Zobrazeno 1 - 10 of 336 pro vyhledávání: '"Kiyoshi, Yasukawa"'
1
Akademický článek
Activity-stability trade-off observed in variants at position 315 of the GH10 xylanase XynR
Autor: Tomoka Nakamura, Teisuke Takita, Kohei Kuwata, Kimihiko Mizutani, Bunzo Mikami, Satoshi Nakamura, Kiyoshi Yasukawa
Publikováno v: Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024)
Abstract XynR is a thermostable alkaline GH10 xylanase, for which we have previously examined the effects of saturation mutagenesis at position 315 on enzyme alkaliphily, and found that at pH 10, the activities of variants could be ordered as follows
Externí odkaz: https://doaj.org/article/3d4e5e5160a24dd5b0c1d6cd6d9a1d4f
Zobrazit plný text záznamu
2
Akademický článek
Adenosine triphosphate induces amorphous aggregation of amyloid β by increasing Aβ dynamics
Autor: Masahiro Kuramochi, Momoka Nakamura, Hiroto Takahashi, Tomoe Komoriya, Teisuke Takita, Ngan Thi Kim Pham, Kiyoshi Yasukawa, Kazuaki Yoshimune
Publikováno v: Scientific Reports, Vol 14, Iss 1, Pp 1-7 (2024)
Abstract Amyloid β (Aβ) aggregates into two distinct fibril and amorphous forms in the brains of patients with Alzheimer’s disease. Adenosine triphosphate (ATP) is a biological hydrotrope that causes Aβ to form amorphous aggregates and inhibit f
Externí odkaz: https://doaj.org/article/b2dc2d147bef4a2e9a2260e6adbfe053
Zobrazit plný text záznamu
3
Akademický článek
Development of robust isothermal RNA amplification assay for lab-free testing of RNA viruses
Autor: Radhika Biyani, Kirti Sharma, Kenji Kojima, Madhu Biyani, Vishnu Sharma, Tarun Kumawat, Kevin Maafu Juma, Itaru Yanagihara, Shinsuke Fujiwara, Eiichi Kodama, Yuzuru Takamura, Masahiro Takagi, Kiyoshi Yasukawa, Manish Biyani
Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Abstract Simple tests of infectiousness that return results in minutes and directly from samples even with low viral loads could be a potential game-changer in the fight against COVID-19. Here, we describe an improved isothermal nucleic acid amplific
Externí odkaz: https://doaj.org/article/bcb2e1192fba4d1c9c295200b180c00e
Zobrazit plný text záznamu
5
Akademický článek
Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection.
Autor: Yukiko Nakura, Heng Ning Wu, Yuya Okamoto, Muneyuki Takeuchi, Koichiro Suzuki, Yoshitaka Tamura, Yuichiro Oba, Fumiko Nishiumi, Nobuaki Hatori, Shinsuke Fujiwara, Kiyoshi Yasukawa, Shinobu Ida, Itaru Yanagihara
Publikováno v: PLoS ONE, Vol 16, Iss 6, p e0252789 (2021)
The general methods to detect the RNA of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) in clinical diagnostic testing involve reverse transcriptases and thermostable DNA polymerases. In this study, we compared the detection of SARS-CoV
Externí odkaz: https://doaj.org/article/270fb753b23e44df8fcf89c395ac2a04
Zobrazit plný text záznamu
7
Akademický článek
Val143 of human ribonuclease H2 is not critical for, but plays a role in determining catalytic activity and substrate specificity.
Autor: Misato Baba, Kenji Kojima, Takuto Nishimura, Takuya Sugiura, Teisuke Takita, Ryo Uehara, Robert J Crouch, Kiyoshi Yasukawa
Publikováno v: PLoS ONE, Vol 15, Iss 2, p e0228774 (2020)
Ribonuclease H2 (RNase H2) exhibits both single ribonucleotide excision activity (activity A) and RNA strand degrading activity (activity B). Val143 of human RNase H2 is located at the active site and is conserved in eukaryotic RNase H2. In this stud
Externí odkaz: https://doaj.org/article/a468d7cf962e4865bd69d59ba1e26f0c
Zobrazit plný text záznamu
8
Akademický článek
Construction and characterization of ribonuclease H2 C subunit-knockout NIH3T3 cells.
Autor: Haruka Hara, Haruna Yano, Kaho Akazawa, Kana Kamoda, Mako Kandabashi, Misato Baba, Teisuke Takita, Kiyoshi Yasukawa
Publikováno v: Bioscience, Biotechnology & Biochemistry; Aug2023, Vol. 87 Issue 8, p865-876, 12p
Zobrazit plný text záznamu
10
Purification and characterization of a serine protease from the fruit of Ficus carica cultivar Masui Dauphine
Autor: Mizuki Tokonami, Akari Horiguchi, Naoki Ueshima, Teisuke Takita, Toru Takahashi, Kosaku Nishimura, Kiyoshi Yasukawa
Publikováno v: Bioscience, Biotechnology, and Biochemistry.
Ficus carica produces, in addition to the cysteine protease ficin, a serine protease (FSP). Here, we purified FSP to homogeneity from the fruit of F. carica cultivar Masui Dauphine. An 81-fold enrichment in specific activity of FSP with 2.1% recovery
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b1601862fdb696bd858bafb3721399d8
https://doi.org/10.1093/bbb/zbad028
Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání

Omezení vyhledávání
Plný text Recenzováno Digitální knihovna AV ČR
Zdroje