Protocol development to further differentiate and transition stem cell-derived pancreatic progenitors from a monolayer into endocrine cells in suspension culture.

Autor: Braam MJS; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada., Zhao J; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada., Liang S; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada., Ida S; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada., Kloostra NK; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada., Iworima DG; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada.; School of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada., Tang M; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada., Baker RK; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada., Quiskamp N; STEMCELL Technologies, Vancouver, BC, Canada., Piret JM; Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada.; Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, BC, Canada.; School of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada., Kieffer TJ; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada. tim.kieffer@ubc.ca.; School of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada. tim.kieffer@ubc.ca.; Department of Surgery, University of British Columbia, Vancouver, BC, Canada. tim.kieffer@ubc.ca.

Publikováno v: Scientific reports [Sci Rep] 2023 Jun 01; Vol. 13 (1), pp. 8877. Date of Electronic Publication: 2023 Jun 01.