Ligand-induced conformational changes in the β1-adrenergic receptor revealed by hydrogen-deuterium exchange mass spectrometry

Autor: Joanna Toporowska, Parth Kapoor, Maria Musgaard, Karolina Gherbi, Kathy Sengmany, Feng Qu, Mark Soave, Hsin-Yung Yen, Kjetil Hansen, Ali Jazayeri, Jonathan T. S. Hopper, Argyris Politis

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)

Abstract G Protein Coupled Receptors (GPCRs) constitute the largest family of signalling proteins responsible for translating extracellular stimuli into intracellular functions. They play crucial roles in numerous physiological processes and are majo

Externí odkaz: https://doaj.org/article/888702c45a2847fb87032245b452250b

Structural basis for isoform-specific kinesin-1 recognition of Y-acidic cargo adaptors

Autor: Stefano Pernigo, Magda S Chegkazi, Yan Y Yip, Conor Treacy, Giulia Glorani, Kjetil Hansen, Argyris Politis, Soi Bui, Mark P Dodding, Roberto A Steiner

Publikováno v: eLife, Vol 7 (2018)

The light chains (KLCs) of the heterotetrameric microtubule motor kinesin-1, that bind to cargo adaptor proteins and regulate its activity, have a capacity to recognize short peptides via their tetratricopeptide repeat domains (KLCTPR). Here, using X

Externí odkaz: https://doaj.org/article/a58fc294a3834a8bbed2b1ec3afd638c

Cold Denaturation of Proteins in the Absence of Solvent: Implications for Protein Storage**

Autor: Emma L. Norgate, Rosie Upton, Kjetil Hansen, Bruno Bellina, C. Brookes, Argyris Politis, Perdita E. Barran

Publikováno v: Norgate, E L, Upton, R, Hansen, K, Bellina, B, Brookes, C, Politis, A & Barran, P E 2022, ' Cold Denaturation of Proteins in the Absence of Solvent: Implications for Protein Storage ', Angewandte Chemie International Edition . https://doi.org/10.1002/anie.202115047

The effect of temperature on the stability of proteins is well explored above 298 K, but harder to track experimentally below 273 K. Variable-temperature ion mobility mass spectrometry (VT IM-MS) allows us to measure the structure of molecules at sub

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cc75dc46cf1259e158467a523e3f09f
https://doi.org/10.1002/ange.202115047

Improving Peptide Fragmentation for Hydrogen-Deuterium Exchange Mass Spectrometry Using a Time-Dependent Collision Energy Calculator

Autor: Kjetil Hansen, Argyris Politis

Publikováno v: Journal of the American Society for Mass Spectrometry. 31(4)

Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is becoming a popular technique for interrogating biological systems. In recent years, advancements have been made to increase peptide coverage for proteins that resist digestion such as antibodi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f061b774d23ebeae96ac07bd124d1a6b
https://pubmed.ncbi.nlm.nih.gov/32141743

Glu-C, an alternative digestive enzyme for the quantitative LC-MS/MS analysis of an IgG-based antibody biotherapeutic

Autor: Emilie Escoffier, Amandine Berthet, Joris Venet, Justine Collet-Brose, Robert Nelson, Michael Wright, Robert Wheller, Sophie Hepburn, Richard G. Kay, Kjetil Hansen, Szabolcs Szarka

Publikováno v: Bioanalysis. 10(13)

Aim: LC–MS/MS bottom-up quantitation of proteins has become increasingly popular with trypsin as the most commonly used protease. However, trypsin does not always yield suitable surrogate peptides. An alternative enzyme, Glu-C, was used to generate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad6bc830b786877dc1d721a34b9ba09f
https://pubmed.ncbi.nlm.nih.gov/29972309