Structure and dimerization of translation initiation factor aIF5B in solution

Autor: Louise Carøe Vohlander Rasmussen, Janni M Jensen, Jan Skov Pedersen, Hans Uffe Sperling-Petersen, Cristiano L. P. Oliveira, Olwyn Byron, Kim Kusk Mortensen

Publikováno v: Rasmussen, L C V, Oliveira, C L P, Byron, O, Jensen, J M, Pedersen, J S, Sperling-Petersen, H U & Mortensen, K K 2011, ' Structure and dimerization of translation initiation factor aIF5B in solution ', Biochemical and Biophysical Research Communications, vol. 416, pp. 140-145 . https://doi.org/10.1016/j.bbrc.2011.11.012

Translation initiation factor 5B (IF5B) is required for initiation of protein synthesis. The solution structure of archaeal IF5B (aIF5B) was analysed by small-angle X-ray scattering (SAXS) and dynamic light scattering (DLS) and was indicated to be in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ac7ad6036a92477041983f13cda6d5e
https://doi.org/10.1016/j.bbrc.2011.11.012

Enantioselective Proteins: Selection, Binding Studies and Molecular Modeling of Antibodies with Affinity towards Hydrophobic BINOL Derivatives

Autor: Jesper Givskov Sørensen, Kim Kusk Mortensen, Troels Skrydstrup, Janni Egebjerg, Birgit Schiøtt, Jan Pedersen, Brian Schou Rasmussen

Publikováno v: Rasmussen, B S, Pedersen, J M, Sørensen, J, Egebjerg, J, Schiøtt, B, Mortensen, K K & Skrydstrup, T 2007, ' Enantioselective Proteins: Selection, Binding Studies and Molecular Modeling of Antibodies with Affinity towards Hydrophobic BINOL Derivatives ', ChemBioChem, vol. 8, no. 16, pp. 1974-1980 . https://doi.org/10.1002/cbic.200700295

In this paper, the initial steps towards the design of novel artificial metalloenzymes that exploit proteins as a second coordination sphere for traditional metal-ligand catalysis are described. Phage display was employed to select and study antibody

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ea612973a02c23468094b55a5d40187
https://doi.org/10.1002/cbic.200700295

The N-terminal domain (IF2N) of bacterial translation initiation factor IF2 is connected to the conserved C-terminal domains by a flexible linker

Autor: Brian Søgaard Laursen, Anne Cecillie Kjærgaard, Hans Uffe Sperling-Petersen, Kim Kusk Mortensen, David W. Hoffman

Publikováno v: Protein Science. 13:230-239

Bacterial translation initiation factor IF2 is a multidomain protein that is an essential component of a system for ensuring that protein synthesis begins at the correct codon within a messenger RNA. Full-length IF2 from Escherichia coli and seven fr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b921cbb270b8007c425316d357ba300
https://doi.org/10.1110/ps.03337604

A Conserved Structural Motif at the N Terminus of Bacterial Translation Initiation Factor IF2

Autor: Brian Søgaard Laursen, Hans Uffe Sperling-Petersen, David W. Hoffman, Kim Kusk Mortensen

Publikováno v: Journal of Biological Chemistry. 278:16320-16328

The 18-kDa Domain I from the N-terminal region of translation initiation factor IF2 from Escherichia coli was expressed, purified, and structurally characterized using multidimensional NMR methods. Residues 2-50 were found to form a compact subdomain

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ebb5a009c4ad1802203a0825878b7da
https://doi.org/10.1074/jbc.m212960200

Characterization of Mutations in the GTP-binding Domain of IF2 Resulting in Cold-sensitive Growth of Escherichia coli

Autor: Brian Søgaard Laursen, Igor Siwanowicz, Guilhem Larigauderie, John M. Kenney, Koreaki Ito, Yoshikazu Nakamura, Kim Kusk Mortensen, Hans Uffe Sperling-Petersen, Jakob Hedegaard

Publikováno v: Laursen, B S, Siwanowicz, I, Larigauderie, G, Hedegaard, J, Ito, K, Nakamura, Y, Kenney, J M, Mortensen, K K & Sperling-Petersen, H U 2003, ' Characterization of mutations in the GTP-binding domain of IF2 resulting in cold-sensitive growth of Escherichia coli ', J. Mol. Biol., vol. 326, pp. 543-551 .
Laursen, B S, Siwanowicz, I, Larigauderie, G, Hedegaard, J, Ito, K, Nakamura, Y, Kenney, J M, Mortensen, K K & Sperling-Petersen, H U 2003, ' Characterization of Mutations in the GTP-binding Domain of IF2 Resulting in Cold-sensitive Growth of Escherichia coli ', Journal of Molecular Biology, vol. 326, no. 2, pp. 543-551 . https://doi.org/10.1016/S0022-2836(02)01367-0
Laursen, B S, Siwanowicz, I, Larigauderie, G, Hedegaard, J, Ito, K, Nakamura, Y, Kenney, J M, Mortensen, K K & Sperling-Petersen, H U 2003, ' Characterization of mutations in the GTP-binding domain of IF2 resulting in cold-sensitive growth of Escherichia coli ', Journal of Molecular Biology, vol. 326 .

The infB gene encodes translation initiation factor IF2. We have determined the entire sequence of infB from two cold-sensitive Escherichia coli strains IQ489 and IQ490. These two strains have been isolated as suppressor strains for the temperature-s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d67307e87e348b5e3fb7999bcde6c40
https://doi.org/10.1016/s0022-2836(02)01367-0