Zobrazeno 1 - 10
of 222
pro vyhledávání: '"Konstantin A. Lukyanov"'
Autor:
Elena L. Sokolinskaya, Olga N. Ivanova, Irina T. Fedyakina, Alexander V. Ivanov, Konstantin A. Lukyanov
Publikováno v:
International Journal of Molecular Sciences, Vol 25, Iss 12, p 6635 (2024)
Papain-like protease PLpro, a domain within a large polyfunctional protein, nsp3, plays key roles in the life cycle of SARS-CoV-2, being responsible for the first events of cleavage of a polyprotein into individual proteins (nsp1–4) as well as for
Externí odkaz:
https://doaj.org/article/780256748d5b41fbbfd2ffbe5684a100
Insight into redox regulation of apoptosis in cancer cells with multiparametric live-cell microscopy
Autor:
Marina V. Shirmanova, Alena I. Gavrina, Tatiana F. Kovaleva, Varvara V. Dudenkova, Ekaterina E. Zelenova, Vladislav I. Shcheslavskiy, Artem M. Mozherov, Ludmila B. Snopova, Konstantin A. Lukyanov, Elena V. Zagaynova
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022)
Abstract Cellular redox status and the level of reactive oxygen species (ROS) are important regulators of apoptotic potential, playing a crucial role in the growth of cancer cell and their resistance to apoptosis. However, the relationships between t
Externí odkaz:
https://doaj.org/article/27233228ceec49c6b81372010e3c287e
Amino acid residue at the 165th position tunes EYFP chromophore maturation. A structure-based design
Autor:
Nadya V. Pletneva, Eugene G. Maksimov, Elena A. Protasova, Anastasia V. Mamontova, Tatiana R. Simonyan, Rustam H. Ziganshin, Konstantin A. Lukyanov, Liya Muslinkina, Sergei Pletnev, Alexey M. Bogdanov, Vladimir Z. Pletnev
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 19, Iss , Pp 2950-2959 (2021)
For the whole GFP family, a few cases, when a single mutation in the chromophore environment strongly inhibits maturation, were described. Here we study EYFP-F165G – a variant of the enhanced yellow fluorescent protein – obtained by a single F165
Externí odkaz:
https://doaj.org/article/4132c07af9ba4fe2948940946344ab2f
Autor:
Nina G. Bozhanova, Joel M. Harp, Brian J. Bender, Alexey S. Gavrikov, Dmitry A. Gorbachev, Mikhail S. Baranov, Christina B. Mercado, Xuan Zhang, Konstantin A. Lukyanov, Alexander S. Mishin, Jens Meiler
Publikováno v:
PLoS Computational Biology, Vol 17, Iss 11 (2021)
The use of unnatural fluorogenic molecules widely expands the pallet of available genetically encoded fluorescent imaging tools through the design of fluorogen activating proteins (FAPs). While there is already a handful of such probes available, eac
Externí odkaz:
https://doaj.org/article/826bc1b7f0f148b8ad96f37c2d2ca4db
Autor:
Marina V. Shirmanova, Alena I. Gavrina, Tatiana F. Kovaleva, Varvara V. Dudenkova, Ekaterina E. Zelenova, Vladislav I. Shcheslavskiy, Artem M. Mozherov, Ludmila B. Snopova, Konstantin A. Lukyanov, Elena V. Zagaynova
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-2 (2022)
Externí odkaz:
https://doaj.org/article/8c59537e6f5b4e128739f2185f5168b2
Autor:
Tatiana R. Simonyan, Elena A. Protasova, Anastasia V. Mamontova, Aleksander M. Shakhov, Konstantin A. Lukyanov, Eugene G. Maksimov, Alexey M. Bogdanov
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 21, p 12907 (2022)
The real-time monitoring of the intracellular pH in live cells with high precision represents an important methodological challenge. Although genetically encoded fluorescent indicators can be considered as a probe of choice for such measurements, the
Externí odkaz:
https://doaj.org/article/46d4dc7837f84fdbba3e1d839570f341
Autor:
Afanasii I. Stepanov, Zlata V. Besedovskaia, Maria A. Moshareva, Konstantin A. Lukyanov, Lidia V. Putlyaeva
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 16, p 8988 (2022)
Epigenetic modifications of histones (methylation, acetylation, phosphorylation, etc.) are of great importance in determining the functional state of chromatin. Changes in epigenome underlay all basic biological processes, such as cell division, diff
Externí odkaz:
https://doaj.org/article/ef09b3924831426d94397245bb85f60d
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 14, p 7826 (2022)
In the SARS-CoV-2 lifecycle, papain-like protease PLpro cuts off the non-structural proteins nsp1, nsp2, and nsp3 from a large polyprotein. This is the earliest viral enzymatic activity, which is crucial for all downstream steps. Here, we designed tw
Externí odkaz:
https://doaj.org/article/08fcac5a4a2e41338f4412604014db9e
Autor:
Dmitry A. Gorbachev, Elizaveta F. Petrusevich, Adil M. Kabylda, Eugene G. Maksimov, Konstantin A. Lukyanov, Alexey M. Bogdanov, Mikhail S. Baranov, Anastasia V. Bochenkova, Alexander S. Mishin
Publikováno v:
Frontiers in Molecular Biosciences, Vol 7 (2020)
Here we dissect the phenomena of oxidative and reductive green-to-red photoconversion of the Green Fluorescent Protein. We characterize distinct orange- and red-emitting forms (λabs/λem = 490/565 nm; λabs/λem = 535/600 nm) arising during the Enha
Externí odkaz:
https://doaj.org/article/3c47c196c9fb4874b5aa3646700a21ad
Publikováno v:
International Journal of Molecular Sciences, Vol 22, Iss 21, p 11799 (2021)
Fluorescent labeling is an established method for visualizing cellular structures and dynamics. The fundamental diffraction limit in image resolution was recently bypassed with the development of super-resolution microscopy. Notably, both localizatio
Externí odkaz:
https://doaj.org/article/c23debb0f7f54e0babac0704da2cc759