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Detection of Streptococcus mutans by PCR amplification of spaP gene

Autor: E. J. Fernandez, Komei Fukui, K Hirota, K. Nemoto, Takahito Ono, Fusao Ota

Publikováno v: Journal of Medical Microbiology. 41:231-235

Synthetic oligonucleotide primers were used in the polymerase chain reaction (PCR) to amplify a sequence of the spaP gene, which encodes the surface protein antigen I/II of Streptococcus mutans. A DNA fragment of c. 192 bp was amplified from lysed S.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f818b17037e4e1963be6f59bfcffa37b
https://doi.org/10.1099/00222615-41-4-231

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Experimental glomerulonephritis caused by Streptococcus mutans

Autor: Yuji Morimoto, Jin Zhan Rui, Komei Fukui, Kaname Okada, Yasuhiro Kuroda, K Hirota, Fusao Ota, Shoji Kagami

Publikováno v: Nihon Shoni Jinzobyo Gakkai Zasshi. 7:204-207

Streptococcus mutans (S. mutans) をラットに1回静注し，増殖性糸球体腎炎を惹起することができた。 S. pyogenesと共通する多糖体抗原に対する抗体の存在下ではより強い腎病変が認められた。腎炎

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0d46e75e64c6934241f94e19c50e07c1
https://doi.org/10.3165/jjpn.7.204

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Effects of protein intake on immune parameters in mice surviving streptococcal infection

Autor: Katsuhiko Hirota, Shigeru Yamamoto, Komei Fukui, Kyoichi Kishi, Fusao Ota, Yasuhiro Kido, Sayoko Takeuchi, Miyuki Akiyama

Publikováno v: FEMS Microbiology Letters. 89:111-122

Groups of mice were fed for 2 weeks on isocaloric diets containing 5, 7, 20 and 40% (w/w) casein, respectively, then injected intraperitoneally with group B streptococci, and observed for their survival rates. The mice fed 7% or 20% casein had lower

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c71ae0bcbb043279d512158af3324b0d
https://doi.org/10.1111/j.1574-6968.1992.tb04977.x

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Cross-reactive Polysaccharide Antigens (Types a, d, and h) of the Mutans Group of Streptococci: Different Molecular Forms of the Type as Distinguishable by Monoclonal Antibodies

Autor: Hideaki Nagamune, Yasuyuki Akiyama, Yoshihiro Minato, Komei Fukui, Fusao Ota, T Koga, Kuniko Seo, Hirohisa Kato

Publikováno v: Zentralblatt für Bakteriologie. 272:294-305

As compared to the previous precipitin inhibition tests differences were found in thereactivities of monoclonal antibodies (MAbs), a-4 and a-84 with Streptococcus cricetus (serotype a) in an enzyme immunoassay using whole cells, purified cell wall an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a73efeb9bb78ef5aa093dd7e2dada7b7
https://doi.org/10.1016/s0934-8840(11)80031-9

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Type-Specific Antigen of Streptococcus rattus Strains (KAY1 and FA1). I. Characterization

Autor: Yoshihiro Minato, Hirohisa Kato, Miwako Ono, Yoshio Takeda, Yasuyori Morimoto, Fusao Ota, Yasuyuki Akiyama, Komei Fukui, Yasuhiko Yasuoka, Hideaki Nagamune

Publikováno v: Zentralblatt für Bakteriologie. 271:36-45

A serotype-specific antigen was extracted from Streptococcus rattus KAY1 strain isolated first in Japan from human dental plaque and purified on an ion exchange column to compare it chemically and immunologically with that of FA1 strain which had bee

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b1a9534b6620645080f670d4cc881d1
https://doi.org/10.1016/s0934-8840(89)80051-9

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Serological typing of reference strains and clinical isolates of streptococcus mutans by agglutination reactions and/or radioimmunoassay

Autor: Masahito Yasuoka, Fusao Ota, Komei Fukui, Yasuyuki Akiyama, Yasuhiko Satomi, Michiko Kiso

Publikováno v: Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology. 265:330-339

Rabbit antisera were prepared against eight different serotypes of Streptococcus mutans. After absorption with heterologous serotype strains the antisera were able to distinguish 21 reference strains of different serotypes of S. mutans by simple aggl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae9a487723573a60f012fdce04897021
https://doi.org/10.1016/s0176-6724(87)80251-1

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Directional immobilization of sodium- and potassium-activated ATPase to expose its cytoplasmic part to the liquid phase on microtiter plates by wheat germ agglutinin

Autor: Katsuhiko Hirota, K Seo, Hideaki Nagamune, Komei Fukui, M Nakao, Yasuhiko Satomi, O Urayama, Fusao Ota, Y Hara

Publikováno v: Analytical Biochemistry. 180:362-367

A convenient method for highly efficient and directional immobilization of intact sodium- and potassium-activated ATPase (Na,K-ATPase) using wheat germ agglutinin linked on microtiter plates was developed. Wheat germ agglutinin, which bound tightly t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28af7100c77c04646eb1ecdc8045e420
https://doi.org/10.1016/0003-2697(89)90446-6

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A comparison of competitive enzyme immunoassay and precipitin inhibition tests in the analysis of polysaccharide antigenic determinants of oral streptococci

Autor: Yasuhiko Satomi, Katsuhiko Hirota, Fusao Ota, Terushige Kawata, Hideaki Nagamune, Komei Fukui, Masahiko Yasuoka, Sonia Senesi, Yasuyuki Akiyama

Publikováno v: Journal of Immunological Methods. 124:259-266

A rapid and convenient method of competitive enzyme immunoassay (EIA) was explored for the immunological analysis by MAbs of polysaccharide antigens prepared from the mutans group of streptococci, and optimal conditions for it were established. These

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8f85779d927fa10cf8fd2eca1c1cc83
https://doi.org/10.1016/0022-1759(89)90362-1

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