Výsledky vyhledávání - "Klaus H. Seifart"

Assembly and isolation of intermediate steps of transcription complexes formed on the human 5S rRNA gene

Autor: Wolfgang Meissner, Klaus H. Seifart, Stephan Weser, Jana Riemann

Publikováno v: Nucleic Acids Research. 31:2408-2416

By employing purified transcription factors and RNA polymerase III (pol III), we generated active pol III transcription complexes on the human 5S rRNA gene. These large complexes were separated by size exclusion chromatography from non- incorporated

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b66caedfb641cafe95897ccef133c319
https://doi.org/10.1093/nar/gkg345

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Development of an inducible pol III transcription system essentially requiring a mutated form of the TATA-binding protein

Autor: Heike Rothfels, Wolfgang Meissner, Beatrix Schäfer, Klaus H. Seifart

Publikováno v: Nucleic Acids Research. 29:1672-1682

We attempted to devise a transcription system in which a particular DNA sequence of interest could be inducibly expressed under the control of a modified polymerase III (pol III) promoter. Its activation requires a mutated transcription factor not co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c61bf02473473a62e1b457cd74b8395
https://doi.org/10.1093/nar/29.8.1672

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hTFIIIB-β stably binds to pol II promoters and recruits RNA polymerase III in a hTFIIIC1 dependent way 1 1Edited by M. Yaniv

Autor: Ingo Kober, Klaus H. Seifart, Martin Teichmann

Publikováno v: Journal of Molecular Biology. 284:7-20

It has been shown that under specific conditions, transcription of protein coding genes can be efficiently initiated by RNA polymerase (pol) III in vitro. We examined the formation and composition of such pol III transcription complexes on the duck h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::81f2746ec822175362de57467fce01eb
https://doi.org/10.1006/jmbi.1998.2165

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The activity binding to the termination region of several pol III genes represents a separate entity and is distinct from a novel component enhancing U6 snRNA transcription

Autor: Susanne Oettel, Ingo Kober, Klaus H. Seifart

Publikováno v: Nucleic Acids Research. 26:4324-4331

Human TFIIIC1, a basal transcription factor essentially required for expression of all pol III genes, exerts its function without primarily binding to DNA. We report here the purification of a termination site binding activity (TBA) which was initial

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3b317f3ab0136ea9dbceddbbe1a4a96
https://doi.org/10.1093/nar/26.19.4324

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Human transcription factors IIIC2, IIIC1 and a novel component IIIC0 fulfil different aspects of DNA binding to various pol III genes

Autor: Ingo Kober, Sebastian Iben, Frauke Härtel, Klaus H. Seifart, Susanne Oettel

Publikováno v: Nucleic Acids Research. 25:2440-2447

Human transcription factor IIIC2 interacts with the TFIIIA-5S DNA complex and forms a ternary TFIIIA/IIIC2-5S DNA complex. Formation of this complex does not preclude simultaneous binding of TFIIIC2to the B-box sequence of a second template. This sug

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::756c739f698eb3606c474c24ef081f85
https://doi.org/10.1093/nar/25.12.2440

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The human S3a ribosomal protein: sequence, location and cell-free transcription of the functional gene

Autor: Dagmar Nolte, Martha Kalff-Suske, Gerhild Taimor, Klaus H. Seifart

Publikováno v: Gene. 169:179-185

The intron-containing gene encoding human ribosomal protein S3a (hRPS3a) was isolated by utilizing a PCR-based strategy to detect a gene-specific intron which was subsequently used as a probe for cloning of the entire gene. The hRPS3a gene is compose

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::478555843e168502e02faab7ff1159ef
https://doi.org/10.1016/0378-1119(95)00708-3

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Human TFIIIA alone is sufficient to prevent nucleosomal repression of a homologous 5S gene

Autor: Klaus H. Seifart, Walter Stunkel, Ingo Kober, Gerhild Taimor, Manfred Kauer

Publikováno v: Nucleic Acids Research. 23:109-116

Plasmid DNA harbouring the human 5S rRNA gene was assembled into nucleosomes using either Xenopus S150 extracts or purified core histones in the presence of pectin. In both cases reconstitution of nucleosomes led to a complete repression of transcrip

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9263e7e0f187cb949869154403930d7e
https://doi.org/10.1093/nar/23.1.109

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TFIIA is required for in vitro transcription of mammalian U6 genes by RNA polymerase III

Autor: R Waldschmidt, Klaus H. Seifart

Publikováno v: Journal of Biological Chemistry. 267:16359-16364

Transcription factor TFIIA, defined by its role in transcription by RNA polymerase II, is also involved in RNA polymerase III transcription of mammalian U6 small nuclear RNA genes. This finding was substantiated by experimental evidence including (i)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::65d729b89591f62ca38ff5f4f2e5e9e3
https://doi.org/10.1016/s0021-9258(18)42010-8

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Transcription factor USF from duck erythrocytes transactivates expression of the histone H5 genein vitroby interacting with an intragenic sequence

Autor: Helmut Gerhold, Klaus H. Seifart, Frank Düring

Publikováno v: Nucleic Acids Research. 18:1225-1231

The duck histone H5 gene contains a 12 base pair (bp) sequence motif within the coding region, which shows homology in 10 out of 12 bp with the consensus sequence of the USF binding site in the Ad2ML-promoter. The functional equivalent of transcripti

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https://doi.org/10.1093/nar/18.5.1225

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