Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Kirankumar Mysore"'
Engineering Agrobacterium tumefaciens with a Type III Secretion System to Express Type III Effectors
Autor:
Vidhyavathi Raman, Kirankumar Mysore
Publikováno v:
Bio-Protocol, Vol 13, Iss 15 (2023)
Plants elicit defense responses when exposed to pathogens, which partly contribute to the resistance of plants to Agrobacterium tumefaciens–mediated transformation. Some pathogenic bacteria have sophisticated mechanisms to counteract these defense
Externí odkaz:
https://doaj.org/article/848c61327137411795bda3f90c74cb89
Publikováno v:
Bio-Protocol, Vol 11, Iss 19 (2021)
Dark respiration refers to experimental measures of leaf respiration in the absence of light, done to distinguish it from the photorespiration that occurs during photosynthesis. Dark aerobic respiration reactions occur solely in the mitochondria and
Externí odkaz:
https://doaj.org/article/012bcd27b2724968b119d253e9f02b78
Publikováno v:
Bio-Protocol, Vol 10, Iss 15 (2020)
ATPases are the enzymes that breakdown ATP to ADP and release inorganic phosphate (Pi). Here we provide a detailed protocol to determine the ATPase activity of a recombinant AAA+-ATPase protein (GENERAL CONTROL NON-REPRESSIBLE-4 [GCN4]) by spectropho
Externí odkaz:
https://doaj.org/article/1786d40cf3774d8fa32edc16136af673
Publikováno v:
Bio-Protocol, Vol 7, Iss 2 (2017)
Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on Arabidopsis thaliana. Here, we demonstrate a rapid and reliable flood-inocul
Externí odkaz:
https://doaj.org/article/ef99aaaf93df47199a9fc018feeb5149
Publikováno v:
Bio-Protocol, Vol 2, Iss 20 (2012)
NADPH oxidase is a membrane-bound enzyme that generates (O2-) by transferring electrons from NADPH to molecular oxygen O2. O2-is spontaneously dismasted to the more stable form H2O2. Both O2-and H2O2 are forms ofreactive oxygen species (ROS), which a
Externí odkaz:
https://doaj.org/article/406bf232b9aa498badfb69bb61179f90
Publikováno v:
Bio-Protocol, Vol 2, Iss 20 (2012)
Glycolate oxidase is located in the peroxisome and is involved in the photorespiratory cycle which recovers some of the carbon loss during photosynthesis. Glycolate oxidase converts glycolate to glyxoylate with the concomitant production of H2O2.In t
Externí odkaz:
https://doaj.org/article/88561a668e294e789fe5c1860e92dce0
Akademický článek
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Publikováno v:
Fundamental Research. 3:219-224
Publikováno v:
Plant Molecular Biology Reporter. 39:773-791
Agrobacterium tumefaciens is a unique pathogen with the ability to transfer a portion of its DNA, the T-DNA, to other organisms. The role of DNA repair genes in Agrobacterium transformation remains controversial. In order to understand if the host DN
Autor:
Parwinder, Kaur, Christopher, Lui, Olga, Dudchenko, Raja Sekhar, Nandety, Bhavna, Hurgobin, Melanie, Pham, Erez, Lieberman Aiden, Jiangqi, Wen, Kirankumar, Mysore
Publikováno v:
International Journal of Molecular Sciences
Legumes are of great interest for sustainable agricultural production as they fix atmospheric nitrogen to improve the soil. Medicago truncatula is a well-established model legume, and extensive studies in fundamental molecular, physiological, and dev