Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Kimberly D. Insigne"'
Autor:
Timothy C. Yu, Winnie L. Liu, Marcia S. Brinck, Jessica E. Davis, Jeremy Shek, Grace Bower, Tal Einav, Kimberly D. Insigne, Rob Phillips, Sriram Kosuri, Guillaume Urtecho
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Precisely tuning the genetic response to environmental stimuli is a key step in engineering synthetic biology systems. Here, the authors profile 8269 IPTG-induced promoters to deconstruct the relationship between sequence architecture and gene expres
Externí odkaz:
https://doaj.org/article/4cab2046d5594f268114bd03aed9a468
Autor:
Jessica E. Davis, Rob Phillips, Jeremy Shek, Timothy C. Yu, Winnie L. Liu, Marcia Brinck, Grace Bower, Guillaume Urtecho, Tal Einav, Sriram Kosuri, Kimberly D. Insigne
Publikováno v:
Nature Communications
Nature communications, vol 12, iss 1
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Nature communications, vol 12, iss 1
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
A crucial step towards engineering biological systems is the ability to precisely tune the genetic response to environmental stimuli. In the case of Escherichia coli inducible promoters, our incomplete understanding of the relationship between sequen
Autor:
Arielle D. Tripp, Tracey Chan, Sriram Kosuri, Nathan B. Lubock, Hwang-Beom Kim, Guillaume Urtecho, Marcia Brinck, Kimberly D. Insigne
SummaryDespite decades of intense genetic, biochemical, and evolutionary characterizations of bacterial promoters, we still lack the basic ability to identify or predict transcriptional activities of promoters using primary sequence. Even in simple,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ea06ddff50f1f6bba07c0c2430ef2fa
Autor:
Quinn A. Hastings, Jessica E. Davis, Sriram Kosuri, Eric M. Jones, Kimberly D. Insigne, W. Clifford Boldridge
Publikováno v:
Cell systems. 11(1)
In eukaryotes, transcription factors (TFs) orchestrate gene expression by binding to TF-binding sites (TFBSs) and localizing transcriptional co-regulators and RNA polymerase II to cis-regulatory elements. However, we lack a basic understanding of the
In eukaryotes, transcription factors orchestrate gene expression by binding to TF-Binding Sites (TFBSs) and localizing transcriptional co-regulators and RNA Polymerase II to cis-regulatory elements. The strength and regulation of transcription can be
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::843c7945967521d31e3c4fa27ac9aec3
https://doi.org/10.1101/625434
https://doi.org/10.1101/625434
Promoters are the key drivers of gene expression and are largely responsible for the regulation of cellular responses to time and environment. InE. coli,decades of studies have revealed most, if not all, of the sequence elements necessary to encode p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5310713985b2b9a4297fb0ee258df3f
https://europepmc.org/articles/PMC6389444/
https://europepmc.org/articles/PMC6389444/
Autor:
Eric M. Jones, David Yao, Sriram Kosuri, Kimberly D. Insigne, Rocky Cheung, Christina P. Burghard, Daniel B. Goodman
Publikováno v:
SSRN Electronic Journal.
Any individual’s genome contains ~4-5 million genetic variants that differ from reference, and understanding how these variants give rise to trait diversity and disease susceptibility is a central goal of human genetics (Auton et al., 2015). A vast
Autor:
Kimberly D. Insigne, Rocky Cheung, Christina P. Burghard, Daniel B. Goodman, Sriram Kosuri, David Yao, Eric M. Jones
Any individual’s genome contains ∼4-5 million genetic variants that differ from reference, and understanding how these variants give rise to trait diversity and disease susceptibility is a central goal of human genetics1. A vast majority (96-99%)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a124a840c659b2c4d22ffdb6c04b8d5a
Autor:
Eric M. Jones, Jeffrey C. Wang, Rocky Cheung, Christina P. Burghard, Daniel B. Goodman, David Yao, Xinshu Xiao, Yun-Hua Esther Hsiao, Sriram Kosuri, Kimberly D. Insigne
Publikováno v:
Molecular cell, vol 73, iss 1
Mutations that lead to splicing defects can have severe consequences on gene function and cause disease. Here, we explore how human genetic variation affects exon recognition by developing a multiplexed functional assay of splicing using Sort-seq (MF