Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling.

Autor: Fontana J; Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, WA, USA.; Department of Chemistry, University of Washington, Seattle, WA, USA.; Department of Chemical Engineering, University of Washington, Seattle, WA, USA., Sparkman-Yager D; Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, WA, USA.; Department of Chemical Engineering, University of Washington, Seattle, WA, USA., Faulkner I; Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, WA, USA.; Department of Chemical Engineering, University of Washington, Seattle, WA, USA., Cardiff R; Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, WA, USA.; Department of Chemistry, University of Washington, Seattle, WA, USA.; Department of Chemical Engineering, University of Washington, Seattle, WA, USA., Kiattisewee C; Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, WA, USA.; Department of Chemistry, University of Washington, Seattle, WA, USA.; Department of Chemical Engineering, University of Washington, Seattle, WA, USA., Walls A; Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, WA, USA.; Department of Chemical Engineering, University of Washington, Seattle, WA, USA., Primo TG; Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, WA, USA.; Department of Bioengineering, University of Washington, Seattle, WA, USA., Kinnunen PC; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Biofuels and Bioproducts Division, DOE Joint BioEnergy Institute, Emeryville, CA, USA.; DOE Agile BioFoundry, Emeryville, CA, USA., Garcia Martin H; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Biofuels and Bioproducts Division, DOE Joint BioEnergy Institute, Emeryville, CA, USA.; DOE Agile BioFoundry, Emeryville, CA, USA., Zalatan JG; Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, WA, USA. zalatan@uw.edu.; Department of Chemistry, University of Washington, Seattle, WA, USA. zalatan@uw.edu., Carothers JM; Molecular Engineering & Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, WA, USA. jcaroth@uw.edu.; Department of Chemical Engineering, University of Washington, Seattle, WA, USA. jcaroth@uw.edu.

Publikováno v: Nature communications [Nat Commun] 2024 Jul 27; Vol. 15 (1), pp. 6341. Date of Electronic Publication: 2024 Jul 27.

Oscillatory ERK Signaling and Morphology Determine Heterogeneity of Breast Cancer Cell Chemotaxis via MEK-ERK and p38-MAPK Signaling Pathways.

Autor: Ho KKY; Department of Radiology, University of Michigan, Ann Arbor, MI 48109, USA., Srivastava S; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA., Kinnunen PC; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA., Garikipati K; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Mathematics, University of Michigan, Ann Arbor, MI 48109, USA.; Michigan Institute for Computational Discovery & Engineering, University of Michigan, Ann Arbor, MI 48109, USA., Luker GD; Department of Radiology, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA., Luker KE; Department of Radiology, University of Michigan, Ann Arbor, MI 48109, USA.; Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA.

Publikováno v: Bioengineering (Basel, Switzerland) [Bioengineering (Basel)] 2023 Feb 18; Vol. 10 (2). Date of Electronic Publication: 2023 Feb 18.

Co-optimization of therapeutic antibody affinity and specificity using machine learning models that generalize to novel mutational space.

Autor: Makowski EK; Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI, 48109, USA.; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA., Kinnunen PC; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA., Huang J; Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI, 48109, USA.; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA., Wu L; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA.; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA., Smith MD; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA.; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA., Wang T; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA.; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA., Desai AA; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA.; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA., Streu CN; Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI, 48109, USA.; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA.; Department of Chemistry, Albion College, Albion, MI, 49224, USA., Zhang Y; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA.; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA., Zupancic JM; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA.; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA., Schardt JS; Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI, 48109, USA.; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA.; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA., Linderman JJ; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA., Tessier PM; Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI, 48109, USA. ptessier@umich.edu.; Biointerfaces Institute, University of Michigan, Ann Arbor, MI, 48109, USA. ptessier@umich.edu.; Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA. ptessier@umich.edu.; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA. ptessier@umich.edu.

Publikováno v: Nature communications [Nat Commun] 2022 Jul 01; Vol. 13 (1), pp. 3788. Date of Electronic Publication: 2022 Jul 01.

Pre-existing Cell States Control Heterogeneity of Both EGFR and CXCR4 Signaling.

Autor: Spinosa PC; Department of Chemical Engineering, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI 48109-2800 USA., Kinnunen PC; Department of Chemical Engineering, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI 48109-2800 USA., Humphries BA; Department of Radiology Center for Molecular Imaging, University of Michigan Medical School, Ann Arbor, MI 48109 USA., Luker GD; Department of Radiology Center for Molecular Imaging, University of Michigan Medical School, Ann Arbor, MI 48109 USA.; Department of Biomedical Engineering, University of Michigan Medical School, Ann Arbor, MI USA 48109.; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI USA 48109., Luker KE; Department of Radiology Center for Molecular Imaging, University of Michigan Medical School, Ann Arbor, MI 48109 USA.; Department of Radiology, Center for Molecular Imaging, University of Michigan, 109 Zina Pitcher Place, A526 BSRB, Ann Arbor, MI 48109-2200 USA., Linderman JJ; Department of Chemical Engineering, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI 48109-2800 USA.; Department of Biomedical Engineering, University of Michigan Medical School, Ann Arbor, MI USA 48109.

Publikováno v: Cellular and molecular bioengineering [Cell Mol Bioeng] 2020 Jul 27; Vol. 14 (1), pp. 49-64. Date of Electronic Publication: 2020 Jul 27 (Print Publication: 2021).