Comparative Analysis of T Cell Spatial Proteomics and the Influence of HIV Expression

Autor: John C. Guatelli, Charlotte A. Stoneham, Mary K. Lewinski, Aaron L. Oom, Alicia L. Richards, Km Shams-Ud-Doha, Jacob M. Wozniak, David Gonzalez, Nevan J. Krogan

As systems biology approaches to virology have become more tractable, highly studied viruses such as HIV can now be analyzed in new, unbiased ways, including spatial proteomics. We employed here a differential centrifugation protocol to fractionate J

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b43eae77d0170f30a3655183978aaf16
https://doi.org/10.1101/2021.03.17.435902

In situ detection of protein interactions for recombinant therapeutic enzymes

Autor: Km Shams-Ud-Doha, Sara Petersen Bjørn, Mojtaba Samoudi, Alexandre Rosa Campos, Caressa M Robinson, Stefan Kol, Chih-Chung Kuo, Nathan E. Lewis, Linus Weiss, Bjørn G. Voldborg, Song-Min Schinn

Publikováno v: Biotechnology and bioengineering, vol 118, iss 2
Biotechnol Bioeng
Samoudi, M, Chung Kuo, C, Robinson, C M, Shams-Ud-Doha, K, Schinn, S-M, Kol, S, Weiss, L, Petersen Bjørn, S, Voldborg, B G, Rosa Campos, A & Lewis, N E 2021, ' In situ detection of protein interactions for recombinant therapeutic enzymes ', Biotechnology and Bioengineering, vol. 118, no. 2, pp. 890-904 . https://doi.org/10.1002/bit.27621

Despite their therapeutic potential, many protein drugs remain inaccessible to patients since they are difficult to secrete. Each recombinant protein has unique physicochemical properties and requires different machinery for proper folding, assembly,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d8f0540ae6dd0a70f166b8a5419f2d2
https://doi.org/10.1101/2020.05.06.081885

Screening Oligosaccharide Libraries against Lectins Using the Proxy Protein Electrospray Ionization Mass Spectrometry Assay

Autor: Ling Han, Km Shams-Ud-Doha, Elena N. Kitova, John S. Klassen

Publikováno v: Analytical Chemistry. 88:8224-8231

An electrospray ionization mass spectrometry (ESI-MS) assay for screening carbohydrate libraries against lectins is described. The assay is based on the proxy protein ESI-MS method, which combines direct ESI-MS protein-ligand binding measurements and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6001cf82dc333260880e24661e1e6238
https://doi.org/10.1021/acs.analchem.6b02044

Antinociceptive, anti-inflammatory, antimicrobial and central nervous system depressant activities of ethanolic extract of leaves and roots of Gomphostemma parviflorum var. parviflorum wall

Autor: Sitesh C. Bachar, Nazmul Qais, Zobaer Al Mahmud, KM Shams-Ud-Doha

Publikováno v: Pharmacognosy Research

Background: Gomphostemma parviflorum (Lamiaceae) is a medicinal plant of Bangladesh which has been used traditionally in the treatment of painful and inflammatory conditions such as asthma, headache, fever, etc. Objective: To investigate the antinoci

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdb8a1bc6d2e7123fc1c6e89a5422d9a
http://europepmc.org/articles/PMC3807986

Quantifying protein-carbohydrate interactions using liquid sample desorption electrospray ionization mass spectrometry

Autor: John S. Klassen, Rambod Daneshfar, Km Shams-Ud-Doha, Elena N. Kitova, Yuyu Yao

Publikováno v: Journal of the American Society for Mass Spectrometry. 26(1)

The application of liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) for quantifying protein–carbohydrate interactions in vitro is described. Association constants for the interactions between lysozyme and �

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13463f7d2bc5c9a7e68fa1d835708100
https://pubmed.ncbi.nlm.nih.gov/25315460