Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Konstantin Kuznedelov"'
Autor:
Leena Putzeys, Maarten Boon, Eveline-Marie Lammens, Konstantin Kuznedelov, Konstantin Severinov, Rob Lavigne
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 20, Iss , Pp 2624-2638 (2022)
RNA sequencing has become the method of choice to study the transcriptional landscape of phage-infected bacteria. However, short-read RNA sequencing approaches generally fail to capture the primary 5′ and 3′ boundaries of transcripts, confounding
Externí odkaz:
https://doaj.org/article/a67edec9467e43d1a50baf29da314c32
Autor:
Vladimir N Kolyadko, Sofya V Lushchekina, Tatiana A Vuimo, Stepan S Surov, Ruzanna A Ovsepyan, Vera A Korneeva, Ivan I Vorobiev, Nadezhda A Orlova, Leonid Minakhin, Konstantin Kuznedelov, Konstantin V Severinov, Fazoil I Ataullakhanov, Mikhail A Panteleev
Publikováno v:
PLoS ONE, Vol 10, Iss 12, p e0144940 (2015)
Factor XIIa (fXIIa) is a serine protease that triggers the coagulation contact pathway and plays a role in thrombosis. Because it interferes with coagulation testing, the need to inhibit fXIIa exists in many cases. Infestin-4 (Inf4) is a Kazal-type i
Externí odkaz:
https://doaj.org/article/a3e33141cf504a309b6f154e7fb7c5c9
Autor:
Anna A. Shiriaeva, Konstantin Kuznedelov, Ivan Fedorov, Olga Musharova, Timofey Khvostikov, Yuliya Tsoy, Elena Kurilovich, Gerald R. Smith, Ekaterina Semenova, Konstantin Severinov
Publikováno v:
Science Advances. 8
CRISPR-Cas systems provide prokaryotes with adaptive immunity against foreign nucleic acids. In Escherichia coli , immunity is acquired upon integration of 33-bp spacers into CRISPR arrays. DNA targets complementary to spacers get degraded and serve
Autor:
Konstantin Kuznedelov, Natalia Morozova, Sofya Medvedeva, Alexandr Kirillov, Konstantin Severinov, Eugene V. Koonin, Matvey Kolesnik, Leonid Minakhin, Ekaterina Semenova, Anna Shiriaeva, Kira S. Makarova, Sergei Borukhov, Ishita Jain
Type VI CRISPR-Cas systems are the only CRISPR variety that cleaves exclusively RNA1,2. In addition to the CRISPR RNA (crRNA)-guided, sequence-specific binding and cleavage of target RNAs, such as phage transcripts, the type VI effector, Cas13, cause
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b53841ca3340abaf0b47fa866ecf57b9
https://doi.org/10.21203/rs.3.rs-1068910/v1
https://doi.org/10.21203/rs.3.rs-1068910/v1
Autor:
Regina Shebanova, Natalia Nikitchina, Nikita Shebanov, Vladimir Mekler, Konstantin Kuznedelov, Egor Ulashchik, Ruslan Vasilev, Olga Sharko, Vadim Shmanai, Ivan Tarassov, Konstantin Severinov, Nina Entelis, Ilya Mazunin
Publikováno v:
Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2021, ⟨10.1093/nar/gkab1227⟩
Nucleic Acids Research, Oxford University Press, 2021, ⟨10.1093/nar/gkab1227⟩
CRISPR RNAs (crRNAs) that direct target DNA cleavage by Type V Cas12a nucleases consist of constant repeat-derived 5′-scaffold moiety and variable 3′-spacer moieties. Here, we demonstrate that removal of most of the 20-nucleotide scaffold has onl
Autor:
Ishita Jain, Matvey Kolesnik, Leonid Minakhin, Natalia Morozova, Anna Shiriaeva, Alexandr Kirillov, Sofia Medvedeva, Konstantin Kuznedelov, Sergei Borukhov, Kira S. Makarova, Eugene V. Koonin, Konstantin Severinov, Ekaterina Semenova
Type VI CRISPR-Cas systems are the only CRISPR variety that cleaves exclusively RNA1,2. In addition to the CRISPR RNA (crRNA)-guided, sequence-specific binding and cleavage of target RNAs, such as phage transcripts, the type VI effector, Cas13, cause
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3c369fce9b3a57b7a278a411fcac11a8
https://doi.org/10.1101/2021.11.10.468108
https://doi.org/10.1101/2021.11.10.468108
Autor:
T. O. Artamonova, Anatolii Arseniev, Konstantin Kuznedelov, Ruslan Kondratenko, Leah B. Bushin, Mikhail Metelev, Mohammad R. Seyedsayamdost, Konstantin Severinov, Mikhail Khodorkovskii
Publikováno v:
ACS Chemical Biology. 12:814-824
We report the bioinformatic prediction and structural validation of two lasso peptides, acinetodin and klebsidin, encoded by the genomes of several human-associated strains of Acinetobacter and Klebsiella. Computation of the three-dimensional structu
Autor:
Scott Bailey, Sergey Shmakov, Ekaterina Savitskaya, John Mallon, Kirill A. Datsenko, Konstantin Kuznedelov, Ishita Jain, Brian Bothner, Alexander F. Yakunin, Konstantin Severinov, Ekaterina Semenova, Monika Tokmina-Lukaszewska, Vladimir Mekler, Sofia Lemak
Publikováno v:
Nucleic Acids Research
The Escherichia coli type I-E CRISPR-Cas system Cascade effector is a multisubunit complex that binds CRISPR RNA (crRNA). Through its 32-nucleotide spacer sequence, Cascade-bound crRNA recognizes protospacers in foreign DNA, causing its destruction d
Autor:
Konstantin Kuznedelov, Ekaterina Semenova, Leonid Minakhin, Vladimir Mekler, Konstantin Severinov
Publikováno v:
Nucleic Acids Research
CRISPR-Cas9 is widely applied for genome engineering in various organisms. The assembly of single guide RNA (sgRNA) with the Cas9 protein may limit the Cas9/sgRNA effector complex function. We developed a FRET-based assay for detection of CRISPR–Ca
Autor:
Ralf Seidel, Olga Musharova, Marius Rutkauskas, Konstantin Kuznedelov, Andrey Krivoy, Christophe Rouillon, Konstantin Severinov
Publikováno v:
Nucleic Acids Research
Skoltech Ph.D. program in the Life Sciences (to A.K.); European Research Council consolidator grant [GA 724863 to R.S].; NIH [R01 GM10407] and Russian Science Foundation [14-14-00988] grants to K.S.; UMNIK grant 8115GU/2015 to O.M., and institutional
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47a9cc4d78d40318fdb96de649307678
https://hdl.handle.net/10023/13172
https://hdl.handle.net/10023/13172