Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Kohki Kido"'
Autor:
Satoshi Yamanaka, Yuto Horiuchi, Saya Matsuoka, Kohki Kido, Kohei Nishino, Mayaka Maeno, Norio Shibata, Hidetaka Kosako, Tatsuya Sawasaki
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)
PROTACs and molecular glues target E3 ubiquitin ligases to substrate proteins. Here, the authors develop a proximity biotinylation-based method to identify drug-induced E3 ligase-substrate interactions, enabling the assessment of the target spectrum
Externí odkaz:
https://doaj.org/article/5e14510e4bfc4a18b28054cf46f4d314
Autor:
Kohki Kido, Satoshi Yamanaka, Shogo Nakano, Kou Motani, Souta Shinohara, Akira Nozawa, Hidetaka Kosako, Sohei Ito, Tatsuya Sawasaki
Publikováno v:
eLife, Vol 9 (2020)
Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying proteins that interact with a target protein in a cell or organism. However, there have been some improvements in the
Externí odkaz:
https://doaj.org/article/58015ddcf4fb4ccc8dc53d30006056da
Autor:
Hirotaka Takahashi, Satoshi Yamanaka, Shohei Kuwada, Kana Higaki, Kohki Kido, Yusuke Sato, Shuya Fukai, Fuminori Tokunaga, Tatsuya Sawasaki
Publikováno v:
Biomedicines, Vol 8, Iss 6, p 152 (2020)
Protein ubiquitinations play pivotal roles in many cellular processes, including homeostasis, responses to various stimulations, and progression of diseases. Deubiquitinating enzymes (DUBs) remove ubiquitin molecules from ubiquitinated proteins and c
Externí odkaz:
https://doaj.org/article/1275cf15241d427a9a762147d7cf1105
Autor:
Hiroyuki Takeda, Wei Zhou, Kohki Kido, Ryoji Suno, Takahiro Iwasaki, Takuya Kobayashi, Tatsuya Sawasaki
Publikováno v:
PLoS ONE, Vol 12, Iss 5, p e0178246 (2017)
There are many strategies to purify recombinant proteins of interest, and affinity purification utilizing monoclonal antibody that targets a linear epitope sequence is one of the essential techniques used in current biochemistry and structural biolog
Externí odkaz:
https://doaj.org/article/2c7b9dba45b74f52afa3004a8bb1cd37
Autor:
Ryouhei, Shioya, Kohdai, Yamada, Kohki, Kido, Hirotaka, Takahashi, Akira, Nozawa, Hidetaka, Kosako, Tatsuya, Sawasaki
Publikováno v:
Biochemical and Biophysical Research Communications. 592:54-59
Proteins and antibodies labeled with biotin have been widely used for protein analysis, enzyme immunoassays, and diagnoses. Presently, they are prepared using either a chemical reaction involving a biotin N-hydroxysuccinimide (NHS) ester compound or
Autor:
Soh Tokunaga, Miwako Denda, Satoshi Ozawa, Kohki Kido, Ryo Morishita, Shusei Sugiyama, Tatsuya Sawasaki, Ryouhei Shioya
Publikováno v:
Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
Antibodies are widely used for the detection of specific molecules such as peptides, proteins, and chemical compounds. The specificity of an antibody is therefore its most important feature. However, it is very difficult to confirm antibody specifici
Autor:
Kentaro Semba, Hiromi Hiyoshi, Takashi Joh, Eiji Kubota, Masashi Maekawa, Kohki Kido, Tatsuya Sawasaki, Shigeki Higashiyama, Jun Nakayama
Publikováno v:
Biochemical and Biophysical Research Communications. 516:1116-1122
Eukaryotic translation initiation factor 3 subunit D (EIF3D) binds to the 5'-cap of specific mRNAs, initiating their translation into polypeptides. From a pathological standpoint, EIF3D has been observed to be essential for cell growth in various can
Autor:
Akira Nozawa, Kohki Kido, Sohei Ito, Tatsuya Sawasaki, Hidetaka Kosako, Shogo Nakano, Souta Shinohara, Kou Motani, Satoshi Yamanaka
Publikováno v:
eLife
eLife, Vol 9 (2020)
eLife, Vol 9 (2020)
Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying proteins that interact with a target protein in a cell or organism. However, there have been some improvements in the
Autor:
Ryo, Morishita, Shusei, Sugiyama, Miwako, Denda, Soh, Tokunaga, Kohki, Kido, Ryouhei, Shioya, Satoshi, Ozawa, Tatsuya, Sawasaki
Publikováno v:
Scientific Reports
Antibodies are widely used for the detection of specific molecules such as peptides, proteins, and chemical compounds. The specificity of an antibody is therefore its most important feature. However, it is very difficult to confirm antibody specifici