Zobrazeno 1 - 10
of 59
pro vyhledávání: '"Kichiko Koike"'
Autor:
Kichiko Koike
Publikováno v:
Journal of Clinical Biochemistry and Nutrition. 30:67-77
Highly sensitive and rapid assay procedures for human mitochondrial the pyruvate dehydrogenase (PDH) complex, the 2-oxoglutarate dehydrogenase (OGDH) complex and their 5 component enzymes were established for use with crude tissue extracts, and their
Publikováno v:
European Journal of Biochemistry. 267:3005-3016
Dihydrolipoamide succinyltransferase (E2o) is the structural and catalytic core of the 2-oxoglutarate dehydrogenase (OGDH) complex. The cDNA encoding porcine E2o (PE2o) has been cloned. The PE2o cDNA spans 2547 bases encoding a presequence (68 amino-
Autor:
Kichiko Koike
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1385:373-384
Human 2-oxoglutarate dehydrogenase (OGDH) is an E1-component of the OGDH multi-enzyme complex and catalyzes both the ThDP-dependent decarboxylation of 2-oxoglutarate and the subsequent reductive succinylation of the lipoyl moiety which is covalently
Autor:
Kichiko Koike
Publikováno v:
Gene. 159:261-266
Genomic clones covering the entire sequence of the gene encoding human 2-oxoglutarate dehydrogenase (OGDH) were isolated by screening leukocyte and placenta genomic libraries with radio- and digoxigenin (DIG)-labeled human OGDH cDNAs. The human OGDH
Autor:
Kichiko Koike, Kazuhiro Hayashida, Minoru Nakamura, Hiromi Ishibashi, Yoshiyuki Niho, Shinji Shimoda, Mieko Matsui
Publikováno v:
Autoimmunity. 21:253-262
B lymphocytes committed to the production of IgG antibodies (Abs) to mitochondrial antigen such as pyruvate dehydrogenase complex(PDC) were quantitated in the peripheral blood of patients with primary biliary cirrhosis(PBC) using Epstein-Barr virus a
Autor:
Minoru Nakamura, Hiromi Ishibashi, Kichiko Koike, Hideyuki Ikematsu, Kazuhiro Hayashida, Yoshiyuki Niho, Nobuyoshi Fukushima, Mieko Matsui
Publikováno v:
International Immunology. 7:1047-1055
We established one Epstein-Barr virus-transformed B cell hybrid clone producing human mAb of the IgG class to the 2-oxoglutarate dehydrogenase complex (OGDC) for the first time from the peripheral B lymphocytes of a patient with primary biliary cirrh
Publikováno v:
Archives of Dermatological Research. 284:283-289
Unrepaired cyclobutane-type pyrimidine dimers of DNA extracted from human skin tumours were examined by an immunoblotting method using polyclonal antibodies raised against UV-irradiated calf thymus DNA. A total of 40 DNA samples extracted from seven
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1118:223-230
Porcine heart muscle pyruvate dehydrogenase (PDH, EC 1.2.4.1) with subunit composition alpha 2 beta 2 catalyzes the initial decarboxylation step of an oxidative decarboxylation sequence of pyruvate. Highly purified PDH, was further activated several-
Publikováno v:
Journal of Clinical Biochemistry and Nutrition. 13:211-221
A Japanese boy with fatal pyruvate dehydrogenase deficiency died at 93h of age despite intensive treatment for a progressive metabolic acidosis and hyperammonemia. Enzymatic studies of the patient's liver and kidney homogenates revealed a decreased P
Publikováno v:
Journal of Clinical Biochemistry and Nutrition. 9:151-161
A novel method for the microquantitative simultaneous determination of lactate and pyruvate in blood plasma and urine is described. The principle of the assay is to determine lactate after its enzymatic conversion to pyruvate followed by the derivati