Výsledky vyhledávání - "Klaus Peter Koller"

Akademický článek

An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

Autor: Piotr M. Skowron, Daria Krefft, Robert Brodzik, Paulina Kasperkiewicz, Marcin Drag, Klaus-Peter Koller

Publikováno v: Microbial Cell Factories, Vol 19, Iss 1, Pp 1-15 (2020)

Abstract Background A neutral, heat-sensitive serine protease (NHSSP) originating from the feather-degrading fungus Onygena corvina (O. corvina) was described and defined as an alkaline serine protease of the subtilisin type S8 family, exhibiting an

Externí odkaz: https://doaj.org/article/16a9a87e011e438f8f2cf6fde5a19e32

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Autor: Klaus-Peter Koller, Piotr M. Skowron, Marcin Drag, Daria Krefft, Robert Brodzik, Paulina Kasperkiewicz

Publikováno v: Microbial Cell Factories
Microbial Cell Factories, Vol 19, Iss 1, Pp 1-15 (2020)

Background A neutral, heat-sensitive serine protease (NHSSP) originating from the feather-degrading fungus Onygena corvina (O. corvina) was described and defined as an alkaline serine protease of the subtilisin type S8 family, exhibiting an enzymatic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::132f09fa701938ff669019fcdff2d281
https://doi.org/10.1186/s12934-020-01392-3

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Additional file 3 of An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

Autor: Skowron, Piotr M., Krefft, Daria, Brodzik, Robert, Kasperkiewicz, Paulina, Drag, Marcin, Klaus-Peter Koller

Additional file 3. Synthetic DNA fragment sequence, translation and features, comprising optimized full length SP-PRO-NHSSP-His6 gene. Secretion peptide native—marked in green, propeptide—marked in yellow, His6-tag—marked in red.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5225334c22fca574029b9cbe1c04c565

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Additional file 9 of An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

Autor: Skowron, Piotr M., Krefft, Daria, Brodzik, Robert, Kasperkiewicz, Paulina, Drag, Marcin, Klaus-Peter Koller

Additional file 9. Synthetic DNA fragment sequence, translation and features, comprising optimized SP-PRO-NHSSP gene with deleted C-terminal 4 aa and His6-tag. Secretion peptide native—marked in green, propeptide—marked in yellow.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42c1d71e06105b78f4f40ead3362ad6c

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Effects of Subinhibitory Concentrations of Antibiotics on Alpha-Toxin ( hla ) Gene Expression of Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus Isolates

Autor: Klaus-Peter Koller, Thomas A. Wichelhaus, Wilma Ziebuhr, Knut Ohlsen, Wolfgang Hell, Jörg Hacker

Publikováno v: Antimicrobial Agents and Chemotherapy. 42:2817-2823

Concentrations of antibiotics below the MIC are able to modulate the expression of virulence-associated genes. In this study, the influence of subinhibitory doses of 31 antibiotics on the expression of the gene encoding the staphylococcal alpha-toxin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53a3abee3525ee34f4816a7f0568000a
https://doi.org/10.1128/aac.42.11.2817

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Folding of the disulfide-bonded β-sheet protein tendamistat: rapid two-state folding without hydrophobic collapse 1 1Edited by A. R. Fersht

Autor: Klaus-Peter Koller, Nancy Schönbrunner, Thomas Kiefhaber

Publikováno v: Journal of Molecular Biology. 268:526-538

We investigated the reversible folding and unfolding reactions of the small 74 amino acid residue protein tendamistat. The secondary structure of tendamistat contains only β-sheets and loop regions and the protein contains two disulfide bonds. Fluor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2d04e796a9aa4632f874676d02048f4d
https://doi.org/10.1006/jmbi.1997.0960

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