Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Kimberly L. Crawford"'
Direct quantification of gene expression in homogenates of formalin-fixed, paraffin-embedded tissues
Autor:
Wen Yang, Botoul Maqsodi, Yunqing Ma, Son Bui, Kimberly L. Crawford, Gary K. McMaster, Frank Witney, Yuling Luo
Publikováno v:
BioTechniques, Vol 40, Iss 4, Pp 481-486 (2006)
Formalin-fixed, paraffin-embedded (FFPE) tissues represent an important source of archival materials for gene expression profiling. We report here the development of a modified branch DNA assay that allows direct quantification of messenger RNA (mRNA
Externí odkaz:
https://doaj.org/article/cf540442398a432da1f5b496c67af658
Direct quantification of gene expression in homogenates of formalin-fixed, paraffin-embedded tissues
Autor:
Son Bui, Yunqing Ma, Wen Yang, Frank Witney, Yuling Luo, Botoul Maqsodi, Kimberly L. Crawford, Gary K. McMaster
Publikováno v:
BioTechniques. 40:481-486
Formalin-fixed, paraffin-embedded (FFPE) tissues represent an important source of archival materials for gene expression profiling. We report here the development of a modified branch DNA assay that allows direct quantification of messenger RNA (mRNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2867b89afbe92203fc42a552ea351c8
https://doi.org/10.2144/000112133
https://doi.org/10.2144/000112133
Publikováno v:
The Journal of Physical Chemistry. 97:3058-3066
A thermodynamic basis is derived and experimentally verified for predicting the temperature-dependent spectral changes in solutions containing a pH indicator and any number of pH-buffering components. Absorbance by the acidic and basic species of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::805ede18f0da5f3a5c0493d0fe059c22
https://doi.org/10.1021/j100114a037
https://doi.org/10.1021/j100114a037
Autor:
Yunqing Ma, Michael Flagella, Aiguo Zhang, Wen Yang, Frank Witney, Zhi Zheng, Gary K. McMaster, Yuling Luo, Larry Pastor, Kimberly L. Crawford, Cung Tuong Nguyen, Son Bui
Publikováno v:
Analytical biochemistry. 352(1)
We describe a novel method to quantitatively measure messenger RNA (mRNA) expression of multiple genes directly from crude cell lysates and tissue homogenates without the need for RNA purification or target amplification. The multiplex branched DNA (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70bcacfa049c050399e1a1c3a16f822b
https://pubmed.ncbi.nlm.nih.gov/16545767
https://pubmed.ncbi.nlm.nih.gov/16545767