Zobrazeno 1 - 10
of 214
pro vyhledávání: '"Kiyoshi Asai"'
Autor:
Yoichiro Ito, Misa Ishigami, Goro Terai, Yasuyuki Nakamura, Noriko Hashiba, Teruyuki Nishi, Hikaru Nakazawa, Tomohisa Hasunuma, Kiyoshi Asai, Mitsuo Umetsu, Jun Ishii, Akihiko Kondo
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-12 (2022)
A high-throughput workflow involving a streamlined genome-wide screen improves protein production in the non-conventional yeast, Komagataella phaffii, following adaptive laboratory evolution.
Externí odkaz:
https://doaj.org/article/f8d6b4a76d204cf09f7a566450ea6f5c
Publikováno v:
BMC Bioinformatics, Vol 21, Iss 1, Pp 1-19 (2020)
Abstract Background Analysis of secondary structures is essential for understanding the functions of RNAs. Because RNA molecules thermally fluctuate, it is necessary to analyze the probability distributions of their secondary structures. Existing met
Externí odkaz:
https://doaj.org/article/73f1f209cd4b4e1eb81137275f13e437
Autor:
Akito Taneda, Kiyoshi Asai
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 18, Iss , Pp 1811-1818 (2020)
Codon optimization in protein-coding sequences (CDSs) is a widely used technique to promote the heterologous expression of target genes. In codon optimization, a combinatorial space of nucleotide sequences that code a given amino acid sequence and ta
Externí odkaz:
https://doaj.org/article/d830716adb054c80969c17454e517f30
Publikováno v:
BMC Bioinformatics, Vol 19, Iss S1, Pp 85-95 (2018)
Abstract Background It is known that functional RNAs often switch their functions by forming different secondary structures. Popular tools for RNA secondary structures prediction, however, predict the single ‘best’ structures, and do not produce
Externí odkaz:
https://doaj.org/article/931a84668fd64b70be84d3a263748634
Publikováno v:
International Journal of Molecular Sciences, Vol 14, Iss 3, Pp 6144-6156 (2013)
Due to the recent discovery of non-coding RNAs (ncRNAs), multiple sequence alignment (MSA) of those long RNA sequences is becoming increasingly important for classifying and determining the functional motifs in RNAs. However, not only primary (nucleo
Externí odkaz:
https://doaj.org/article/6240ddd9e3aa41039caa8d9bfcf18018
Autor:
Tsutomu Ikegami, Toyohiro Inatsugi, Isao Kojima, Myco Umemura, Hiroko Hagiwara, Masayuki Machida, Kiyoshi Asai
Publikováno v:
PLoS ONE, Vol 10, Iss 4, p e0126289 (2015)
A hybrid de novo assembly pipeline was constructed to utilize both MiSeq and SOLiD short read data in combination in the assembly. The short read data were converted to a standard format of the pipeline, and were supplied to the pipeline components s
Externí odkaz:
https://doaj.org/article/35382af45e434493a869f86f951de2b4
Publikováno v:
PLoS ONE, Vol 8, Iss 1, p e54210 (2013)
Human gene catalogs are fundamental to the study of human biology and medicine. But they are all based on open reading frames (ORFs) in a reference genome sequence (with allowance for introns). Individual genomes, however, are polymorphic: their sequ
Externí odkaz:
https://doaj.org/article/4b1f2a6ea84748a9a08c9946ab04f715
Autor:
Myco Umemura, Hideaki Koike, Nozomi Nagano, Tomoko Ishii, Jin Kawano, Noriko Yamane, Ikuko Kozone, Katsuhisa Horimoto, Kazuo Shin-ya, Kiyoshi Asai, Jiujiang Yu, Joan W Bennett, Masayuki Machida
Publikováno v:
PLoS ONE, Vol 8, Iss 12, p e84028 (2013)
Many bioactive natural products are produced as "secondary metabolites" by plants, bacteria, and fungi. During the middle of the 20th century, several secondary metabolites from fungi revolutionized the pharmaceutical industry, for example, penicilli
Externí odkaz:
https://doaj.org/article/9e08be6951244d86b9b07cbead749857
Publikováno v:
PLoS ONE, Vol 7, Iss 9, p e44314 (2012)
MicroRNA (miRNA) precursor hairpins have a unique secondary structure, nucleotide length, and nucleotide content that are in most cases evolutionarily conserved. The aim of this study was to utilize position-specific features of miRNA hairpins to imp
Externí odkaz:
https://doaj.org/article/7575bc012ed846ff9746ddf00b6d309c
Publikováno v:
PLoS ONE, Vol 6, Iss 2, p e16450 (2011)
In a number of estimation problems in bioinformatics, accuracy measures of the target problem are usually given, and it is important to design estimators that are suitable to those accuracy measures. However, there is often a discrepancy between an e
Externí odkaz:
https://doaj.org/article/500da4ec405c4e1bb36ef8395e661464