Implementation of a high-throughput whole genome sequencing approach with the goal of maximizing efficiency and cost effectiveness to improve public health

Autor: Michelle C. Dickinson, Samantha E. Wirth, Deborah Baker, Anna M. Kidney, Kara K. Mitchell, Elizabeth J. Nazarian, Matthew Shudt, Lisa M. Thompson, Sai Laxmi Gubbala Venkata, Kimberlee A. Musser, Lisa Mingle

Publikováno v: Microbiology Spectrum, Vol 12, Iss 4 (2024)

ABSTRACTThis manuscript describes the development of a streamlined, cost-effective laboratory workflow to meet the demands of increased whole genome sequence (WGS) capacity while achieving mandated quality metrics. From 2020 to 2021, the Wadsworth Ce

Externí odkaz: https://doaj.org/article/e2126acd0ab248d4973b93305eb774fd

Novel Mycobacterium tuberculosis Complex Genotype Related to M. caprae

Autor: Joseph Shea, Carol Smith, Tanya A. Halse, Donna Kohlerschmidt, Amy K. Rourke, Kimberlee A. Musser, Vincent Escuyer, Pascal Lapierre

Publikováno v: Emerging Infectious Diseases, Vol 28, Iss 7, Pp 1431-1436 (2022)

We report the unusual genotypic characterization of a bacterium isolated from a clinical sample of a patient who grew up in Bangladesh and lives in the United States. Using whole-genome sequencing, we identified the bacterium as a member of the Mycob

Externí odkaz: https://doaj.org/article/bbc360a0d2ae4b43a583aeb620e02b00

The 2021 WHO catalogue of Mycobacterium tuberculosis complex mutations associated with drug resistance: a genotypic analysis

Autor: Timothy M Walker, DPhil, Paolo Miotto, PhD, Claudio U Köser, PhD, Philip W Fowler, PhD, Jeff Knaggs, BSc, Zamin Iqbal, DPhil, Martin Hunt, PhD, Leonid Chindelevitch, PhD, Maha R Farhat, MD, Daniela Maria Cirillo, PhD, Iñaki Comas, PhD, James Posey, PhD, Shaheed V Omar, PhD, Timothy EA Peto, ProfFRCP, Anita Suresh, MSc, Swapna Uplekar, PhD, Sacha Laurent, PhD, Rebecca E Colman, PhD, Carl-Michael Nathanson, PhD, Matteo Zignol, MD, Ann Sarah Walker, ProfPhD, Derrick W Crook, ProfFRCPath, Nazir Ismail, FRCPath [SA], Timothy C Rodwell, ProfMD, A Sarah Walker, Adrie J C Steyn, Ajit Lalvani, Alain Baulard, Alan Christoffels, Alberto Mendoza-Ticona, Alberto Trovato, Alena Skrahina, Alexander S Lachapelle, Alice Brankin, Amy Piatek, Ana Gibertoni Cruz, Anastasia Koch, Andrea Maurizio Cabibbe, Andrea Spitaleri, Angela P Brandao, Angkana Chaiprasert, Anita Suresh, Anna Barbova, Annelies Van Rie, Arash Ghodousi, Arnold Bainomugisa, Ayan Mandal, Aysha Roohi, Babak Javid, Baoli Zhu, Brice Letcher, Camilla Rodrigues, Camus Nimmo, Carl-Michael NATHANSON, Carla Duncan, Christopher Coulter, Christian Utpatel, Chunfa Liu, Clara Grazian, Clare Kong, Claudio U Köser, Daniel J Wilson, Daniela Maria Cirillo, Daniela Matias, Danielle Jorgensen, Danila Zimenkov, Darren Chetty, David AJ Moore, David A Clifton, Derrick W Crook, Dick van Soolingen, Dongxin Liu, Donna Kohlerschmidt, Draurio Barreira, Dumisani Ngcamu, Elias David Santos Lazaro, Ellis Kelly, Emanuele Borroni, Emma Roycroft, Emmanuel Andre, Erik C Böttger, Esther Robinson, Fabrizio Menardo, Flavia F Mendes, Frances B Jamieson, Francesc Coll, George Fu Gao, George W Kasule, Gian Maria Rossolini, Gillian Rodger, E Grace Smith, Graeme Meintjes, Guy Thwaites, Harald Hoffmann, Heidi Albert, Helen Cox, Ian F Laurenson, Iñaki Comas, Irena Arandjelovic, Ivan Barilar, Jaime Robledo, James Millard, James Johnston, Jamie Posey, Jason R Andrews, Jeff Knaggs, Jennifer Gardy, Jennifer Guthrie, Jill Taylor, Jim Werngren, John Metcalfe, Jorge Coronel, Joseph Shea, Joshua Carter, Juliana MW Pinhata, Julianne V Kus, Katharina Todt, Kathryn Holt, Kayzad S Nilgiriwala, Kelen T Ghisi, Kerri M Malone, Kiatichai Faksri, Kimberlee A Musser, Lavania Joseph, Leen Rigouts, Leonid Chindelevitch, Lisa Jarrett, Louis Grandjean, Lucilaine Ferrazoli, Mabel Rodrigues, Maha Farhat, Marco Schito, Margaret M Fitzgibbon, Marguerite Massinga Loembé, Maria Wijkander, Marie Ballif, Marie-Sylvianne Rabodoarivelo, Marina Mihalic, Mark WILCOX, Martin Hunt, Matteo ZIGNOL, Matthias Merker, Matthias Egger, Max O'Donnell, Maxine Caws, Mei-Hua Wu, Michael G Whitfield, Michael Inouye, Mikael Mansjö, Minh Ha Dang Thi, Moses Joloba, SM Mostofa Kamal, Nana Okozi, Nazir ISMAIL, Nerges Mistry, Nhung N Hoang, Niaina Rakotosamimanana, Nicholas I Paton, Paola M V Rancoita, Paolo Miotto, Pascal Lapierre, Patricia J Hall, Patrick Tang, Pauline Claxton, Penelope Wintringer, Peter M Keller, Phan Vuong Khac Thai, Philip W Fowler, Philip Supply, Prapaporn Srilohasin, Prapat Suriyaphol, Priti Rathod, Priti Kambli, Ramona Groenheit, Rebecca E Colman, Rick Twee-Hee Ong, Robin M Warren, Robert J Wilkinson, Roland Diel, Rosangela S Oliveira, Rukhsar Khot, Ruwen Jou, Sabira Tahseen, Sacha Laurent, Saheer Gharbia, Samaneh Kouchaki, Sanchi Shah, Sara Plesnik, Sarah G Earle, Sarah Dunstan, Sarah J Hoosdally, Satoshi Mitarai, Sebastien Gagneux, Shaheed V Omar, Shen-Yuan Yao, Simon Grandjean Lapierre, Simone Battaglia, Stefan Niemann, Sushil Pandey, Swapna Uplekar, Tanya A Halse, Ted Cohen, Teresa Cortes, Therdsak Prammananan, Thomas A Kohl, Nguyen T T Thuong, Tik Ying Teo, Timothy E A Peto, Timothy C Rodwell, Timothy William, Timothy M Walker, Thomas R Rogers, Utkarsha Surve, Vanessa Mathys, Victoria Furió, Victoria Cook, Srinivasan Vijay, Vincent Escuyer, Viola Dreyer, Vitali Sintchenko, Vonthanak Saphonn, Walter Solano, Wan-Hsuan Lin, Wayne van Gemert, Wencong He, Yang Yang, Yanlin Zhao, Youwen Qin, Yu-Xin Xiao, Zahra Hasan, Zamin Iqbal, Zully M Puyen

Publikováno v: The Lancet Microbe, Vol 3, Iss 4, Pp e265-e273 (2022)

Summary: Background: Molecular diagnostics are considered the most promising route to achievement of rapid, universal drug susceptibility testing for Mycobacterium tuberculosis complex (MTBC). We aimed to generate a WHO-endorsed catalogue of mutation

Externí odkaz: https://doaj.org/article/5404bb0e9de24bc8bc42f357dff6c954

Nanopore MinION Sequencing Reveals Possible Transfer of blaKPC–2 Plasmid Across Bacterial Species in Two Healthcare Facilities

Autor: Catharine Prussing, Emily A. Snavely, Navjot Singh, Pascal Lapierre, Erica Lasek-Nesselquist, Kara Mitchell, Wolfgang Haas, Rita Owsiak, Elizabeth Nazarian, Kimberlee A. Musser

Publikováno v: Frontiers in Microbiology, Vol 11 (2020)

Carbapenemase-producing Enterobacteriaceae are a major threat to global public health. Klebsiella pneumoniae carbapenemase (KPC) is the most commonly identified carbapenemase in the United States and is frequently found on mobile genetic elements inc

Externí odkaz: https://doaj.org/article/683797006d904e419e5581f32512bdc4

Genomic Analysis of Vancomycin-Resistant Staphylococcus aureus Isolates from the 3rd Case Identified in the United States Reveals Chromosomal Integration of the vanA Locus

Autor: Wolfgang Haas, Navjot Singh, William Lainhart, Lisa Mingle, Elizabeth Nazarian, Kara Mitchell, Geetha Nattanmai, Donna Kohlerschmidt, Michelle C. Dickinson, Marilyn Kacica, Nellie Dumas, Kimberlee A. Musser

Publikováno v: Microbiology Spectrum. 11

High-level vancomycin-resistant Staphylococcus aureus (VRSA) began to emerge in the United States in 2002 and has since then been reported worldwide. Our study reports the closed genome sequences of multiple VRSA isolates obtained in 2004 from a sing

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d96cf8470c95ba1888975d7c9d13c2ba
https://doi.org/10.1128/spectrum.04317-22