO 80309, United States., Markley AL; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, United States., Begemann MB; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, United States; Microbiology Doctoral Training Program, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI 53706, United States., Pfleger BF; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, United States; Microbiology Doctoral Training Program, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI 53706, United States. Electronic address: pfleger@engr.wisc.edu.

Publikováno v: Metabolic engineering [Metab Eng] 2016 Nov; Vol. 38, pp. 170-179. Date of Electronic Publication: 2016 Jul 29.