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Akademický článek

Adaptive evolution of a minimal organism with a synthetic genome

Autor: Troy E. Sandberg, Kim S. Wise, Christopher Dalldorf, Richard Szubin, Adam M. Feist, John I. Glass, Bernhard O. Palsson

Publikováno v: iScience, Vol 26, Iss 9, Pp 107500- (2023)

Summary: The bacterial strain JCVI-syn3.0 stands as the first example of a living organism with a minimized synthetic genome, derived from the Mycoplasma mycoides genome and chemically synthesized in vitro. Here, we report the experimental evolution

Externí odkaz: https://doaj.org/article/1feada2cbb2c4658a2ca0337974eaee2

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Akademický článek

Metabolite Damage and Damage Control in a Minimal Genome

Publikováno v: mBio, Vol 13, Iss 4 (2022)

ABSTRACT Analysis of the genes retained in the minimized Mycoplasma JCVI-Syn3A genome established that systems that repair or preempt metabolite damage are essential to life. Several genes known to have such functions were identified and experimental

Externí odkaz: https://doaj.org/article/71126318982049979973bef57993dd2a

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Akademický článek

Essential metabolism for a minimal cell

Autor: Marian Breuer, Tyler M Earnest, Chuck Merryman, Kim S Wise, Lijie Sun, Michaela R Lynott, Clyde A Hutchison, Hamilton O Smith, John D Lapek, David J Gonzalez, Valérie de Crécy-Lagard, Drago Haas, Andrew D Hanson, Piyush Labhsetwar, John I Glass, Zaida Luthey-Schulten

Publikováno v: eLife, Vol 8 (2019)

JCVI-syn3A, a robust minimal cell with a 543 kbp genome and 493 genes, provides a versatile platform to study the basics of life. Using the vast amount of experimental information available on its precursor, Mycoplasma mycoides capri, we assembled a

Externí odkaz: https://doaj.org/article/8a4a0da0c3184384beb1ab8154468210

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Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-tree gene expression platform

Autor: Andrei Sakai, Christopher R Deich, Frank H T Nelissen, Aafke J Jonker, Daniela M de C Bittencourt, Christopher P Kempes, Kim S Wise, Hans A Heus, Wilhelm T S Huck, Katarzyna P Adamala, John I Glass

Publikováno v: Synthetic Biology, 7, 1, pp. 1-14
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
Synthetic Biology, 7, 1-14

Cell-free expression (CFE) systems are one of the main platforms for building synthetic cells. A major drawback is the orthogonality of cell-free systems across species. To generate a CFE system compatible with recently established minimal cell const

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16305d9546f890b7a0497a9116429293

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Metabolite Damage and Damage-Control in a Minimal Genome

Analysis of the genes retained in the minimized Mycoplasma JCVI-Syn3A genome established that systems that repair or preempt metabolite damage are essential to life. Several genes with known metabolite damage repair or preemption functions were ident

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1609a03e8cc1fb162cbefb85faee77e3
https://doi.org/10.1101/2021.12.01.470718

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Evolution of a minimal cell

Autor: Michael Lynch, Jay T. Lennon, John I. Glass, Daniela Bittencourt, Kim S. Wise, Roy Z. Moger-Reischer, Lijie Sun

Possessing only essential genes, a minimal cell can reveal mechanisms and processes that are critical for the persistence and stability of life. Here, we report on how a synthetically constructed minimal cell contends with the forces of evolution com

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f5156a16385a08dd68c30cd11d060a2e
https://doi.org/10.1101/2021.06.30.450565

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