Negative DNA supercoiling induces genome-wide Cas9 off-target activity

Autor: Newton, Matthew D., Losito, Marialucrezia, Smith, Quentin M., Parnandi, Nishita, Taylor, Benjamin J., Akcakaya, Pinar, Maresca, Marcello, van Eijk, Patrick, Reed, Simon H., Boulton, Simon J., King, Graeme A., Cuomo, Maria Emanuela, Rueda, David S.

Publikováno v: In Molecular Cell 5 October 2023 83(19):3533-3545

DNA twist stability changes with magnesium(2+) concentration

Autor: Broekmans, Onno D., King, Graeme A., Stephens, Greg J., Wuite, Gijs J. L.

Publikováno v: Phys. Rev. Lett. 116, 258102 (2016)

For an understanding of DNA elasticity at high mechanical loads (F > 30 pN), its helical nature needs to be taken into account, in the form of coupling between the twist and stretch degrees of freedom. The prevailing model for DNA elasticity, the wor

Externí odkaz: http://arxiv.org/abs/1412.8753

Sugar Regulation of Harvest-Related Genes in Asparagus

Autor: King, Graeme A.

Publikováno v: Plant Physiology, 1996 Jul 01. 111(3), 877-883.

Externí odkaz: https://www.jstor.org/stable/4277235

Constructing arrays of nucleosome positioning sequences using Gibson Assembly for single-molecule studies.

Autor: Spakman, Dian¹, King, Graeme A.^1,2 g.king@ucl.ac.uk, Peterman, Erwin J. G.¹ e.j.g.peterman@vu.nl, Wuite, Gijs J. L.¹ g.j.l.wuite@vu.nl

Publikováno v: Scientific Reports. 6/18/2020, Vol. 10 Issue 1, p1-11. 11p.

Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers

Autor: King, Graeme A., Spakman, Dian, Peterman, Erwin J.G., Wuite, Gijs J.L., Gennerich, Arne

Publikováno v: Optical Tweezers ISBN: 9781071622285
King, G A, Spakman, D, Peterman, E J G & Wuite, G J L 2022, Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers . in A Gennerich (ed.), Optical Tweezers : Methods and Protocols . 2nd edn, Methods in Molecular Biology, vol. 2478, Humana Press Inc, pp. 243-272 . https://doi.org/10.1007/978-1-0716-2229-2_9
Optical Tweezers: Methods and Protocols, 243-272
ISSUE=2nd;STARTPAGE=243;ENDPAGE=272;TITLE=Optical Tweezers

Many genomic processes lead to the formation of underwound (negatively supercoiled) or overwound (positively supercoiled) DNA. These DNA topological changes regulate the interactions of DNA-binding proteins, including transcription factors, architect

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0cbb59537a68ff4060ae37304bb7ffd
https://doi.org/10.1007/978-1-0716-2229-2_9