Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Kiyohiko Seki"'
Publikováno v:
Journal of Bioscience and Bioengineering. 127:425-429
An exo-chitosanase was purified from the culture filtrate of Gongronella butleri NBRC105989 to homogeneity by ammonium sulfate precipitation, followed by column chromatography using CM-Sephadex C-50 and Sephadex G-100. The enzyme comprised a monomeri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e014168263ac44670cd410c92373ff82
https://doi.org/10.1016/j.jbiosc.2018.09.009
https://doi.org/10.1016/j.jbiosc.2018.09.009
Autor:
Masaru Mitsutomi, Katsuaki Hirano, Akikazu Ando, Masamichi Watanabe, Akihiro Saito, Kiyohiko Seki
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 76:1932-1937
The hydrolytic specificities of chitosanases were determined using N¹,N⁴-diacetylchitohexaose [(GlcN)₂-GlcNAc-(GlcN)₂-GlcNAc]. The results for the hydrolytic specificities of chitosanases belonging to subclasses I, II, and III toward chitohexa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::93d41e4e337de992e7e347cad47261e2
https://doi.org/10.1271/bbb.120408
https://doi.org/10.1271/bbb.120408
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 76:1523-1528
Chitosanase II was purified from the culture filtrate of Aspergillus fumigatus ATCC13073. The purified enzyme had a molecular mass of 23.5 kDa. The N-terminal amino acid sequence of chitosanase II was identical to those of other Aspergillus chitosana
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::520e3f4e1c56e3d6a0a8e643104ecbb5
https://doi.org/10.1271/bbb.120248
https://doi.org/10.1271/bbb.120248
Autor:
Kosei Yasumoto, Tatsuo Suzuki, Masahiro Shoji, Kiichiro Teruya, Sanetaka Shirahata, Tadaaki Imai, Kozo Nakanishi, Hisashi Nakahashi, Masatoshi Kato, Takashi Yoshimatsu, Seiji Kawamoto, Kiyohiko Seki, Hiroki Murakami, Takeshi Hanagiri, Katsumi Mochizuki, Akira Nagashima, Kikuo Nomoto, Shuichi Hashizume, Yuko Setoguchi
Publikováno v:
Human Antibodies. 7:27-36
Human monoclonal antibody (hMAb) AE6F4 has been shown to be potentially useful for immunocytological detection of lung cancer cells in sputum. By recombinant DNA technology, IgM type hMAb AE6F4 was switched to lgG. The IgG mimic recombinant AE6F4 ant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bf22e654c9502a2a46fb55f5e6a4edf
https://doi.org/10.3233/hab-1996-7104
https://doi.org/10.3233/hab-1996-7104
Autor:
Sanetaka Shirahata, Tadaaki Imai, Tatsuo Suzuki, Kikuo Nomoto, Kiyohiko Seki, Katsumi Mochizuki, Akira Nagashima, Kosei Yasumoto, Shuichi Hashizume, Kiichiro Teruya, Yuichi Inoue, Hisashi Nakahashi, Seiji Kawamoto, Masatoshi Kato
Publikováno v:
Journal of Fermentation and Bioengineering. 81:466-469
The amplified ras oncogene was used to enhance the productivity of BHK-21 cells producing a lung cancer-reactive human recombinant AE6F4 monoclonal antibody. The recombinant antibody (18 mg) was produced by the ras-amplified BHK-21 cells in a one-mon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::195f88ebbdb38935ce692ecfbaa89795
https://doi.org/10.1016/0922-338x(96)85150-6
https://doi.org/10.1016/0922-338x(96)85150-6
Autor:
Kikuo Nomoto, Lourdes Bouzo Lopez, Tadaaki Imai, Mitsuhiro Takenoyama, Yoshinori Katakura, Masanori Kamei, Kiichiro Teruya, Masahiro Shoji, Hisashi Nakahashi, Kiyohiko Seki, Akira Nagashima, Hirofumi Tachibana, Sanetaka Shirahata, Kosei Yasumoto, Tatsuo Suzuki, Nobuhisa Arita, Shuichi Hashizume, Yuichi Inoue, Seiji Kawamoto, Yasumitsu Shiozawa, Hideya Ohashi
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 60:811-817
A ras oncogene-amplified recombinant BHK-21 cell line (ras-rBHK-IgG) has been established, and was shown to hyperproduce the recombinant IgG chimeric human monoclonal antibody (hMAb) AE6F4, which recognizes lung cancer cells. We found that the ras-rB
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccfb72e66cd99585fc415207e75e0aa9
https://doi.org/10.1271/bbb.60.811
https://doi.org/10.1271/bbb.60.811
Autor:
Katsuaki, Hirano, Masamichi, Watanabe, Kiyohiko, Seki, Akikazu, Ando, Akihiro, Saito, Masaru, Mitsutomi
Publikováno v:
Bioscience, biotechnology, and biochemistry. 76(10)
The hydrolytic specificities of chitosanases were determined using N¹,N⁴-diacetylchitohexaose [(GlcN)₂-GlcNAc-(GlcN)₂-GlcNAc]. The results for the hydrolytic specificities of chitosanases belonging to subclasses I, II, and III toward chitohexa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::ae26c813db1f034f81916c6737ff34c9
https://pubmed.ncbi.nlm.nih.gov/23047111
https://pubmed.ncbi.nlm.nih.gov/23047111
Autor:
Hideya Ohashi, Kiyohiko Seki, Hiroki Murakami, Sanetaka Shirahata, Kiichiro Teruya, Takahiro Yano, Hirofumi Tachibana
Publikováno v:
Analytical Biochemistry. 214:468-473
We have developed a rapid and sensitive screening method to detect the secretory product from transformed secretory cells. Polyclonal antibodies against the target secretory protein are first adsorbed onto a nitrocellulose membrane and are then calle
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::878e7adbe3640db62e293f16fb02d58c
https://doi.org/10.1006/abio.1993.1524
https://doi.org/10.1006/abio.1993.1524
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1182:257-263
The carbohydrate chains on the light chains of human monoclonal antibody HB4C5 reactive to human lung adenocarcinoma tissue have been characterized. The HB4C5 antibody consistst of two kinds of light chains (30 kDa and 32 kDa). Each chain has differe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e977860c8bfd16d3e1e73d88a986667
https://doi.org/10.1016/0925-4439(93)90067-b
https://doi.org/10.1016/0925-4439(93)90067-b
Autor:
Hiroki Murakami, Sanetaka Shirahata, Koji Yamada, Hirofumi Tachibana, Tetsuya Mori, Kiyohiko Seki
Publikováno v:
Agricultural and Biological Chemistry. 55:1689-1691
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab4df0b300de6074d6108ffe2821c941
https://doi.org/10.1271/bbb1961.55.1689
https://doi.org/10.1271/bbb1961.55.1689