Effectiveness of iodine for continuous decontamination of dental unit waterline

Autor: Chatchaya Petchphayaprai, Chutimon Chotipan, Pitchayapa Sa-ngiampak, Kittisak Thotsaporn, Ruchanee Salingcarnboriboon Ampornaramveth

Publikováno v: BDJ Open, Vol 9, Iss 1, Pp 1-5 (2023)

Abstract Objective Dental Unit Waterlines (DUWLs) are contaminated by various species of microorganisms. DUWLs should be disinfected appropriately to control microbial contamination. This study investigated the effectiveness of devices continuously r

Externí odkaz: https://doaj.org/article/4af81c9fc51a403aa4f425b7d16f1393

Enzymes in the p-hydroxyphenylacetate degradation pathway of Acinetobacter baumannii

Autor: Kittisak Thotsaporn, Ruchanok Tinikul, Pimchai Chaiyen, Pirom Chenprakhon, Somchart Maenpuen, Jittima Phonbuppha, Pratchaya Watthaisong

Publikováno v: Journal of Molecular Catalysis B: Enzymatic. 134:353-366

p-Hydroxyphenylacetate (HPA) can be derived from the biodegradation of lignin or from man-made compounds. The pathway involved for HPA degradation has been characterized for several species, but little is known on the degradation of HPA in Acinetobac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9fcad00a68ea220d700529e289d9da30
https://doi.org/10.1016/j.molcatb.2016.09.003

The C-terminal Domain of 4-Hydroxyphenylacetate 3-Hydroxylase from Acinetobacter baumannii Is an Autoinhibitory Domain

Autor: Worrapoj Oonanant, Jirundon Yuvaniyama, David P. Ballou, Jisnuson Svasti, Thanawat Phongsak, Pimchai Chaiyen, Kittisak Thotsaporn, Jeerus Sucharitakul

Publikováno v: Journal of Biological Chemistry. 287:26213-26222

p-Hydroxyphenylacetate (HPA) 3-hydroxylase from Acinetobacter baumannii consists of a reductase component (C(1)) and an oxygenase component (C(2)). C(1) catalyzes the reduction of FMN by NADH to provide FMNH(-) as a substrate for C(2). The rate of re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98df6f8bf2af83e333ace2bfc9625e66
https://doi.org/10.1074/jbc.m112.354472

Multiple pathways guide oxygen diffusion into flavoenzyme active sites

Autor: Pimchai Chaiyen, Marco W. Fraaije, Willem J. H. van Berkel, Pirom Chenprakhon, J. Andrew McCammon, Riccardo Baron, Andrea Mattevi, Conor T. Riley, Federico Forneris, Andrea Alfieri, Remko T. Winter, Kittisak Thotsaporn

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, 106(26), 10603-10608
Proceedings of the National Academy of Sciences of the United States of America 106 (2009) 26
Proceedings of the National Academy of Sciences of the United States of America, 106(26), 10603-10608. NATL ACAD SCIENCES

Dioxygen (O 2 ) and other gas molecules have a fundamental role in a variety of enzymatic reactions. However, it is only poorly understood which O 2 uptake mechanism enzymes employ to promote efficient catalysis and how general this is. We investigat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4691200f37547004333d0e3268c9d33e
https://doi.org/10.1073/pnas.0903809106

Control of C4a-hydroperoxyflavin protonation in the oxygenase component of p-hydroxyphenylacetate-3-hydroxylase

Autor: Pirom Chenprakhon, Jeerus Sucharitakul, Duangthip Trisrivirat, Kittisak Thotsaporn, Pimchai Chaiyen

Publikováno v: Biochemistry. 53(25)

The protonation status of the peroxide moiety in C4a-(hydro)peroxyflavin of p-hydroxyphenylacetate-3-hydroxylase can be directly monitored using transient kinetics. The pKa for the wild-type (WT) enzyme is 9.8 ± 0.2, while the values for the H396N,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e39db4661509e659e1240087f5a9c647
https://pubmed.ncbi.nlm.nih.gov/24878148