Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Kirk M. Brown"'
Autor:
Kirk M. Brown, Jayaprakash K. Nair, Maja M. Janas, Yesseinia I. Anglero-Rodriguez, Lan T. H. Dang, Haiyan Peng, Christopher S. Theile, Elena Castellanos-Rizaldos, Christopher Brown, Donald Foster, Jeffrey Kurz, Jeffrey Allen, Rajanikanth Maganti, Jing Li, Shigeo Matsuda, Matthew Stricos, Tyler Chickering, Michelle Jung, Kelly Wassarman, Jeff Rollins, Lauren Woods, Alex Kelin, Dale C. Guenther, Melissa W. Mobley, John Petrulis, Robin McDougall, Timothy Racie, Jessica Bombardier, Diana Cha, Saket Agarwal, Lei Johnson, Yongfeng Jiang, Scott Lentini, Jason Gilbert, Tuyen Nguyen, Samantha Chigas, Sarah LeBlanc, Urjana Poreci, Anne Kasper, Arlin B. Rogers, Saeho Chong, Wendell Davis, Jessica E. Sutherland, Adam Castoreno, Stuart Milstein, Mark K. Schlegel, Ivan Zlatev, Klaus Charisse, Mark Keating, Muthiah Manoharan, Kevin Fitzgerald, Jing-Tao Wu, Martin A. Maier, Vasant Jadhav
Publikováno v:
Nature biotechnology. 40(10)
Therapeutics based on short interfering RNAs (siRNAs) delivered to hepatocytes have been approved, but new delivery solutions are needed to target additional organs. Here we show that conjugation of 2'-O-hexadecyl (C16) to siRNAs enables safe, potent
Autor:
Kirk M. Brown, Grant A. Bateman
Publikováno v:
Child's Nervous System. 28:55-63
Despite 100 years of study, the theories of cerebrospinal fluid (CSF) formation and absorption remain controversial. Measuring CSF flow through the aqueduct using magnetic resonance imaging (MRI) provides a unique insight into the physiology of CSF h
Publikováno v:
Genes & Development. 19:1315-1327
The process of mRNA 3′ end formation is not simply a perfunctory step in eukaryotic gene expression. At least one-half of all human genes are subject to alternative 3′ processing (Iseli et al. 2002), the consequences of which may impact the prote
Autor:
Gregory M. Gilmartin, Kirk M. Brown
Publikováno v:
Molecular Cell. 12(6):1467-1476
Human cleavage factor I(m) (CFI(m)) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFI(m) can stimulate both cleavage and poly(A) addit
Publikováno v:
International Journal of Biological Macromolecules. 31:29-35
A procedure is described for the preparation of large amounts of guar galactomannan by acid hydrolysis that yields samples of various molecular weights (MW) with uniform polydispersity. This contrasts with preparation by enzymatic degradation that yi
Publikováno v:
Biomacromolecules. 3:456-461
Aqueous solutions of guar galactomannan and hydroxypropyl guars (HPG) with different molar substitution (MS) levels were studied using dilute solution viscometry and gel permeation chromatography. When guar is modified to HPG, the added hydroxypropyl
Autor:
Hong Cao, Kirk M Brown, Chia-Ying Chu, Chimmanamada U. Dinesh, Ya-Lin Chiu, Akbar Ali, Tariq M. Rana
Publikováno v:
Chemistrybiology. 12(6)
SummaryRNA interference (RNAi) is a process whereby short-interfering RNAs (siRNA) silence gene expression in a sequence-specific manner. We have screened a chemical library of substituted dihydropteridinones and identified a nontoxic, cell permeable
Publikováno v:
Nature structuralmolecular biology. 12(5)
In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when targe
Publikováno v:
Nature structuralmolecular biology. 12(2)
RNA interference (RNAi) has become a research tool to control gene expression in various organisms and holds potential as a new therapeutic strategy. The mechanism of small interfering RNA (siRNA)-mediated RNAi involves target mRNA cleavage and destr
Publikováno v:
The EMBO journal. 17(8)
The complex formed by the hairpin ribozyme and its substrate consists of two independently folding domains which interact to form a catalytic structure. Fluorescence resonance energy transfer methods permit us to study reversible transitions of the c