Efficient enzyme-free isolation of brain-derived extracellular vesicles.

Autor: Matamoros-Angles A; Institute of Neuropathology, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Karadjuzovic E; Institute of Neuropathology, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Mohammadi B; Institute of Neuropathology, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Song F; Institute of Neuropathology, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Brenna S; Department of Neurology, Experimental Research in Stroke and Inflammation (ERSI), University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Meister SC; Institute of Neuropathology, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Siebels B; Section Mass Spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Voß H; Section Mass Spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Seuring C; Multi-User-CryoEM-Facility, Centre for Structural Systems Biology (CSSB), Hamburg, Germany.; Department of Chemistry, Universität Hamburg, Hamburg, Germany.; Leibniz Institute of Virology (LIV), Hamburg, Germany., Ferrer I; IDIBELL, University of Barcelona, L'Hospitalet de Llobregat, Spain., Schlüter H; Section Mass Spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Kneussel M; Institute for Molecular Neurogenetics, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Altmeppen HC; Institute of Neuropathology, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Schweizer M; Electron Microscopy Core Facility, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Puig B; Department of Neurology, Experimental Research in Stroke and Inflammation (ERSI), University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Shafiq M; Institute of Neuropathology, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany., Glatzel M; Institute of Neuropathology, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.

Publikováno v: Journal of extracellular vesicles [J Extracell Vesicles] 2024 Nov; Vol. 13 (11), pp. e70011.

The autism susceptibility kinase, TAOK2, phosphorylates eEF2 and modulates translation.

Autor: Henis M; Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.; Department of Anatomy and Embryology, Faculty of Veterinary Medicine, New Valley University, 72511 El-Kharga, Egypt., Rücker T; Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany., Scharrenberg R; Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany., Richter M; Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany., Baltussen L; VIB Center for Brain & Disease Research, Herestraat 49, 3000 Leuven, Belgium.; KU Leuven Department of Neurosciences, Leuven Brain Institute, Herestraat 49, 3000 Leuven, Belgium., Hong S; Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany., Meka DP; Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany., Schwanke B; Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany., Neelagandan N; Neuronal Translational Control Group, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf (UKE), Falkenried 94, 20251 Hamburg, Germany.; Institute of Bioengineering (IBI), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland., Daaboul D; VIB Center for Brain & Disease Research, Herestraat 49, 3000 Leuven, Belgium.; KU Leuven Department of Neurosciences, Leuven Brain Institute, Herestraat 49, 3000 Leuven, Belgium., Murtaza N; Krembil Research Institute, Donald K. Johnson Eye Institute, University Health Network, 60 Leonard Ave, Toronto, Ontario M5T 0S8, Canada.; Department of Biochemistry and Biomedical Sciences, Faculty of Health Sciences, McMaster University, Hamilton, Ontario L8S 4A9, Canada., Krisp C; Institute for Clinical Chemistry and Laboratory Medicine, Mass Spectrometric Proteomics Group, Campus Forschung, University Medical Center Hamburg-Eppendorf (UKE), 20246 Hamburg, Germany., Harder S; Institute for Clinical Chemistry and Laboratory Medicine, Mass Spectrometric Proteomics Group, Campus Forschung, University Medical Center Hamburg-Eppendorf (UKE), 20246 Hamburg, Germany., Schlüter H; Institute for Clinical Chemistry and Laboratory Medicine, Mass Spectrometric Proteomics Group, Campus Forschung, University Medical Center Hamburg-Eppendorf (UKE), 20246 Hamburg, Germany., Kneussel M; Institute of Neurogenetics, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf (UKE), 20251 Hamburg, Germany., Hermans-Borgmeyer I; Transgenic Service Group, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf (UKE), Falkenried 94, 20251 Hamburg, Germany., de Wit J; VIB Center for Brain & Disease Research, Herestraat 49, 3000 Leuven, Belgium.; KU Leuven Department of Neurosciences, Leuven Brain Institute, Herestraat 49, 3000 Leuven, Belgium., Singh KK; Krembil Research Institute, Donald K. Johnson Eye Institute, University Health Network, 60 Leonard Ave, Toronto, Ontario M5T 0S8, Canada.; Faculty of Medicine, University of Toronto, Medical Sciences Building, 1 King's College Cir, Toronto, Ontario M5S 1 A8, Canada., Duncan KE; Neuronal Translational Control Group, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf (UKE), Falkenried 94, 20251 Hamburg, Germany.; Evotec SE, Manfred Eigen Campus, Essener Bogen 7, 22419 Hamburg, Germany., de Anda FC; Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.

Publikováno v: Science advances [Sci Adv] 2024 Apr 12; Vol. 10 (15), pp. eadf7001. Date of Electronic Publication: 2024 Apr 12.

Tubb3 expression levels are sensitive to neuronal activity changes and determine microtubule growth and kinesin-mediated transport.

Autor: Radwitz J; Institute for Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, 20251, Hamburg, Germany., Hausrat TJ; Institute for Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, 20251, Hamburg, Germany., Heisler FF; Institute for Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, 20251, Hamburg, Germany., Janiesch PC; Institute for Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, 20251, Hamburg, Germany., Pechmann Y; Institute for Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, 20251, Hamburg, Germany., Rübhausen M; Institut für Nanostruktur- und Festkörperphysik, Center for Free Electron Laser Science (CFEL), University of Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany., Kneussel M; Institute for Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, Falkenried 94, 20251, Hamburg, Germany. matthias.kneussel@zmnh.uni-hamburg.de.

Publikováno v: Cellular and molecular life sciences : CMLS [Cell Mol Life Sci] 2022 Oct 29; Vol. 79 (11), pp. 575. Date of Electronic Publication: 2022 Oct 29.

Choroid plexuses carry nodal-like cilia that undergo axoneme regression from early adult stage.

Autor: Ho KH; Schaller Research Group, German Cancer Research Center (DKFZ), Heidelberg 69120, Germany; Faculty of Biosciences, Heidelberg University, Heidelberg 69120, Germany., Candat A; Electron Microscopy Facility, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France., Scarpetta V; Schaller Research Group, German Cancer Research Center (DKFZ), Heidelberg 69120, Germany; Department of Neurosciences 'Rita Levi Montalcini,' University of Turin, Turin 10126, Italy., Faucourt M; Cilia biology and Neurogenesis Team, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France., Weill S; Computational Bioimaging and Bioinformatics Team, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France., Salio C; Department of Veterinary Sciences, University of Turin, Grugliasco 10095, Italy., D'Este E; Optical Microscopy Facility, Max Planck Institute for Medical Research, Heidelberg 69120, Germany., Meschkat M; Abberior Instruments GmbH, Göttingen 37077, Germany., Wurm CA; Abberior Instruments GmbH, Göttingen 37077, Germany., Kneussel M; Department of Molecular Neurogenetics, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf, Hamburg 20251, Germany., Janke C; Institut Curie, PSL Research University, CNRS UMR 3348, Orsay 91401, France; Université Paris-Saclay, CNRS UMR 3348, Orsay 91401, France., Magiera MM; Institut Curie, PSL Research University, CNRS UMR 3348, Orsay 91401, France; Université Paris-Saclay, CNRS UMR 3348, Orsay 91401, France., Genovesio A; Computational Bioimaging and Bioinformatics Team, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France., Meunier A; Cilia biology and Neurogenesis Team, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France., Sassoè-Pognetto M; Department of Neurosciences 'Rita Levi Montalcini,' University of Turin, Turin 10126, Italy., Brill MS; Institute of Neuronal Cell Biology, Technical University of Munich, Munich 80802, Germany; Munich Cluster of Systems Neurology (SyNergy), Munich 81377, Germany., Spassky N; Cilia biology and Neurogenesis Team, Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole normale supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France., Patrizi A; Schaller Research Group, German Cancer Research Center (DKFZ), Heidelberg 69120, Germany; Interdisciplinary Center for Neuroscience, Heidelberg University, Heidelberg 69120, Germany; Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance, Heidelberg 69120, Germany. Electronic address: a.patrizi@dkfz.de.

Publikováno v: Developmental cell [Dev Cell] 2023 Dec 04; Vol. 58 (23), pp. 2641-2651.e6. Date of Electronic Publication: 2023 Oct 26.

Tetraspanin 15 depletion impairs extracellular vesicle docking at target neurons.

Autor: Stajano D; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH University Medical Center Hamburg-Eppendorf Hamburg Germany., Lombino FL; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH University Medical Center Hamburg-Eppendorf Hamburg Germany., Schweizer M; Core Facility Morphology, Center for Molecular Neurobiology, ZMNH University Medical Center Hamburg-Eppendorf Hamburg Germany., Glatzel M; Institute of Neuropathology University Medical Center Hamburg-Eppendorf Hamburg Germany., Saftig P; Biochemical Institute Christian-Albrechts-University Kiel Kiel Germany., Gromova KV; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH University Medical Center Hamburg-Eppendorf Hamburg Germany., Kneussel M; Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH University Medical Center Hamburg-Eppendorf Hamburg Germany.

Publikováno v: Journal of extracellular biology [J Extracell Biol] 2023 Sep 11; Vol. 2 (9), pp. e113. Date of Electronic Publication: 2023 Sep 11 (Print Publication: 2023).