Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin

Autor: Ingrid Chamma, Mathieu Letellier, Corey Butler, Béatrice Tessier, Kok-Hong Lim, Isabel Gauthereau, Daniel Choquet, Jean-Baptiste Sibarita, Sheldon Park, Matthieu Sainlos, Olivier Thoumine

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-15 (2016)

The advent of fluorescence-based super-resolution microscopy has created a need for labeling strategies relying on small probes that minimally perturb protein function. Here the authors describe a labeling method that reduces protein tag and label si

Externí odkaz: https://doaj.org/article/5f33096ea5754f40ac84a9bb09f90a56

Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin

Autor: Mathieu Letellier, Daniel Choquet, Jean-Baptiste Sibarita, Olivier Thoumine, Isabel Gauthereau, Béatrice Tessier, Sheldon Park, Kok Hong Lim, Matthieu Sainlos, Corey Butler, Ingrid Chamma

Publikováno v: Nature Communications
Nature Communications, Nature Publishing Group, 2016, 7 (1), ⟨10.1038/ncomms10773⟩
Nature Communications, Vol 7, Iss 1, Pp 1-15 (2016)

The advent of super-resolution imaging (SRI) has created a need for optimized labelling strategies. We present a new method relying on fluorophore-conjugated monomeric streptavidin (mSA) to label membrane proteins carrying a short, enzymatically biot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fae339f9bb2b3c39a4ff949a5538143
http://europepmc.org/articles/PMC4799371

Engineered Streptavidin Monomer and Dimer with Improved Stability and Function

Autor: Sheldon Park, Heng Huang, Arnd Pralle, Kok Hong Lim

Publikováno v: Biochemistry. 50:8682-8691

Although streptavidin's high affinity for biotin has made it a widely used and studied binding protein and labeling tool, its tetrameric structure may interfere with some assays. A streptavidin mutant with a simpler quaternary structure would demonst

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6ceb8719753bcc5300f081f21330401
https://doi.org/10.1021/bi2010366

Expression of a soluble flavone synthase allows the biosynthesis of phytoestrogen derivatives in Escherichia coli

Autor: Effendi Leonard, Kok Hong Lim, Mattheos A. G. Koffas, Joseph A. Chemler

Publikováno v: Applied Microbiology and Biotechnology. 70:85-91

Flavones are plant secondary metabolites with potent pharmacological properties. We report the functional expression of FSI, a flavonoid 2-oxoglutarate-dependent dioxygenase-encoding flavone synthase from parsley in Escherichia coli. This expression

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c2264975530e379ef4ddba141cf11e0
https://doi.org/10.1007/s00253-005-0059-x

Structure-based engineering of streptavidin monomer with a reduced biotin dissociation rate

Autor: Daniel, Demonte, Eric J, Drake, Kok Hong, Lim, Andrew M, Gulick, Sheldon, Park

Publikováno v: Proteins. 81(9)

We recently reported the engineering of monomeric streptavidin, mSA, corresponding to one subunit of wild type (wt) streptavidin tetramer. The monomer was designed by homology modeling, in which the streptavidin and rhizavidin sequences were combined

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::dd59243b851ff454495616e1640c72fb
https://pubmed.ncbi.nlm.nih.gov/23670729