A Critical Study on DNA Probes Attached to Microplate for CRISPR/Cas12 Trans-Cleavage Activity

Autor: Konstantin M. Burkin, Aleksandr V. Ivanov, Anatoly V. Zherdev, Boris B. Dzantiev, Irina V. Safenkova

Publikováno v: Biosensors, Vol 13, Iss 8, p 824 (2023)

CRISPR/Cas12-based biosensors are emerging tools for diagnostics. However, their application of heterogeneous formats needs the efficient detection of Cas12 activity. We investigated DNA probes attached to the microplate surface and cleaved by Cas12a

Externí odkaz: https://doaj.org/article/738e691859c144ec930d800a47a214a2

One-pot microplate-based chemiluminescent assay coupled with catalytic hairpin assembly amplification for DNA detection

Autor: Oleg L. Bodulev, Eugene E Efremov, Ivan Yu. Sakharov, Konstantin M. Burkin

Publikováno v: Analytical and Bioanalytical Chemistry. 412:5105-5111

Nowadays, considerable efforts are focused on advancing DNA detection methods, which are extremely important in clinical diagnostics, pathogen determination, gene therapy, and forensic analysis. A one-pot sensitive microplate-based chemiluminescent a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1220568cfff98fca0e0f199f5176b58d
https://doi.org/10.1007/s00216-020-02438-6

Comparative study of magnetic beads and microplates as supports in heterogeneous amplified assay of miRNA-141 by using mismatched catalytic hairpin assembly reaction

Autor: Irina V. Safenkova, Konstantin M. Burkin, Oleg L. Bodulev, Shyatesa C. Razo, Aleksandr V. Ivanov, Anatoly V. Zherdev, Boris B. Dzantiev, Ivan Yu Sakharov

Publikováno v: Talanta. 247:123535

Magnetic beads (MBs) are often considered as an effective carrier in heterogeneous assays due to the simplicity of separation and washing, and the ability to increase and control the surface area. However, the effect of the MBs surface on the analyti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30237b1123ec9c03c4139f50597783e1
https://doi.org/10.1016/j.talanta.2022.123535

The Efficiency of Color Space Channels to Quantify Color and Color Intensity Change in Liquids, pH Strips, and Lateral Flow Assays with Smartphones

Autor: Laszlo Bura, Christopher T. Elliott, Katrina Campbell, Konstantin M. Burkin, Karen Rafferty, Yunfeng Zhao, Joost Nelis

Publikováno v: Sensors (Basel, Switzerland)
Sensors
Volume 19
Issue 23
Nelis, J L D, Bura, L, Zhao, Y, Burkin, K M, Rafferty, K, Elliott, C T & Campbell, K 2019, ' The efficiency of color space channels to quantify color and color intensity change in liquids, pH strips, and lateral flow assays with smartphones ', Sensors (Switzerland), vol. 19, no. 23, 5104 . https://doi.org/10.3390/s19235104
Sensors, Vol 19, Iss 23, p 5104 (2019)

Bottom-up, end-user based feed, and food analysis through smartphone quantification of lateral flow assays (LFA) has the potential to cause a paradigm shift in testing capabilities. However, most developed devices do not test the presence of and impl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d9f43b370f839f89bdefd1d369def79
http://europepmc.org/articles/PMC6928750