Zobrazeno 1 - 10
of 104
pro vyhledávání: '"Kiyoshi Shiga"'
Autor:
Atsushi Yamamoto, Takayuki Koyama, D. Nozaki, Kiyoshi Shiga, Tatsuya Iwata, Yasuzo Nishina, Satoru Tokutomi, Masashi Unno, Hideki Kandori
Publikováno v:
Biochemistry. 56(24)
The light oxygen voltage (LOV) domain is a flavin-binding blue-light receptor domain, originally found in a plant photoreceptor phototropin (phot). Recently, LOV domains have been used in optogenetics as the photosensory domain of fusion proteins. Th
Publikováno v:
Journal of Biochemistry. 154:61-66
Electron-transferring flavoprotein (ETF) from Megasphaera elsdenii contains two FAD molecules, FAD-1 and FAD-2. FAD-2 shows an unusual absorption spectrum with a 400-nm peak. In contrast, ETFs from other sources such as pig contain one FAD and one AM
Publikováno v:
Journal of Biochemistry. 153:565-572
Electron-transferring flavoprotein (ETF) from the anaerobic bacterium Megasphaera elsdenii is a heterodimer containing two FAD cofactors. Isolated ETF contains only one FAD molecule, FAD-1, because the other, FAD-2, is lost during purification. FAD-2
Autor:
Seiichi Era, Masaru Sogami, Kazuo Kuwata, Shunji Nagaoka, Kiyoshi Shiga, Kazuyuki Akasaka, Hiroshi Watari, Kimihiro Kida, Hiroshi Inouye
Publikováno v:
International Journal of Peptide and Protein Research. 28:130-140
Bovine plasma albumin Fr. V (BPA) has been known to contain small amounts of proteolytic enzyme. Wilson & Foster (1971) found a very limited and specific cleavage of BPA catalyzed by the enzyme with BPA in the F-form near pH 3.8, resulting in the for
Publikováno v:
Journal of Photochemistry and Photobiology B: Biology. 90:134-140
Electron-transferring flavoprotein (Holo-ETF) from Megasphaera elsdenii contains two FAD's, one of which easily dissociates to form Iso-ETF (contains one FAD). Time-resolved fluorescence of FAD in Iso-ETF, and Holo-ETF were measured at 5 degrees C an
Autor:
Noboru Mataga, Haik Chosrowjan, Kyosuke Sato, Yasuzo Nishina, Fumio Tanaka, Seiji Taniguchi, Kiyoshi Shiga
Publikováno v:
The Journal of Physical Chemistry B. 111:5694-5699
Ultrafast fluorescence quenching of flavin in flavodoxin from Megasphaera elsdenii was investigated by means of a fluorescence up-conversion method. Fluorescence lifetimes of flavodoxin from M. elsdenii were estimated to be tau(1) approximately 165 f
Autor:
D. Nozaki, Hideki Kandori, Kyosuke Sato, Yoshiaki Sato, Satoru Tokutomi, Yasuzo Nishina, Tatsuya Iwata, Kiyoshi Shiga
Publikováno v:
Biochemistry. 45:15384-15391
Phototropin, a blue-light photoreceptor in plants, has two FMN-binding domains named LOV1 and LOV2. We previously observed temperature-dependent FTIR spectral changes in the C=O stretching region (amide-I vibrational region of the peptide backbone) f
Autor:
Shinji Ito, Shoichi Higashi, Mineo Iseki, Tetsuo Takahashi, Yasuzo Nishina, Akio Murakami, Masakatsu Watanabe, Kyosuke Sato, Kiyoshi Shiga
Publikováno v:
Photochemical and Photobiological Sciences = Photochemical and Photobiological Sciences. 4(9):762-769
Photoactivated adenylyl cyclase (PAC) is a recently discovered blue-light photoreceptor that mediates photomovement in Euglena gracilis(Iseki et al., Nature, 2002, 415, 1047--1051). PAC appears to be a heterotetramer composed of two FAD-binding subun
Publikováno v:
Journal of Photochemistry and Photobiology B: Biology. 70:67-73
The dynamic natures of two hydrogen-bonding model systems, riboflavin tetrabutylate (RFTB)-trichloroacetic acid (TCA) and RFTB-phenol in benzene, and of electron-transferring flavoprotein (ETF) from pig kidney upon excitation of flavins was investiga
Autor:
Michizo Sugai, Mineo Iseki, Kazuichi Yoshida, Terumitsu Hori, Tetsuo Takahashi, Kiyoshi Shiga, Akio Murakami, Masakatsu Watanabe, Kaoru Ohno, Shigeru Matsunaga
Publikováno v:
Nature. 415:1047-1051
Blue light regulates processes such as the development of plants and fungi and the behaviour of microbes. Two types of blue-light receptor flavoprotein have been identified: cryptochromes, which have partial similarity to photolyases, and phototropin