Activity-Based DNA-Encoded Library Screening for Selective Inhibitors of Eukaryotic Translation

Autor: Huda Barhoosh, Anjali Dixit, Wesley G. Cochrane, Valerie Cavett, Robin N. Prince, Brooke O. Blair, Fred R. Ward, Kim F. McClure, Phillip A. Patten, Margot G. Paulick, Brian M. Paegel

Publikováno v: ACS Central Science, Vol 10, Iss 10, Pp 1960-1968 (2024)

Externí odkaz: https://doaj.org/article/54226ab724d245ef9672211b878dc5e8

Selective stalling of human translation through small-molecule engagement of the ribosome nascent chain.

Autor: Nathanael G Lintner, Kim F McClure, Donna Petersen, Allyn T Londregan, David W Piotrowski, Liuqing Wei, Jun Xiao, Michael Bolt, Paula M Loria, Bruce Maguire, Kieran F Geoghegan, Austin Huang, Tim Rolph, Spiros Liras, Jennifer A Doudna, Robert G Dullea, Jamie H D Cate

Publikováno v: PLoS Biology, Vol 15, Iss 3, p e2001882 (2017)

Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a key role in regulating the levels of plasma low-density lipoprotein cholesterol (LDL-C). Here, we demonstrate that the compound PF-06446846 inhibits translation of PCSK9 by inducing the ri

Externí odkaz: https://doaj.org/article/c8c20b848f9a4b7e848edcb19074a5c8

Structural basis for selective stalling of human ribosome nascent chain complexes by a drug-like molecule

Autor: Spiros Liras, Elizabeth Montabana, Wenfei Li, Jamie H. D. Cate, Kim F. McClure, Stacey Tsai-Lan Chang, Robert Dullea, Fred R. Ward

Publikováno v: Nature structural & molecular biology

Small molecules that target the ribosome generally have a global impact on protein synthesis. However, the drug-like molecule PF-06446846 (PF846) binds the human ribosome and selectively blocks the translation of a small subset of proteins by an unkn

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::732655f3f54240d1916224047ea54bc4
https://doi.org/10.1038/s41594-019-0236-8

Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing

Autor: Ariana Hirsh, Robert Dullea, Meihua Tu, Spiros Liras, Joan Compte Barrón, Kris A. Borzilleri, Nannan Ma, Rima Mendonsa, Justin Bellenger, Romain Rouet, Lorena de Oñate, Xidong Feng, Jennifer A. Doudna, Nathanael G. Lintner, David M. Rubitski, Benjamin A. Thuma, Marc D. Roy, Alison H. Varghese, Kim F. McClure, Ross C. Wilson, Thomas J. McLellan, Hanna M. Wisniewska, James E. Finley, Boris A. Chrunyk, Vincent Mascitti, Kaihong Zhou, Kevin D. Hesp

Publikováno v: Journal of the American Chemical Society, vol 140, iss 21
Rouet, Romain; Thuma, Benjamin A; Roy, Marc D; Lintner, Nathanael G; Rubitski, David M; Finley, James E; et al.(2018). Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing.. Journal of the American Chemical Society, 140(21), 6596-6603. doi: 10.1021/jacs.8b01551. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/61k3t664

CRISPR-Cas RNA-guided endonucleases hold great promise for disrupting or correcting genomic sequences through site-specific DNA cleavage and repair. However, the lack of methods for cell- and tissue-selective delivery currently limits both research a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9af967c5dc058dcd0e0a6cfe05eb8b81
https://doi.org/10.1021/jacs.8b01551

Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease

Autor: Jing Zhou, Khetemenee Lam, Romer A. Gonzalez-Villalobos, John J. Scarcelli, Eric Sousa, Yan Weng, Ron Kriz, Tetsuya Ishino, Darren Ferguson, Yan Zhang, Hongli L. Zhu, Boriana Tzvetkova, Valerie Clerin, Mellisa Ly, Alan C. Opsahl, Xiaotian Zhong, Qing Yao, Kim F. McClure, Kaffa Cote, Aaron M. D’Antona, Justin Cohen, Laura Lin, Annette Sievers, Weili Duan, James R. Apgar, Jason C. Rouse, Srinath Jagarlapudi, Joe Palandra, Keith A. Johnson, Suma Yaddanapudi, Richard Zollner

Publikováno v: J Biol Chem

The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD). Klotho functions as a c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2aa95052a8ec3a97f4bd0138f1cc2d5
https://pubmed.ncbi.nlm.nih.gov/32005658