Molecular basis of the final step of cell division in Streptococcus pneumoniae

Autor: Siseth Martínez-Caballero, Céline Freton, Rafael Molina, Sergio G. Bartual, Virginie Gueguen-Chaignon, Chryslène Mercy, Federico Gago, Kiran V. Mahasenan, Inés G. Muñoz, Mijoon Lee, Dusan Hesek, Shahriar Mobashery, Juan A. Hermoso, Christophe Grangeasse

Publikováno v: Cell Reports, Vol 42, Iss 7, Pp 112756- (2023)

Summary: Bacterial cell-wall hydrolases must be tightly regulated during bacterial cell division to prevent aberrant cell lysis and to allow final separation of viable daughter cells. In a multidisciplinary work, we disclose the molecular dialogue be

Externí odkaz: https://doaj.org/article/d81c8fc5e2aa4f5d9ff7f86ec5f0dc25

Integrative structural biology of the penicillin-binding protein-1 from Staphylococcus aureus, an essential component of the divisome machinery

Autor: Siseth Martínez-Caballero, Kiran V. Mahasenan, Choon Kim, Rafael Molina, Rhona Feltzer, Mijoon Lee, Renee Bouley, Dusan Hesek, Jed F. Fisher, Inés G. Muñoz, Mayland Chang, Shahriar Mobashery, Juan A. Hermoso

Publikováno v: Computational and Structural Biotechnology Journal, Vol 19, Iss , Pp 5392-5405 (2021)

The penicillin-binding proteins are the enzyme catalysts of the critical transpeptidation crosslinking polymerization reaction of bacterial peptidoglycan synthesis and the molecular targets of the penicillin antibiotics. Here, we report a combined cr

Externí odkaz: https://doaj.org/article/0af59740be1241078a1417fa0b394874

Production of Proteins of the SARS-CoV‑2 Proteome for Drug Discovery

Autor: Atul Bhardwaj, Kiran V. Mahasenan, Choon Kim, Olaf Wiest, Mayland Chang, Shahriar Mobashery

Publikováno v: ACS Omega, Vol 6, Iss 30, Pp 19983-19994 (2021)
ACS Omega

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the causative agent of the coronavirus disease of 2019 (COVID-19). Its genome encodes two open reading frames for two large proteins, PP1a and PP1ab. Within the two polypeptide stret

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::714241b67153764f53133ee2de17e1e6
https://doaj.org/article/7da29ff0ab894a20996c30c25f9d7507

Catalytic Cycle of Glycoside Hydrolase BglX from Pseudomonas aeruginosa and Its Implications for Biofilm Formation

Autor: Julia Sanz-Aparicio, Mijoon Lee, Kiran V. Mahasenan, María T. Batuecas, Shahriar Mobashery, Choon Kim, Juan A. Hermoso, Jed F. Fisher, Neha Rana, Stefania De Benedetti, Dusan Hesek

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
instname

8 pags., 5 figs.
BglX is a heretofore uncharacterized periplasmic glycoside hydrolase (GH) of the human pathogen Pseudomonas aeruginosa. X-ray analysis identifies it as a protein homodimer. The two active sites of the homodimer comprise catalyti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23fb3588b071701ca8769f50964297a2
https://doi.org/10.1021/acschembio.9b00754

Integrative structural biology of the penicillin-binding protein-1 from Staphylococcus aureus, an essential component of the divisome machinery

Autor: Kiran V. Mahasenan, Rhona Feltzer, Dusan Hesek, Mijoon Lee, Renee Bouley, Siseth Martínez-Caballero, Choon Kim, Jed F. Fisher, Shahriar Mobashery, Rafael Molina, Juan A. Hermoso, Inés G. Muñoz, Mayland Chang

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
instname
Computational and Structural Biotechnology Journal
Computational and Structural Biotechnology Journal, Vol 19, Iss, Pp 5392-5405 (2021)

Graphical abstract
Highlights • X-ray crystallography, SAXS and MD simulations elucidates the 3D structure for saPBP1. • 3D structure of saPBP1 reveals dynamical mobility for the pedestal and PASTA domains. • Structures with β-lactams and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa14893e10e00bda2814c5622751d7fd
http://hdl.handle.net/10261/256139