Regulation of Constitutive GPR3 Signaling and Surface Localization by GRK2 and β-arrestin-2 Overexpression in HEK293 Cells.

Autor: Katie M Lowther, Tracy F Uliasz, Konrad R Götz, Viacheslav O Nikolaev, Lisa M Mehlmann

Publikováno v: PLoS ONE, Vol 8, Iss 6, p e65365 (2013)

G protein-coupled receptor 3 (GPR3) is a constitutively active receptor that maintains high 3'-5'-cyclic adenosine monophosphate (cAMP) levels required for meiotic arrest in oocytes and CNS function. Ligand-activated G protein-coupled receptors (GPCR

Externí odkaz: https://doaj.org/article/065e640df7de4ffa8323c5ba38a05418

Enhanced expression of β3-adrenoceptors in cardiac myocytes attenuates neurohormone-induced hypertrophic remodeling through nitric oxide synthase

Autor: Irina Lobysheva, Dominique Langin, Jean-Luc Balligand, A.C. Pouleur, Annelies Vanderper, Konrad R. Götz, Karima Jnaoui, Christophe Beauloye, Denise Hilfiker-Kleiner, Andreas Markl, Hrag Esfahani, Emilie Dubois-Deruy, Luc Bertrand, Guido Iaccarino, Joanna Hammond, Geneviève Tavernier, Paul Herijgers, Viacheslav O. Nikolaev, Julien Hamelet, Catharina Belge, Chantal Dessy, Boris Manoury

Publikováno v: Circulation
Circulation, American Heart Association, 2014, 129 (4), pp.451-62. ⟨10.1161/CIRCULATIONAHA.113.004940⟩
Circulation, 2014, 129 (4), pp.451-62. ⟨10.1161/CIRCULATIONAHA.113.004940⟩

Background— β1-2-adrenergic receptors (AR) are key regulators of cardiac contractility and remodeling in response to catecholamines. β3-AR expression is enhanced in diseased human myocardium, but its impact on remodeling is unknown. Methods and R

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1b2133680b2047560dd19e418778ffa
http://hdl.handle.net/11588/835615

Advances and Techniques to Measure cGMP in Intact Cardiomyocytes

Autor: Viacheslav O. Nikolaev, Konrad R. Götz

Publikováno v: Methods in Molecular Biology ISBN: 9781627034586

Forster resonance energy transfer (FRET)-based biosensors are powerful tools for real-time monitoring of signaling events in intact cells using fluorescence microscopy. Here, we describe a highly sensitive method which allows FRET-based measurements

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9df6f9cda781ff7fdad520b351d2a72f
https://doi.org/10.1007/978-1-62703-459-3_7

290β-3 adrenoreceptors protect from hypertrophic remodelling through nitric oxide synthase & AMP-activated protein kinase dependent signaling pathways

Autor: J Hammond, E Dubois-Deruy, B. Manoury, J.L. Balligand, Chantal Dessy, Luc Bertrand, Viacheslav O. Nikolaev, Konrad R. Götz, H Esfahani, R. Gelinas

Publikováno v: Cardiovascular Research. 103:S53.1-S53

Background: Moderate overexpression of β3AR in cardiomyocytes attenuates hypertrophic remodeling in hearts after neurohormonal stress, without impairement of cardiac function. Methods: Functional analyses were carried out in wild type (WT) and heter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::db78eb6d0ce50c97e26c9087f2182dff
https://doi.org/10.1093/cvr/cvu087.4

Direct visualization of cGMP microdomains in adult cardiomyocytes

Autor: Konrad R. Götz, Hariharan Subramanian, Daniela Hübscher, Alexander Froese, Viacheslav O. Nikolaev

Publikováno v: BMC Pharmacology & Toxicology

Results Cardiomyocytes were isolated from the transgenic mice expressing the cytosolic red cGES-DE5 sensor [1]. Using the previously established scanning ion conductance microscopy (SICM)/FRET method [2], local application of Atrial Natriuretic Pepti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f4ee1b369b1ff02cd7dda26d593a785