Bacillus megaterium has both a functional BluB protein required for DMB synthesis and a related flavoprotein that forms a stable radical species.

Autor: Hannah F Collins, Rebekka Biedendieck, Helen K Leech, Michael Gray, Jorge C Escalante-Semerena, Kirsty J McLean, Andrew W Munro, Stephen E J Rigby, Martin J Warren, Andrew D Lawrence

Publikováno v: PLoS ONE, Vol 8, Iss 2, p e55708 (2013)

Despite the extensive study of the biosynthesis of the complex molecule B12 (cobalamin), the mechanism by which the lower ligand 5,6-dimethylbenzimidazole (DMB) is formed has remained something of a mystery. However, recent work has identified and ch

Externí odkaz: https://doaj.org/article/bfd765d7e8924914a5f57c87c6186900

Design and Synthesis of Imidazole and Triazole Pyrazoles as Mycobacterium Tuberculosis CYP121A1 Inhibitors

Autor: Dr. Safaa M. Kishk, Dr. Kirsty J. McLean, Dr. Sakshi Sood, Darren Smith, Jack W.D. Evans, Prof. Mohamed A. Helal, Prof. Mohamed S. Gomaa, Prof. Ismail Salama, Prof. Samia M. Mostafa, Dr. Luiz Pedro S. de Carvalho, Colin W. Levy, Prof. Andrew W. Munro, Dr. Claire Simons

Publikováno v: ChemistryOpen, Vol 8, Iss 7, Pp 995-1011 (2019)

Abstract The emergence of untreatable drug‐resistant strains of Mycobacterium tuberculosis is a major public health problem worldwide, and the identification of new efficient treatments is urgently needed. Mycobacterium tuberculosis cytochrome P450

Externí odkaz: https://doaj.org/article/3aae741715394bd2a5f6041ac6a202cf

Expression, Purification, and Biochemical Characterization of the Flavocytochrome P450 CYP505A30 from Myceliophthora thermophila

Autor: George J. Baker, Hazel M. Girvan, Sarah Matthews, Kirsty J. McLean, Marina Golovanova, Timothy N. Waltham, Stephen E. J. Rigby, David R. Nelson, Richard T. Blankley, Andrew W. Munro

Publikováno v: ACS Omega, Vol 2, Iss 8, Pp 4705-4724 (2017)

Externí odkaz: https://doaj.org/article/9d2e711fc5a24b71bb75ed933c0f3c61

A new strategy for hit generation: Novel in cellulo active inhibitors of CYP121A1 from Mycobacterium tuberculosis via a combined X-ray crystallographic and phenotypic screening approach (XP screen)

Autor: Martyn Frederickson, Irwin R. Selvam, Dimitrios Evangelopoulos, Kirsty J. McLean, Mona M. Katariya, Richard B. Tunnicliffe, Bethany Campbell, Madeline E. Kavanagh, Sitthivut Charoensutthivarakul, Richard T. Blankley, Colin W. Levy, Luiz Pedro S. de Carvalho, David Leys, Andrew W. Munro, Anthony G. Coyne, Chris Abell

Publikováno v: Frederickson, M, Selvam, I R, Evangelopoulos, D, McLean, K J, Katariya, M M, Tunnicliffe, R B, Campbell, B, Kavanagh, M E, Charoensutthivarakul, S, Blankley, R T, Levy, C W, de Carvalho, L P S, Leys, D, Munro, A W, Coyne, A G & Abell, C 2022, ' A new strategy for hit generation: Novel in cellulo active inhibitors of CYP121A1 from Mycobacterium tuberculosis via a combined X-ray crystallographic and phenotypic screening approach (XP screen) ', European Journal of Medicinal Chemistry, vol. 230, 114105 . https://doi.org/10.1016/j.ejmech.2022.114105

There is a pressing need for new drugs against tuberculosis (TB) to combat the growing resistance to current antituberculars. Herein a novel strategy is described for hit generation against promising TB targets involving X-ray crystallographic screen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6d9c2705833bafc4766f5cadaceb345

Structure–Activity Relationships of cyclo(l-Tyrosyl-l-tyrosine) Derivatives Binding to Mycobacterium tuberculosis CYP121: Iodinated Analogues Promote Shift to High-Spin Adduct

Autor: Craig A. Hutton, Harshwardhan Poddar, James A. Triccas, Kirsty J. McLean, Gayathri Nagalingam, Andrew W. Munro, Sunnia Rajput, Irwin Selvam, Colin Levy

Publikováno v: Journal of Medicinal Chemistry. 62:9792-9805

A series of analogues of cyclo(l-tyrosyl-l-tyrosine), the substrate of the Mycobacterium tuberculosis enzyme CYP121, have been synthesized and analyzed by UV-vis and electron paramagnetic resonance spectroscopy and by X-ray crystallography. The intro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e010e8cb9dc0178b58f8c2f260ea7a96
https://doi.org/10.1021/acs.jmedchem.9b01199