Zobrazeno 1 - 10
of 51
pro vyhledávání: '"Kiersten M. Ruff"'
Publikováno v:
Nucleus, Vol 15, Iss 1 (2024)
ABSTRACTIn higher eukaryotes, the nucleolus harbors at least three sub-phases that facilitate multiple functionalities including ribosome biogenesis. The three prominent coexisting sub-phases are the fibrillar center (FC), the dense fibrillar compone
Externí odkaz:
https://doaj.org/article/d1a5e3c5f5074299832f797e96ef7638
Autor:
Andrew Z. Lin, Kiersten M. Ruff, Furqan Dar, Ameya Jalihal, Matthew R. King, Jared M. Lalmansingh, Ammon E. Posey, Nadia A. Erkamp, Ian Seim, Amy S. Gladfelter, Rohit V. Pappu
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-17 (2023)
Abstract Cellular matter can be organized into compositionally distinct biomolecular condensates. For example, in Ashbya gossypii, the RNA-binding protein Whi3 forms distinct condensates with different RNA molecules. Using criteria derived from a phy
Externí odkaz:
https://doaj.org/article/637a2e86d36b45be88e92a46689153be
Autor:
Hilda Mirbaha, Dailu Chen, Olga A Morazova, Kiersten M Ruff, Apurwa M Sharma, Xiaohua Liu, Mohammad Goodarzi, Rohit V Pappu, David W Colby, Hamid Mirzaei, Lukasz A Joachimiak, Marc I Diamond
Publikováno v:
eLife, Vol 7 (2018)
Tauopathies feature progressive accumulation of tau amyloids. Pathology may begin when these amplify from a protein template, or seed, whose structure is unknown. We have purified and characterized distinct forms of tau monomer—inert (Mi) and seed-
Externí odkaz:
https://doaj.org/article/4a281ab9d29a4287a093526308f4f1c5
Autor:
Matthew R. King, Andrew Z. Lin, Kiersten M. Ruff, Mina Farag, Wei Ouyang, Michael D. Vahey, Emma Lundberg, Rohit V. Pappu
The nucleolus is a multilayered structure. Each layer is thought to be a compositionally distinct phase, although how these phases form and interface with one another remains unclear. Using computational, proteomics,in vitro, andin vivostudies, we un
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::289ab7b7c2ad6c0b018c9164a512d72e
https://doi.org/10.1101/2022.11.05.515292
https://doi.org/10.1101/2022.11.05.515292
Autor:
Min Kyung Shinn, Megan C. Cohan, Jessie L. Bullock, Kiersten M. Ruff, Petra A. Levin, Rohit V. Pappu
Publikováno v:
Proceedings of the National Academy of Sciences. 119
Intrinsically disordered regions (IDRs) can function as autoregulators of folded enzymes to which they are tethered. One example is the bacterial cell division protein FtsZ. This includes a folded core and a C-terminal tail (CTT) that encompasses a p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2270d7fb79aafd90f28050d8c6a87306
https://doi.org/10.1073/pnas.2211178119
https://doi.org/10.1073/pnas.2211178119
Autor:
Min Kyung Shinn, Megan C. Cohan, Jessie L. Bullock, Kiersten M. Ruff, Petra A. Levin, Rohit V. Pappu
Publikováno v:
Biophysical Journal. 122:60a-61a
Intrinsically disordered regions (IDRs) can function as autoregulators of folded enzymes to which they are tethered. One example is the bacterial cell division protein, FtsZ. This includes a folded core and a C-terminal tail (CTT) that encompasses a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::748752fbc187d6bc7922079ec24f1fa0
https://doi.org/10.1016/j.bpj.2022.11.535
https://doi.org/10.1016/j.bpj.2022.11.535
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 119(19)
The most commonly occurring intrinsically disordered proteins (IDPs) are polyampholytes, which are defined by the duality of low net charge per residue and high fractions of charged residues. Recent experiments have uncovered nuances regarding sequen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::286151f004edf4bdf5f70d465c40d046
https://pubmed.ncbi.nlm.nih.gov/35512095
https://pubmed.ncbi.nlm.nih.gov/35512095
Autor:
Rohit V. Pappu, Andrew Z. Lin, Kiersten M. Ruff, Ameya P. Jalihal, Furqan Dar, Ammon E. Posey, Matthew R. King, Jared M. Lalmansingh, Amy S. Gladfelter
Publikováno v:
Biophysical Journal. 122:443a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9d09410500f44bff7a49b7fba7abf655
https://doi.org/10.1016/j.bpj.2022.11.2390
https://doi.org/10.1016/j.bpj.2022.11.2390
Autor:
Kiersten M. Ruff, Yoon Hee Choi, Dezerae Cox, Angelique R. Ormsby, Yoochan Myung, David B. Ascher, Sheena E. Radford, Rohit V. Pappu, Danny M. Hatters
Publikováno v:
Molecular Cell. 82:3193-3208.e8
Aberrant phase separation of globular proteins is associated with many diseases. Here, we use a model protein system to understand how the unfolded states of globular proteins drive phase separation and the formation of unfolded protein deposits (UPO
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c7e0fc899485f2eb7f1c898f3f18d52
https://doi.org/10.1016/j.molcel.2022.06.024
https://doi.org/10.1016/j.molcel.2022.06.024
Autor:
Kiersten M. Ruff, Danny M. Hatters, Dezerae Cox, Yoochan Myung, David B. Ascher, Rohit V. Pappu, Yoon Hee Choi, Sheena E. Radford, Angelique R. Ormsby
Protein homeostasis involves regulation of the concentrations of unfolded states of globular proteins. Dysregulation can cause phase separation leading to protein-rich deposits. Here, we uncover the sequence-grammar that influences the triad of foldi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9036851a42c4da648939db5c5e780cbc
https://doi.org/10.1101/2021.08.20.457073
https://doi.org/10.1101/2021.08.20.457073