Kinetochore component function in C. elegans oocytes revealed by 4D tracking of holocentric chromosomes

Autor: Laras Pitayu-Nugroho, Mélanie Aubry, Kimberley Laband, Hélène Geoffroy, Thadshagine Ganeswaran, Audi Primadhanty, Julie C. Canman, Julien Dumont

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)

Abstract During cell division, chromosome congression to the spindle center, their orientation along the spindle long axis and alignment at the metaphase plate depend on interactions between spindle microtubules and kinetochores, and are pre-requisit

Externí odkaz: https://doaj.org/article/76e5111ad4244ce283b1110644606a2c

Chromosome segregation occurs by microtubule pushing in oocytes

Autor: Kimberley Laband, Rémi Le Borgne, Frances Edwards, Marine Stefanutti, Julie C. Canman, Jean-Marc Verbavatz, Julien Dumont

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)

In oocytes of most species atypical spindles assembled in the absence of centrosomes drive chromosome segregation, however the forces driving this process are unclear. Here the authors found that spindle poles are largely dispensable and that inter-c

Externí odkaz: https://doaj.org/article/71f96c6c7ac34a67b76cccc61cc80a1b

Author response: Synergistic stabilization of microtubules by BUB-1, HCP-1, and CLS-2 controls microtubule pausing and meiotic spindle assembly

Autor: Frances Edwards, Kimberley Laband, Laura Bellutti, Nicolas Macaisne, Laras Pitayu-Nugroho, Alison Gervais, Thadshagine Ganeswaran, Hélène Geoffroy, Gilliane Maton, Julie C Canman, Benjamin Lacroix, Julien Dumont

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bdf4de915d1460a7b231a7ab5a19c123
https://doi.org/10.7554/elife.82579.sa2

Live imaging of C. elegans oocytes and early embryos

Autor: Kimberley, Laband, Benjamin, Lacroix, Frances, Edwards, Julie C, Canman, Julien, Dumont

Publikováno v: Methods in cell biology. 145

Caenorhabditis elegans is a self-fertilizing hermaphroditic worm. A single C. elegans worm therefore produces both male and female gametes that fuse to generate embryos. While sperm production stops at the end of the C. elegans larval development, oo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1f08fad0fed7ee4b5094c4a86157c0c9
https://pubmed.ncbi.nlm.nih.gov/29957205

Inhibition of ectopic microtubule assembly by the kinesin-13 KLP-7 prevents chromosome segregation and cytokinesis defects in oocytes

Autor: Frances Edwards, Emmanuelle Gigant, Julie P.I. Welburn, Benjamin Lacroix, Kimberley Laband, Julie C. Canman, Julien Dumont, Gilliane Maton, Marine Stefanutti, Agata Gluszek-Kustusz

Publikováno v: Gigant, E, Stefanutti, M, Laband, K, Gluszek-Kustusz, A, Edwards, F, Lacroix, B, Maton, G, Canman, J C, Welburn, J P I & Dumont, J 2017, ' Inhibition of ectopic microtubule assembly by the kinesin-13 KLP-7 prevents chromosome segregation and cytokinesis defects in oocytes ', Development, vol. 144, no. 9, pp. 1674-1686 . https://doi.org/10.1242/dev.147504

In most species, oocytes lack centrosomes. Accurate meiotic spindle assembly and chromosome segregation – essential to prevent miscarriage or developmental defects – thus occur through atypical mechanisms that are not well characterized. Using qu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::531f340e7e6b18ac543a2257e9640b6d
https://doi.org/10.1242/jcs.205914