Active site substitution a82w improves the regioselectivity of steroid hydroxylation by cytochrome p450 bm3 mutants as rationalized by spin relaxation nuclear magnetic resonance studies

Autor: Sybren S. Wijmenga, Eduardo Vottero, Vanina Rea, Ard J. Kolkman, Nico P. E. Vermeulen, Kirsten A.M. Ampt, Jan N. M. Commandeur, E.J. Stronks, Maarten Honing

Publikováno v: Biochemistry, 51, 750-760
Biochemistry, 51(3), 750-760. American Chemical Society
Rea, V, Kolkman, A J, Vottero, E R, Stronks, E J, Ampt, K A, Honing, M, Vermeulen, N P E, Wijmenga, S S & Commandeur, J N M 2012, ' Active Site Substitution A82W Improves the Regioselectivity of Steroid Hydroxylation by Cytochrome P450 BM3 Mutants As Rationalized by Spin Relaxation Nuclear Magnetic Resonance Studies ', Biochemistry, vol. 51, no. 3, pp. 750-760 . https://doi.org/10.1021/bi201433h
Biochemistry, 51, 3, pp. 750-760

Cytochrome P450 BM3 from Bacillus megaterium is a monooxygenase with great potential for biotechnological applications. In this paper, we present engineered drug-metabolizing P450 BM3 mutants as a novel tool for regioselective hydroxylation of steroi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6516bb58e7de7294bb79663245d3cb7
https://doi.org/10.1021/bi201433h

The Unstable Part of the Apical Stem of Duck Hepatitis B Virus Epsilon Shows Enhanced Base Pair Opening but Not Pico- to Nanosecond Dynamics and Is Essential for Reverse Transcriptase Binding

Autor: Marco Tessari, Frank H. T. Nelissen, R. M. van der Werf, Kirsten A.M. Ampt, Sybren S. Wijmenga

Publikováno v: Biochemistry, 48, 10499-10508
Biochemistry, 48, 44, pp. 10499-10508

Hepatitis B virus (HBV) replication starts with binding of reverse transcriptase (RT) to the apical stem-loop region of epsilon, a conserved element of the RNA pregenome. For duck HBV, an in vitro replication system has provided molecular details of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03d8bccacb219b337ad67bba91ab99d9
https://doi.org/10.1021/bi9011385

Determination of size and sign of hetero-nuclear coupling constants from 2d f-19-c-13 correlation spectra

Autor: Sybren S. Wijmenga, Peter Dvortsak, Martin Jaeger, Ramon M. van der Werf, Ruud L. E. G. Aspers, Kirsten A.M. Ampt

Publikováno v: Journal of Magnetic Resonance, 215, 27-33
Journal of Magnetic Resonance, 215, 27-33. Academic Press
Journal of Magnetic Resonance, 215, pp. 27-33

Fluorinated organic compounds have become increasingly important within the polymer and the pharmaceutical industry as well as for clinical applications. For the structural elucidation of such compounds, NMR experiments with fluorine detection are of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::395d09e8761b841b7f6c61c1684b40eb
http://hdl.handle.net/2066/103291

Simultaneous analysis of plasma and CSF by NMR and hierarchical models fusion

Autor: Paul J. Michiels, Tinka Tuinstra, Joram M. Posma, Sybren S. Wijmenga, Frederic C. Girard, Lutgarde M. C. Buydens, Lionel Blanchet, Theo M. Luider, Agnieszka Smolinska, Amos Attali, Kirsten A.M. Ampt, Marek Doskocz

Publikováno v: Analytical and Bioanalytical Chemistry, 403, 4, pp. 947-959
Analytical and Bioanalytical Chemistry, 403, 947-959
Analytical and Bioanalytical Chemistry, 403(4), 947-59. Springer
Analytical and Bioanalytical Chemistry, 403(4), 947-959. Springer-Verlag
Analytical and Bioanalytical Chemistry

Because cerebrospinal fluid (CSF) is the biofluid which interacts most closely with the central nervous system, it holds promise as a reporter of neurological disease, for example multiple sclerosis (MScl). To characterize the metabolomics profile of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc6204719554ec203c4d50a1fb26807e
https://hdl.handle.net/2066/1837662066/103762

Fusion of metabolomics and proteomics data for biomarkers discovery: Case study on the experimental autoimmune encephalomyelitis

Autor: Theo M. Luider, Hans van Aken, Kirsten A.M. Ampt, Marcel P. Stoop, Tinka Tuinstra, Lionel Blanchet, Amos Attali, Agnieszka Smolinska, Ernst Suidgeest, Lutgarde M. C. Buydens, Sybren S. Wijmenga

Publikováno v: BMC Bioinformatics, Vol 12, Iss 1, p 254 (2011)
BMC Bioinformatics, 12
BMC Bioinformatics, 12. BioMed Central Ltd.
BMC Bioinformatics

Background Analysis of Cerebrospinal Fluid (CSF) samples holds great promise to diagnose neurological pathologies and gain insight into the molecular background of these pathologies. Proteomics and metabolomics methods provide invaluable information

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f95ff536a0c5f772b93269c42759b8dd
https://hdl.handle.net/https://repository.ubn.ru.nl/handle/2066/91842