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Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids

Autor: Bojmar, Linda, Kim, Han Sang, Tobias, Gabriel C., Pelissier Vatter, Fanny A., Lucotti, Serena, Gyan, Kofi Ennu, Kenific, Candia M., Wan, Zurong, Kim, Kyung-A, Kim, DooA, Hernandez, Jonathan, Pascual, Virginia, Heaton, Todd E., La Quaglia, Michael P., Kelsen, David, Trippett, Tanya M., Jones, David R., Jarnagin, William R., Matei, Irina R., Zhang, Haiying, Hoshino, Ayuko, Lyden, David

Publikováno v: In STAR Protocols 19 March 2021 2(1)

Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids.

Autor: Bojmar L; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Biomedical and Clinical Sciences, Linköping University, Linköping 58183, Sweden., Kim HS; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Yonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea., Tobias GC; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil., Pelissier Vatter FA; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA., Lucotti S; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA., Gyan KE; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Tri-Institutional PhD Program in Computational Biology and Medicine, New York, NY, USA., Kenific CM; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA., Wan Z; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; Department of Pediatrics, Weill Cornell Medicine, New York, NY, USA., Kim KA; Yonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea., Kim D; Yonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea., Hernandez J; Hepatopancreatobiliary Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA.; Surgical Oncology Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Pascual V; Department of Pediatrics, Weill Cornell Medicine, New York, NY, USA.; Drukier Institute for Children's Health, Weill Cornell Medicine, New York, NY, USA., Heaton TE; Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.; Pediatric Surgical Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA., La Quaglia MP; Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.; Pediatric Surgical Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Kelsen D; Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Trippett TM; Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Jones DR; Thoracic Surgery Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Jarnagin WR; Hepatopancreatobiliary Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Matei IR; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA., Zhang H; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA., Hoshino A; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.; School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan., Lyden D; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.

Publikováno v: STAR protocols [STAR Protoc] 2020 Dec 22; Vol. 2 (1), pp. 100225. Date of Electronic Publication: 2020 Dec 22 (Print Publication: 2021).