Primary recovery of a genetically engineeredTrichoderma reeseiendoglucanase I (Cel 7B) fusion protein in cloud point extraction systems

Autor: Michael Bailey, Anna Collén, Merja Penttilä, Josefine Persson, Folke Tjerneld, Klaus Selber, Maria-Regina Kula, Henrik Stålbrand, Tiina Nakari-Setlä, Richard Fagerström, Teppo Hyytiä

Publikováno v: Biotechnology and Bioengineering. 78:385-394

Here we present data to demonstrate how partitioning of a hydrophilic enzyme can be directed to the hydrophobic detergent-enriched phase of an aqueous two-phase system by addition of short stretches of amino acid residues to the protein molecule. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4edc9f0376be82a66a3574a69a8e7986
https://doi.org/10.1002/bit.10232

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Autor: Jörg Thömmes, Thomas Sottmann, Holger Gieren, Klaus Selber, Stefan Müller, Maria-Regina Kula, Reinhard Strey

Publikováno v: Bioseparation. 10:243-254

The physical behavior of the binary phase systems of the non-ionic polyoxyethylene detergent Agrimul NRE 1205 and water was investigated. This technical detergent can be used for the large-scale recovery of biomolecules in detergent based aqueous two

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1bde99368805ca70d4cf0ed873c3da9f
https://doi.org/10.1023/a:1016331228174

[Untitled]

Autor: M.-R. Kula, A. Rodenbrock, Klaus Selber, Maarten R. Egmond

Publikováno v: Bioseparation. 9:269-276

Detergent-based aqueous two-phase systems have the advantage to require only one auxiliary chemical to induce phase separation above the cloud point. In a systematic study the efficiency of tryptophan-rich peptide tags was investigated to enhance the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::645e3d431c92706738ffa6f38950fff2
https://doi.org/10.1023/a:1011190713820

Large-scale separation and production of engineered proteins, designed for facilitated recovery in detergent-based aqueous two-phase extraction systems

Autor: Anna Collén, Merja Penttilä, Tiina Nakari-Setälä, Richard Fagerström, Joop van der Laan, M.-R. Kula, Folke Tjerneld, Michael Bailey, John Kan, Klaus Selber, Teppo Hyytiä

Publikováno v: Selber, K, Tjerneld, F, Collén, A, Hyytiä, T, Nakari-Setälä, T, Bailey, M, Fagerström, R, Kan, J, Van Der Laan, J, Penttilä, M & Kula, M R 2004, ' Large-scale separation and production of engineered proteins, designed for facilitated recovery in detergent-based aqueous two-phase extraction systems ', Process Biochemistry, vol. 39, no. 7, pp. 889-896 . https://doi.org/10.1016/S0032-9592(03)00198-5

The feasibility and scalability of extraction in detergent-based aqueous two-phase systems for the separation of proteins from culture broth is demonstrated. At the same time the large-scale production of a fusion protein and the influence of cultiva

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd2c4d741bb39609bb8247ea5150bbc1
https://cris.vtt.fi/en/publications/1b950585-61f9-44cd-9b5d-c7847ea65783

Primary recovery of a genetically engineered Trichoderma reesei endoglucanase I (Cel 7B) fusion protein in cloud point extraction systems

Autor: Anna, Collén, Klaus, Selber, Teppo, Hyytiä, Josefine, Persson, Tiina, Nakari-Setlä, Michael, Bailey, Richard, Fagerström, Maria-Regina, Kula, Merja, Penttilä, Henrik, Stålbrand, Folke, Tjerneld

Publikováno v: Biotechnology and bioengineering. 78(4)

Here we present data to demonstrate how partitioning of a hydrophilic enzyme can be directed to the hydrophobic detergent-enriched phase of an aqueous two-phase system by addition of short stretches of amino acid residues to the protein molecule. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4918ac3ba5ff5853d3632bb49de6119f
https://pubmed.ncbi.nlm.nih.gov/11948445