Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Kimberly Trabbic-Carlson"'
Autor:
Mohammed F. Shamji, Trine Christensen, Sue Dagher, Kimberly Trabbic-Carlson, Miriam Amiram, Ashutosh Chilkoti, Lori A. Setton
Publikováno v:
Protein Science. 18:1377-1387
We have previously developed a method to purify recombinant proteins, termed inverse transition cycling (ITC) that eliminates the need for column chromatography. ITC exploits the inverse solubility phase transition of an elastin-like polypeptide (ELP
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0c01276af4172d8530c7585595c0acc6
https://doi.org/10.1002/pro.157
https://doi.org/10.1002/pro.157
Publikováno v:
Biomacromolecules. 8:1417-1424
This paper reports an improvement in the purification of thioredoxin (Trx) expressed from E. coli by inverse transition cycling (ITC) using cationic elastin-like polypeptides (ELPs). Two ELP libraries having 2% and 5% lysine residues and molecular we
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd47195838c6231765d7dac301627005
https://doi.org/10.1021/bm060849t
https://doi.org/10.1021/bm060849t
Autor:
Fernando Albertorio, Paul S. Cremer, Kimberly Trabbic-Carlson, Yanjie Zhang, and Ashutosh Chilkoti
Publikováno v:
Biomacromolecules. 7:2192-2199
The kinetics of aqueous two-phase system (ATPS) formation for elastin-like polypeptides (ELP) with defined chemical composition and chain length was investigated by dark field microscopy in an on-chip format with a linear temperature gradient. Scatte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ebba6950a211c8233872aa843fa53c3
https://doi.org/10.1021/bm060254y
https://doi.org/10.1021/bm060254y
Publikováno v:
Biotechnology Progress. 22:638-646
Elastin-like polypeptides (ELPs) are recombinant peptide-based biopolymers that contain repetitive sequences enriched in glycine, valine, proline, and alanine. Because of the unusually large fraction of these amino acids in ELPs as compared to other
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb823466b283bce9da1bb142ab1a8089
https://doi.org/10.1021/bp0503742
https://doi.org/10.1021/bp0503742
Autor:
Shin R. Ong, Dana L. Nettles, Ashutosh Chilkoti, Kimberly Trabbic-Carlson, Dong W. Lim, Lori A. Setton
Publikováno v:
Biomaterials. 27:1930-1935
Elastin-like polypeptides (ELPs) are a class of biocompatible, non-immunogenic and crosslinkable biomaterials that offer promise for use as an injectable scaffold for cartilage repair. In this study, an oligohistidine (His(6)) epitope tag was incorpo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d0d99a7b048e8172992c81bea383755
https://doi.org/10.1016/j.biomaterials.2005.10.018
https://doi.org/10.1016/j.biomaterials.2005.10.018
Autor:
Kimberly Trabbic-Carlson, Ashutosh Chilkoti, Dan E. Meyer, Nidhi Nath, Thomas H. LaBean, Ronald T. Piervincenzi, L. Liu
Publikováno v:
Protein Engineering Design and Selection. 17:57-66
The limited throughput, scalability and high cost of protein purification by chromatography provide motivation for the development of non-chromatographic protein purification technologies that are cheaper and easier to implement in a high-throughput
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::324178f35ba4809e4b204f9f2a1f6795
https://doi.org/10.1093/protein/gzh006
https://doi.org/10.1093/protein/gzh006
Publikováno v:
Analytical Biochemistry. 360:166-168
A crucial and challenging problem in proteomics is purification, identification, and characterization of proteins, some of which are expressed at very low levels. The preferred method for purification of low abundance proteins exploits multiple affin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3b3f9b620a14598b0b133c0fa977e6d
https://doi.org/10.1016/j.ab.2006.09.020
https://doi.org/10.1016/j.ab.2006.09.020
Publikováno v:
Biointerphases. 3(3)
The conformational changes in elastinlike polypeptides (ELPs) grafted to a solid/solution interface via different architectures were studied using surface plasmon resonance spectroscopy and surface plasmon field-enhanced fluorescence spectroscopy (SP
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96a1cc0ab810dcc353559e94cb77b1a2
https://pubmed.ncbi.nlm.nih.gov/20408702
https://pubmed.ncbi.nlm.nih.gov/20408702
Autor:
Trine, Christensen, Miriam, Amiram, Sue, Dagher, Kimberly, Trabbic-Carlson, Mohammed F, Shamji, Lori A, Setton, Ashutosh, Chilkoti
Publikováno v:
Protein science : a publication of the Protein Society. 18(7)
We have previously developed a method to purify recombinant proteins, termed inverse transition cycling (ITC) that eliminates the need for column chromatography. ITC exploits the inverse solubility phase transition of an elastin-like polypeptide (ELP
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::695d6178d0b3677130f90ca7424a2d2a
https://pubmed.ncbi.nlm.nih.gov/19533768
https://pubmed.ncbi.nlm.nih.gov/19533768
Publikováno v:
Biotechnology and bioengineering. 95(3)
This article describes a simple and potentially scalable microfiltration method for purification of recombinant proteins. This method is based on the fact that when an elastin-like polypeptide (ELP) is fused to a target protein, the inverse phase tra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::335c173f6613e00292c10c7fb0a22c49
https://pubmed.ncbi.nlm.nih.gov/16767781
https://pubmed.ncbi.nlm.nih.gov/16767781