Výsledky vyhledávání - "Kjell-Ove Eriksson"

Zirconia particles

Autor: Gani, Mary Susan Jean, Hans-Jurgen Wirth, Aguilar, Marie Isabel, Hearn, Milton Thomas William, Vanselow, Donald George, Cheang, Philip Hong Ning, Kjell-Ove Eriksson

Porous zirconia or zirconium-containing particles, methods of making such particles and methods of using such particles including modifications to the surface of the particles are described. The method comprises heating zirconia particles to provide

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::89fd270d21dc1e751eca91751a510e1f

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A CASE STUDY: OLIGONUCLEOTIDE PURIFICATION FROM GRAM TO HUNDRED GRAM SCALE

Autor: Douglas L. Cole, Peter B. Moore, Yogesh S. Sanghvi, Kjell-Ove Eriksson, Ranjit R. Deshmukh

Publikováno v: Nucleosides, Nucleotides and Nucleic Acids. 20:567-576

This article describes the purification and scale-up of ISIS 2302, a 20-mer phosphorothioate oligonucleotide by anion-exchange (AX) chromatography. The key operating parameters were optimized at gram scale and further scaled up to hundred gram. SOURC

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0da1689f41a003ae46d106093baf6c93
https://doi.org/10.1081/ncn-100002333

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Hydrophobic Interaction Chromatography

Autor: Makonnen Belew, Kjell-Ove Eriksson

Publikováno v: Protein Purification

Hydrophobic interaction chromatography (HIC) is a widely used chromatographic technique that finds application at different stages of downstream processing. It was first described by Shepard and Tiselius in 1949 using the term “salting out chromato

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ee433b6a64b54ecb3c2b74f9eb6524f2
https://doi.org/10.1002/9780470939932.ch6

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General methods to render macroporous stationary phases nonporous and deformable, exemplified with agarose and silica beads and their use in high-performance ion-exchange and hydrophobic-interaction chromatography of proteins

Autor: Stellan Hjertén, Kjell-Ove Eriksson, Jia-Li Liao, Jamil Mohammad

Publikováno v: Chromatographia. 31:85-94

New general methods for the preparation of nonporous beads from macroporous beads are described. One method is based on filling the inner volume of the beads with a solution of a monomer which binds to the matrix at the same time as it is allowed to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9a33327f705c1715aa6ed9528dd5d465
https://doi.org/10.1007/bf02290503

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Real-time kinetic analysis of limited proteolysis by ion-exchange chromatography using compressed, non-porous agarose beads

Autor: Gunnar Johansson, Kjell-Ove Eriksson, Jamil Mohammad

Publikováno v: Journal of biochemical and biophysical methods. 26(1)

In biotechnology there is a great need for methods that can be used for kinetic studies of ongoing processes, such as limited proteolysis. Ion-exchange chromatography on a nonporous, compressed agarose matrix was used in the study presented in this p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f650123b49deea13558b1890ffe55062
https://pubmed.ncbi.nlm.nih.gov/8482789

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Preparative capillary electrophoresis based on adsorption of the solutes (proteins) onto a moving blotting membrane as they migrate out of the capillary

Autor: Stellan Hjertén, Anders Palm, Kjell-Ove Eriksson

Publikováno v: Analytical biochemistry. 201(2)

A micropreparative capillary electrophoresis apparatus equipped with a new type of fraction collection device is described: solutes, such as proteins, are adsorbed onto a moving blotting membrane (for instance a polyvinylidene difluoride membrane) as

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b129eac27146b0172e1779d95eef5a7a
https://pubmed.ncbi.nlm.nih.gov/1632508

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Precolumn concentration of protein samples by displacement electrophoresis (isotachophoresis) followed by high-performance molecular sieve chromatography on a packed agarose column

Autor: Kjell-Ove Eriksson, Lars-Göran Öfverstedt

Publikováno v: Analytical Biochemistry. 137:318-323

The use of displacement electrophoresis for the concentration of dilute protein solutions and the construction of a column suitable for this purpose are described. The concentrated protein zone can be pumped directly from the electrophoresis column i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23226a94e085500f8ae65206f70281f1
https://doi.org/10.1016/0003-2697(84)90092-7

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Surface hydrophobicity and electrophoretic mobilities of staphylococcal exotoxins with special reference to toxic shock syndrome toxin-1

Autor: Torkel Wadström, Ferenc Kilár, Stellan Hjertén, A. Satyanarayan Naidu, Kjell-Ove Eriksson

Publikováno v: APMIS. 97:1081-1087

The surface hydrophobicities of eleven staphylococcal toxins were estimated and compared with those of standard proteins on an octyl agarose column by high-performance hydrophobic-interaction chromatography (HP-HIC). Staphylococcal enterotoxins (SE)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c71aafe77ad4773082ccb6b06332a58d
https://doi.org/10.1111/j.1699-0463.1989.tb00521.x

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